AIF 抗体 (C-Term)
(3 references)-
- 抗原 See all AIF (AIFM1) 抗体
- AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))
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抗原表位
- AA 596-613, C-Term
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This AIF antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 原理
- Rabbit IgG polyclonal antibody for Apoptosis-inducing factor 1, mitochondrial(AIFM1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
- 序列
- EQHEDLNEVA KLFNIHED
- 交叉反应 （详细）
- No cross reactivity with other proteins.
- 产品特性
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Rabbit IgG polyclonal antibody for Apoptosis-inducing factor 1, mitochondrial(AIFM1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: apoptosis-inducing factor, mitochondrion-associated, 1
Protein Name: Apoptosis-inducing factor 1, mitochondrial - 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human AIF(596-613aa EQHEDLNEVAKLFNIHED), identical to the related rat and mouse sequences.
- 亚型
- IgG
- Top Product
- Discover our top product AIFM1 Primary Antibody
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anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (AA 131-230) antibody
AIFM1 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, IHC (p), ICC, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) antibodyAIFM1 适用: 人, 小鼠, 大鼠, 猴 WB, ELISA, IHC, ICC, FACS 宿主: 小鼠 Monoclonal 4-00E-07 unconjugated
anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (C-Term) antibodyVerified AIFM1 适用: 人, 小鼠, 大鼠, Pig WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated
anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (AA 334-613) antibodyAIFM1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (AA 103-322) antibodyAIFM1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (AA 183-195) antibodyVerified AIFM1 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 山羊 Polyclonal unconjugated
anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (AA 103-612) antibodyAIFM1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (AA 1-613) antibodyAIFM1 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (AA 1-613) antibodyAIFM1 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1) (C-Term) antibodyAIFM1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Mouse, Rat
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- 储存液
- Thimerosal (Merthiolate), Sodium azide
- 注意事项
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - 有效期
- 12 months
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: "Mechanism of apoptosis induction in human hepatocellular carcinoma cells following treatment with a gecko peptides mixture." in: Biomedical reports, Vol. 5, Issue 1, pp. 73-78, (2016) (PubMed).
: "Wnt5a Increases Properties of Lung Cancer Stem Cells and Resistance to Cisplatin through Activation of Wnt5a/PKC Signaling Pathway." in: Stem cells international, Vol. 2016, pp. 1690896, (2016) (PubMed).
: "Protective effects of nigranoic acid on cerebral ischemia-reperfusion injury and its mechanism involving apoptotic signaling pathway." in: Cell biochemistry and biophysics, Vol. 71, Issue 1, pp. 345-51, (2015) (PubMed).
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: "Mechanism of apoptosis induction in human hepatocellular carcinoma cells following treatment with a gecko peptides mixture." in: Biomedical reports, Vol. 5, Issue 1, pp. 73-78, (2016) (PubMed).
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- 抗原
- AIF (AIFM1) (Apoptosis-Inducing Factor, Mitochondrion-Associated, 1 (AIFM1))
- 别名
- AIFM1 (AIFM1 产品)
- 别名
- AIF antibody, CMTX4 antibody, COWCK antibody, COXPD6 antibody, PDCD8 antibody, CG7263 antibody, DmAIF antibody, Dmel\\CG7263 antibody, GB16024 antibody, DDBDRAFT_0187853 antibody, DDBDRAFT_0191137 antibody, DDB_0187853 antibody, DDB_0191137 antibody, aif antibody, pdcd8 antibody, AIFM1 antibody, PCD8 antibody, AIFsh2 antibody, Hq antibody, Pdcd8 antibody, mAIF antibody, Aif antibody, zgc:91994 antibody, apoptosis inducing factor mitochondria associated 1 antibody, allograft inflammatory factor 1 antibody, Apoptosis inducing factor antibody, apoptosis-inducing factor 1, mitochondrial antibody, apoptosis inducing factor antibody, apoptosis inducing factor, mitochondria associated 1 antibody, apoptosis-inducing factor, mitochondrion-associated, 1 antibody, apoptosis-inducing factor, mitochondrion-associated 1 antibody, AIFM1 antibody, AIF1 antibody, AIF antibody, LOC412212 antibody, aif antibody, aifm1 antibody, Aifm1 antibody
- 背景
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Apoptosis-inducing factor 1, mitochondrial, also known as AIF or PDCD8 is a protein that in humans is encoded by the AIFM1 gene. AIFM1 gene is mapped to Xq26.1 based on an alignment of the AIFM1 sequence with the genomic sequence. This gene encodes a flavoprotein essential for nuclear disassembly in apoptotic cells, and it is found in the mitochondrial intermembrane space in healthy cells. Induction of apoptosis results in the translocation of this protein to the nucleus where it affects chromosome condensation and fragmentation. In addition, this gene product induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. Mutations in this gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. A related pseudogene has been identified on chromosome 10.
Synonyms: AIFM1 antibody|Apoptosis inducing factor 1, mitochondrial antibody|Apoptosis inducing factor antibody|Apoptosis inducing factor, mitochondrion associated, 1 antibody|Apoptosis-inducing factor 1 antibody|CMTX4 antibody|COXPD6 antibody|Harlequin antibody|Hq antibody|mAIF antibody|MGC111425 antibody|MGC5706 antibody|mitochondrial antibody|PDCD 8 antibody|PDCD8 antibody|Programmed cell death 8(apoptosis inducing factor) antibody|Programmed cell death 8 antibody|Programmed cell death 8 isoform 1 antibody|Programmed cell death 8 isoform 2 antibody|Programmed cell death 8 isoform 3 antibody|Programmed cell death 8 isoform 5 antibody|Programmed cell death protein 8 antibody|Programmed cell death protein 8 mitochondrial antibody|Programmed cell death protein 8 mitochondrial precursor antibody|Striatal apoptosis inducing factor antibody - UniProt
- O95831
- 途径
- Apoptosis, Positive Regulation of Endopeptidase Activity, Cell RedoxHomeostasis, Smooth Muscle Cell Migration, Warburg Effect
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