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- 抗原 See all SNAP25 抗体
- SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))
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抗原表位
- AA 186-200, C-Term
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适用
- 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This SNAP25 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 原理
- Rabbit IgG polyclonal antibody for Synaptosomal-associated protein 25(SNAP25) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
- 序列
- DSNKTRIDEA NQRAT
- 交叉反应 (详细)
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Predicted Cross Reactivity: human, mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities. - 产品特性
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Rabbit IgG polyclonal antibody for Synaptosomal-associated protein 25(SNAP25) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: synaptosomal-associated protein, 25 kDa
Protein Name: Synaptosomal-associated protein 25(SNAP-25) - 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human SNAP25(186-200aa DSNKTRIDEANQRAT), identical to the related rat and mouse sequences.
- 亚型
- IgG
- Top Product
- Discover our top product SNAP25 Primary Antibody
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- 储存液
- Thimerosal (Merthiolate), Sodium azide
- 注意事项
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - 有效期
- 12 months
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Synaptosomal-associated protein 25 may be an intervention target for improving sensory and locomotor functions after spinal cord contusion." in: Neural regeneration research, Vol. 12, Issue 6, pp. 969-976, (2017) (PubMed).
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Synaptosomal-associated protein 25 may be an intervention target for improving sensory and locomotor functions after spinal cord contusion." in: Neural regeneration research, Vol. 12, Issue 6, pp. 969-976, (2017) (PubMed).
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- 抗原
- SNAP25 (Synaptosomal-Associated Protein, 25kDa (SNAP25))
- 别名
- SNAP25 (SNAP25 产品)
- 别名
- RIC-4 antibody, RIC4 antibody, SEC9 antibody, SNAP antibody, SNAP-25 antibody, bA416N4.2 antibody, dJ1068F16.2 antibody, SNAP-25B antibody, SNAP-25a antibody, ric-4 antibody, ric4 antibody, sec9 antibody, snap-25 antibody, Bdr antibody, sp antibody, fj55h11 antibody, snap25 antibody, wu:fj55h11 antibody, Snap antibody, snap25.2 antibody, wu:fj33h05 antibody, synaptosome associated protein 25 antibody, synaptosome associated protein 25kDa S homeolog antibody, synaptosomal-associated protein, 25kDa antibody, synaptosomal-associated protein 25 antibody, synaptosome associated protein 25kDa antibody, synaptosome associated protein 25kDa L homeolog antibody, zgc:101731 antibody, synaptosomal-associated protein, 25b antibody, SNAP25 antibody, Snap25 antibody, snap25.S antibody, snap25 antibody, BACOIKO003_07 antibody, Tsp_10811 antibody, snap25.L antibody, zgc:101731 antibody, snap25b antibody
- 背景
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Synaptosome-associated protein of 25,000 daltons also known as SNAP-25 is a protein which in humans encodes a 25-kD protein of 206 amino acids. It was first investigated as a neuron-specific gene preferentially expressed in mouse hippocampus. The tSNARE(the target-membrane soluble NSF-attachment protein receptor, where NSF is N-ethylmaleimide-sensitive fusion protein) synaptosomal-associated protein of 25 kDa(SNAP-25) is expressed in pancreatic B-cells and its cleavage by botulinum neurotoxin E(BoNT/E) abolishes stimulated secretion of insulin. In the nervous system, two SNAP-25 isoforms(a and b) have been described, which are produced by alternative splicing. Nagy et al.(2004) identified mammalian Snap25a and Snap25b as targets of protein kinase A, a key regulator of neurosecretion that primes slowly releasable pools and readily releasable pools of secretory vesicles. SNAP-25 inhibits P/Q- and L-type voltage-gated calcium channels located presynaptically and interacts with the synaptotagmin C2B domain in Ca2+-independent fashion. In glutamatergic synapses SNAP-25 decreases the Ca2+ responsiveness, while it is naturally absent in GABAergic synapses.
Synonyms: bA416N4.2 antibody|Bdr antibody|dJ1068F16.2 antibody|FLJ23079 antibody|HGNC:11132 antibody|MGC105414 antibody|MGC139754 antibody|Resistance to inhibitors of cholinesterase 4 homolog antibody|RIC 4 antibody|RIC4 antibody|SEC 9 antibody|SEC9 antibody|SNAP 25 antibody|SNAP antibody|SNAP-25 antibody|SNAP-25B antibody|SNAP25 antibody|SNP 25 antibody|SNP25 antibody|SNP25_HUMAN antibody|sp antibody|SUP antibody|Super protein antibody|Synaptosomal associated 25 kDa protein antibody|Synaptosomal associated protein antibody|Synaptosomal Associated Protein 25 antibody|Synaptosomal associated protein 25 kDa antibody|Synaptosomal-associated 25 kDa protein antibody|Synaptosomal-associated protein 25 antibody|Synaptosomal-associated protein, 25-KD antibody - UniProt
- P60880
- 途径
- Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport
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