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TJP1 抗体 (AA 463-1109)

TJP1 适用: 人, 小鼠, 大鼠, 犬, 豚鼠 WB, IHC, ELISA, IP, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN305736
发货至: 中国
  • 抗原 See all TJP1 抗体
    TJP1 (Tight Junction Protein 1 (TJP1))
    抗原表位
    • 16
    • 6
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 463-1109
    适用
    • 52
    • 31
    • 29
    • 20
    • 18
    • 8
    • 5
    • 5
    • 4
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    人, 小鼠, 大鼠, 犬, 豚鼠
    宿主
    • 48
    • 3
    • 2
    • 1
    • 1
    克隆类型
    • 50
    • 5
    多克隆
    标记
    • 37
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This TJP1 antibody is un-conjugated
    应用范围
    • 50
    • 20
    • 17
    • 14
    • 14
    • 13
    • 11
    • 11
    • 10
    • 7
    • 7
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))
    品牌
    IHC-plus™
    特异性
    Recognizes human ZO-1alpha+ and ZO- alpha- isoforms. Reactivity with dlg has not been characterized. No reactivity with PSD-95 or p55 has been observed on Western blots, but potential reactivity with these proteins has not been fully characterized. Predicted species cross-reactivity: mouse, rat, guinea pig, canine and chicken (moderate reactivity). Please note that cross-reactivity with predicted species is only predicted and is not guaranteed.
    纯化方法
    Immunoaffinity purified
    免疫原
    A 69kD fusion protein corresponding to aa463-1109 of human ZO-1 cDNA. This sequence lies N-terminal to the 80 amino acid region (the alpha-motif) present in the a+-isoform but absent in the a- isoform due to alternative splicing.

    Type of Immunogen: Fusion protein
    亚型
    IgG
    Top Product
    Discover our top product TJP1 Primary Antibody
  • 应用备注
    Approved: ELISA, IHC, IHC-Fr, IHC-P (5 μg/mL), IP, WB (0.5 - 2 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: 0.5-2 μg/mL. Immunoprecipitation: 2-5 μg/reaction.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    PBS,  pH 7.4, 0.09 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeat freeze-thaw cycles.
    储存条件
    4 °C,-20 °C
    储存方法
    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • 抗原
    TJP1 (Tight Junction Protein 1 (TJP1))
    别名
    TJP1 / ZO-1 (TJP1 产品)
    别名
    ZO-1 antibody, ZO1 antibody, zo1 antibody, tight junction protein 1 antibody, tight junction protein ZO-1 antibody, TJP1 antibody, Tjp1 antibody, LOC396567 antibody
    背景
    Name/Gene ID: TJP1
    Family: Occludin

    Synonyms: TJP1, ZO-1, Zona occludens protein 1, Zona occludens 1, Zonula occludens protein 1, Tight junction protein 1, ZO1, Tight junction protein ZO-1, Zonula occludens 1 protein
    基因ID
    7082
    UniProt
    Q07157
    途径
    Carbohydrate Homeostasis, Cell-Cell Junction Organization
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