A synthetic peptide from AA 450-510 of human VACHT conjugated to blue carrier protein was used as the antigen. The peptide is homologous in rat and mouse.
IHC, WB. A working concentration of 1-10 μg,ml is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications.
限制
仅限研究用
状态
Lyophilized
溶解方式
Reconstitute in 50 µL of sterile water. Centrifuge to remove any insoluble material.
注意事项
Avoid freeze and thaw cycles.
储存条件
4 °C/-20 °C
储存方法
Maintain the lyophilised/reconstituted antibodies frozen at -20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles.
有效期
12 months
抗原
SLC18A3
(Solute Carrier Family 18 (Vesicular Acetylcholine), Member 3 (SLC18A3))
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背景
FUNCTION: Involved in acetylcholine transport into synaptic vesicles. SUBCELLULAR LOCATION: Membrane, Multi-pass membrane protein. TISSUE SPECIFICITY: High expression in all major cholinergic cell groups, including peripheral postganglionic parasympathetic cells, preganglionic sympathetic and parasympathetic cells, ventral spinal cord and brainstem motoneurons, cell groups in the basal forebrain, the habenula and the corpus striatum. Weakly expressed in the cortex and hippocampus. VACHT is also present in the olfactory bulb, nucleus of the lateral olfactory tract, olfactory tubercle. SIMILARITY: Belongs to the major facilitator superfamily. Vesicular transporter family.,SLC18 Family,Vesicular acetylcholine transporter, solute carrier family 18 member 3, SLC18A3, MGC12716