GCLC 抗体 (N-Term)
(2 references)-
- 抗原 See all GCLC 抗体
- GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))
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抗原表位
- AA 7-24, N-Term
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适用
- 人, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This GCLC antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 原理
- Rabbit IgG polyclonal antibody for Glutamate--cysteine ligase catalytic subunit(GCLC) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
- 序列
- GSPLSWEETK RHADHVRR
- 交叉反应 （详细）
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Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities. - 产品特性
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Rabbit IgG polyclonal antibody for Glutamate--cysteine ligase catalytic subunit(GCLC) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: glutamate-cysteine ligase, catalytic subunit
Protein Name: Glutamate--cysteine ligase catalytic subunit - 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human GCLC(7-24aa GSPLSWEETKRHADHVRR), different from the related rat and mouse sequences by one amino acid.
- 亚型
- IgG
- Top Product
- Discover our top product GCLC Primary Antibody
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anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 528-637) antibody
GCLC 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3H1 unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (N-Term) antibodyGCLC 适用: 人, 小鼠, 大鼠, Cow, 斑马鱼, 兔, 犬, 豚鼠, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 1-30) antibodyGCLC 适用: 人 WB, IHC, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (N-Term) antibodyGCLC 适用: 人, 小鼠, 大鼠, Pig WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 437-637) antibodyGCLC 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 1-30), (N-Term) antibodyGCLC 适用: 人 WB, IF, FACS, IHC (p) 宿主: 兔 Polyclonal RB31687 unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 1-252) antibodyGCLC 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 435-636) antibodyGCLC 适用: 大鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (Middle Region) antibodyGCLC 适用: 人, 小鼠, 大鼠, Cow, 斑马鱼, 兔, 犬, 豚鼠, 马, Saccharomyces cerevisiae WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC) (AA 6-34), (N-Term) antibodyGCLC 适用: 人 WB, IF, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- 储存液
- Thimerosal (Merthiolate), Sodium azide
- 注意事项
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - 有效期
- 12 months
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: "Bone marrow mesenchymal stem cells protect against bleomycin-induced pulmonary fibrosis in rat by activating Nrf2 signaling." in: International journal of clinical and experimental pathology, Vol. 8, Issue 7, pp. 7752-61, (2016) (PubMed).
: "Electroacupuncture alleviates cerebral ischemia and reperfusion injury via modulation of the ERK1/2 signaling pathway." in: Neural regeneration research, Vol. 11, Issue 7, pp. 1090-8, (2016) (PubMed).
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: "Bone marrow mesenchymal stem cells protect against bleomycin-induced pulmonary fibrosis in rat by activating Nrf2 signaling." in: International journal of clinical and experimental pathology, Vol. 8, Issue 7, pp. 7752-61, (2016) (PubMed).
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- 抗原
- GCLC (Glutamate-Cysteine Ligase, Catalytic Subunit (GCLC))
- 别名
- GCLC (GCLC 产品)
- 别名
- GCLC antibody, gclc antibody, MGC146287 antibody, 2259 antibody, CG2259 antibody, DmGCLC antibody, DmGCS antibody, DmGCSh antibody, Dmel\\CG2259 antibody, GCL antibody, GCLc antibody, GCSh antibody, Gcs antibody, Gcsh antibody, DDBDRAFT_0186120 antibody, DDBDRAFT_0231403 antibody, DDB_0186120 antibody, DDB_0231403 antibody, GCS antibody, GLCL antibody, GLCLC antibody, D9Wsu168e antibody, GLCL-H antibody, Ggcs-hs antibody, Glclc antibody, cb1049 antibody, fe36e11 antibody, wu:fe36e11 antibody, glutamate-cysteine ligase catalytic subunit antibody, glutamate-cysteine ligase, catalytic subunit antibody, Glutamate-cysteine ligase catalytic subunit antibody, gamma glutamylcysteine synthetase antibody, glutamate-cysteine ligase, catalytic subunit L homeolog antibody, glutamate-cysteine ligase, catalytic subunit S homeolog antibody, gamma-glutamylcysteine synthetase antibody, GCLC antibody, gclc antibody, Gclc antibody, LbGCS antibody, gcsA antibody, gclc.L antibody, gclc.S antibody, GSH1 antibody
- 背景
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GCLC, Glutamate--cysteine ligase catalytic subunit, is an enzyme that in humans is encoded by the GCLC gene. Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. The gene encoding the catalytic subunit encodes a protein of 367 amino acids with a calculated molecular weight of 72.773 kDa and maps to chromosome 6p12.1. Deficiency of gamma-glutamylcysteine synthetase in human is associated with enzymopathic hemolytic anemia.
Synonyms: EC 6.3.2.2 antibody|Gamma ECS antibody|Gamma GCS antibody|Gamma glutamylcysteine synthetase antibody|Gamma-ECS antibody|Gamma-glutamylcysteine synthetase antibody|GCL antibody|GCLC antibody|GCLM antibody|GCS antibody|GCS Heavy Chain antibody|GLCL antibody|GLCLC antibody|GLCLR antibody|Glutamate cysteine ligase catalytic subunit antibody|Glutamate cysteine ligase(gamma glutamylcysteine synthetase) regulatory(30.8kD) antibody|Glutamate cysteine ligase catalytic subunit antibody|Glutamate cysteine ligase modifier subunit antibody|Glutamate cysteine ligase regulatory protein antibody|Glutamate cysteine ligase regulatory subunit antibody|Glutamate--cysteine ligase catalytic subunit antibody|GSC light chain antibody|GSH1_HUMAN antibody - UniProt
- P48506
- 途径
- Cell RedoxHomeostasis
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