GAD65 抗体 (N-Term)
(2 references)-
- 抗原 See all GAD65 (GAD2) 抗体
- GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))
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抗原表位
- AA 21-37, N-Term
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适用
- 人, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This GAD65 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 原理
- Rabbit IgG polyclonal antibody for Glutamate decarboxylase 2(GAD2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
- 序列
- ENPGTARAWC QVAQKFT
- 交叉反应 （详细）
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Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities. - 产品特性
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Rabbit IgG polyclonal antibody for Glutamate decarboxylase 2(GAD2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: glutamate decarboxylase 2(pancreatic islets and brain, 65 kDa)
Protein Name: Glutamate decarboxylase 2 - 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of human GAD65(21-37aa ENPGTARAWCQVAQKFT), identical to the related mouse and rat sequences.
- 亚型
- IgG
- Top Product
- Discover our top product GAD2 Primary Antibody
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anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 65-105) antibody
GAD2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 1-585) antibodyGAD2 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 188-374) antibodyGAD2 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 401-585) antibodyGAD2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (Internal Region) antibodyVerified GAD2 适用: 人 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (C-Term) antibodyGAD2 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 1-100) antibodyGAD2 适用: 人 WB, IHC, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 5D9G12 unconjugated
anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 131-164), (N-Term) antibodyGAD2 适用: 人, 大鼠, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 109-138) antibodyGAD2 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB19330 unconjugated
anti-Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2) (AA 501-585) antibodyGAD2 适用: 人, 大鼠, 小鸡 WB, ELISA, IHC (p), FACS, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- 储存液
- Thimerosal (Merthiolate), Sodium azide
- 注意事项
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - 有效期
- 12 months
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: "Acrylamide neurotoxicity on the cerebrum of weaning rats." in: Neural regeneration research, Vol. 10, Issue 6, pp. 938-43, (2015) (PubMed).
: "Effect of sub-acute exposure to acrylamide on GABAergic neurons and astrocytes in weaning rat cerebellum." in: Toxicology and industrial health, Vol. 28, Issue 1, pp. 10-20, (2012) (PubMed).
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: "Acrylamide neurotoxicity on the cerebrum of weaning rats." in: Neural regeneration research, Vol. 10, Issue 6, pp. 938-43, (2015) (PubMed).
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- 抗原
- GAD65 (GAD2) (Glutamate Decarboxylase 2 (Pancreatic Islets and Brain, 65kDa) (GAD2))
- 别名
- GAD2 (GAD2 产品)
- 别名
- GAD65 antibody, gad65 antibody, F12P19.12 antibody, F12P19_12 antibody, GLUTAMATE DECARBOXYLASE 2 antibody, glutamate decarboxylase 2 antibody, zgc:112198 antibody, 6330404F12Rik antibody, GAD(65) antibody, Gad-2 antibody, glutamate decarboxylase 2 antibody, glutamate decarboxylase 1 antibody, glutamic acid decarboxylase 2 antibody, GAD2 antibody, PTRG_06148 antibody, LOC9324534 antibody, Gad2 antibody, GAD1 antibody, gad2 antibody
- 背景
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Glutamate decarboxylase 2, also known as GAD65, is an enzyme that in humans is encoded by the GAD2 gene. This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Alternative splicing results in multiple transcript variants that encode the same protein.
Synonyms: 65 kDa glutamic acid decarboxylase antibody|DCE 2 antibody|DCE2 antibody|DCE2_HUMAN antibody|GAD 2 antibody|GAD 65 antibody|GAD-2 antibody|GAD-65 antibody|Gad2 antibody|Glutamate decarboxylase 2(pancreas) antibody|Glutamate Decarboxylase 2(pancreatic islets and brain 65 kDa) antibody|Glutamate decarboxylase 2 antibody|Glutamate Decarboxylase 65 antibody|Glutamate decarboxylase 65 kDa isoform antibody|Glutamic Acid Decarboxylase 2 antibody|Glutamic Acid Decarboxylase 65 antibody|MGC161605 antibody|MGC161607 antibody - UniProt
- Q05329
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