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NME2 抗体 (C-Term)

Name: NME2 抗体 (C-Term)
SKU: ABIN3044198
Availability: OutOfStock
Rating: 5 (1 reviews)

NME2 适用: 人, 小鼠, 大鼠 WB, IHC (p), ICC 宿主: 兔 Polyclonal unconjugated

Immunohistochemistry validation image for anti-Nucleoside diphosphate kinase B (NME2) (AA 137-152), (C-Term) antibody (ABIN3044198)

IHC

Western Blotting (WB) image for anti-Nucleoside diphosphate kinase B (NME2) (AA 137-152), (C-Term) antibody (ABIN3044198)

Anti-NME2 antibody, IHC(P) IHC(P): Human Intestinal Cancer Tissue

IHC

IHC

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IHC

5 images

(1 validation)

产品编号 ABIN3044198

发货至: 中国

Datasheet as PDF

抗原 See all NME2 抗体

NME2 (Nucleoside diphosphate kinase B (NME2))
抗原表位
15

8

8

5

2

2

1

1

1

1

1

1

1

1

1
AA 137-152, C-Term
适用
52

32

8

2

1

1

1
人, 小鼠, 大鼠
宿主
43

9

1
兔
Compatible Secondaries
克隆类型
44

9
多克隆
标记
29

3

3

3

2

2

1

1

1

1

1

1

1

1

1

1

1
This NME2 antibody is un-conjugated
应用范围
37

25

22

13

13

10

5

4

4

2

2

1

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)

原理

Rabbit IgG polyclonal antibody for Nucleoside diphosphate kinase B(NME2) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.

序列

EELVDYKSCA HDWVYE

交叉反应（详细）

No cross reactivity with other proteins.

产品特性

Rabbit IgG polyclonal antibody for Nucleoside diphosphate kinase B(NME2) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: NME/NM23 nucleoside diphosphate kinase 2
Protein Name: Nucleoside diphosphate kinase B(NDK B)

纯化方法

Immunogen affinity purified.

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human NME2(137-152aa EELVDYKSCAHDWVYE), different from the related mouse and rat sequences by one amino acid.

亚型

IgG

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anti-Nucleoside diphosphate kinase B (NME2) (AA 8-149) antibody
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anti-Nucleoside diphosphate kinase B (NME2) (AA 137-152) antibody
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anti-Nucleoside diphosphate kinase B (NME2) (AA 1-152) antibody
NME2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 4A7 unconjugated

anti-Nucleoside diphosphate kinase B (NME2) antibody
NME2 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Nucleoside diphosphate kinase B (NME2) (AA 25-54), (N-Term) antibody
NME2 适用: 人, 小鼠, 大鼠 WB, IHC (p), IF 宿主: 兔 Polyclonal RB3695 unconjugated

应用备注

WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

限制

仅限研究用
生效 #300030 (Immunohistochemistry)

by

Human Protein Atlas

No.

#300030

日期

2016.12.13

抗原

P18

Lot Number

Method validated

Immunohistochemistry

Positive Control

tonsil, urinary bladder

Negative Control

Notes

Passed. ABIN3044198 staining is consistent with staining pattern of other commercial P18/NME2 antibodies.

Validation Images

Staining of human tonsil (A.) and urinary bladder (B.) sections in a tissue microarray with ABIN3044198. See the protocol for detailed information.
Full Methods
Primary Antibody

ABIN3044198

Secondary Antibody

HRP polymer (ThermoFisher Scientific, TL-125-PH)

Full Protocol

Sample preparation:
Fixation and TMA preparation according to PMID: 22688270.
Cut 4µm TMA sections using a waterfall microtome (ThermoFisher Scientific, HM355S).
Dry sections ON at RT and then back them for 12-24h at 50°C.
Deparaffinization and rehydration in xylene and graded alcohol series:
xylene twice for 3min.
100% ethanol for 1min.
95% ethanol for 1min.
0.3% H₂O₂ in 95% ethanol for 5min to block endogenous peroxidase.
70% ethanol for 1min.
50% ethanol for 1min.
distilled H₂O for 2min.
Antigen Retrieval by Heat Induced Epitope Retrieval (HIER):
Boil the sections in Lab Vision Citrate buffer pH6.0 (ThermoFisher Scientific, TA-250-PM1X) for 4min at 125°C in a decloaking chamber (Biocare Medical).
Allow slides to cool down to 90°C in the decloaking chamber.
Immunostaining in a Lab Vision Autostainer 480 (ThermorFisher Scientific) at RT with 300µl of reagents for each step:
Rinse sections in Lab Vision wash buffer with extra tween added to a final concentration of 0.2% (ThermoFisher Scientific, TA-999-TT and TA-125-TW) wash buffer.
Block sections with Lab Vision Ultra V-Block (ThermoFisher Scientific, TA-125-UB) for 5min.
Rinse sections 2x in wash buffer.
Incubate sections with primary rabbit anti-P18/NME2 antibody (antibodies-online, ABIN3044198) diluted 1:25000 for 30min.
Rinse sections 3x in wash buffer.
Incubate sections with labeled HRP polymer (ThermoFisher Scientific, TL-125-PH) for 30min.
Rinse sections 2x in wash buffer.
Develop in DAB Quanto solution (ThermoFisher Scientific, TL-125-QHDX) for 5min.
Rinse sections in distilled H₂O.
Washing, counterstaining, and coverslipping in Autostainer XL (Leica Biosystems, ST5010) at RT:
Counterstaining in Mayer’s hematoxylin plus (HistoLab, 01820) for 7.5min.
Rinse sections in tap water for 5min.
Rinse sections in lithium carbonate water diluted 1:5 for 5min.
Dehydration in graded ethanol series and Neo-Clear (Merck Millipore, 109843) according to the manufacturer recommendations.
Automated mounting (Leica Biosystems, CV5030) of coverslip with Pertex (Histolab, 00871).
Microscopy:
Image acquisition on a Leica Aperio AT2 and Aperio Scanscope AT at 20x magnification.

Experimental Notes

ABIN3044198 shows staining in most tissues similar to alternative P18/NME2 antibodies. Determination of the appropriate dilution factor proved difficult.
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

储存液

Thimerosal (Merthiolate), Sodium azide

注意事项

This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

有效期

12 months
抗原

NME2 (Nucleoside diphosphate kinase B (NME2))

别名

NME2 (NME2 产品)

别名

NDKB antibody, NDPK-B antibody, NDPKB antibody, NM23-H2 antibody, NM23B antibody, PUF antibody, nm23-2 antibody, p18-12d antibody, nm23-M2 antibody, NM23-C2 antibody, NME/NM23 nucleoside diphosphate kinase 2 antibody, non-metastatic cells 2, protein (NM23B) expressed in antibody, NME2 antibody, Nme2 antibody

背景

NME2(NME/NM23 nucleoside diphosphate kinase 2) also called non-metastatic cells 2, protein(NM23B) expressed in, NM23-H2, NM23B or NDPKB, is identical to the beta subunit of human erythrocyte NDP kinase. Little to no expression was detected in other mouse tissues examined. Using Northern blot analysis, Masse et al.(2002) detected high expression of mouse Nme2, which they called nm23-M2, in heart, liver, and kidney, with intermediate expression in skeletal muscle. In situ hybridization of 15-day postcoitum mouse embryos showed ubiquitous Nme2 expression. The mouse and human NME2 genes contain 5 exons and span about 6.0 kb. The NME2 gene is mapped on 17q21.33. Srivastava et al.(2006) concluded that histidine phosphorylation regulates KCa3.1 channel activity and that NDPBK is critical to the channel activity and the activation of CD4 T cells.

Synonyms: C myc purine binding transcription factor PUF antibody|C myc transcription factor antibody|C-myc purine-binding transcription factor PUF antibody|Histidine protein kinase NDKB antibody|MGC111212 antibody|MGC2212 antibody|NDK B antibody|NDKB antibody|NDKB_HUMAN antibody|NDP kinase B antibody|NDPK B antibody|NDPKB antibody|NM23 H2 antibody|nm23-H2 antibody|NM23B antibody|nme2 antibody|Non metastatic cells 2, protein(NM23B) expressed in antibody|Nucleoside diphosphate kinase B antibody|Nucleotide diphosphate kinase B antibody|PUF antibody

UniProt

P22392