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GRP78 抗体 (C-Term)

HSPA5 适用: 人, 大鼠 WB, ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

Anti-GRP78 BiP antibody, Western blotting Lane 1: Rat Testis Tissue Lysate Lane 2: A549 Cell Lysate Lane 3: MCF-7 Cell Lysate Lane 4: HELA Cell Lysate

Anti-GRP78 BiP antibody, IHC(P) IHC(P): Human Lung Cancer Tissue

Anti-GRP78 BiP antibody, IHC(F) IHC(F): Rat Intestine Tissue

Anti-GRP78 BiP antibody, IHC(P) IHC(P): Rat Cerebellum Tissue

Anti-GRP78 BiP antibody, IHC(P) IHC(P): Rat Intestine Tissue

Anti-GRP78 BiP antibody, ICC ICC: HELA Cell

6 images

(4 references)

产品编号 ABIN3044191

发货至: 中国

Datasheet as PDF

抗原 See all GRP78 (HSPA5) 抗体

GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))
抗原表位
25

16

13

8

7

6

6

6

5

5

4

4

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 603-617, C-Term
适用
185

123

106

39

38

36

34

32

31

20

15

6

5

4

4

3

3

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1
人, 大鼠
宿主
131

90

9

3

1

1
兔
Compatible Secondaries
克隆类型
134

102
多克隆
标记
99

21

17

14

13

12

9

8

8

8

5

2

2

2

2

2

2

2

2

2

2

2
This GRP78 antibody is un-conjugated
应用范围
221

104

89

86

77

34

31

29

26

20

13

7

5

3

3

2

1
Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

原理

Rabbit IgG polyclonal antibody for 78 kDa glucose-regulated protein(HSPA5) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.

序列

EWLESHQDAD IEDFK

交叉反应（详细）

Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities.

产品特性

Rabbit IgG polyclonal antibody for 78 kDa glucose-regulated protein(HSPA5) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: heat shock 70 kDa protein 5(glucose-regulated protein, 78 kDa)
Protein Name: 78 kDa glucose-regulated protein

纯化方法

Immunogen affinity purified.

免疫原

A synthetic peptide corresponding to a sequence at the C-terminus of human GRP78 BiP(603-617aa EWLESHQDADIEDFK), identical to the related mouse and rat sequences.

亚型

IgG

Top Product

Discover our top product HSPA5 Primary Antibody
anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) (AA 550-650) antibody
HSPA5 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) antibody
HSPA5 适用: 大鼠 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) (Internal Region) antibody
HSPA5 适用: 人, 大鼠, 小鼠 WB, IF, ICC, IP, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) (AA 251-360) antibody
HSPA5 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) (AA 550-650) antibody
HSPA5 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) antibody (HRP)
HSPA5 适用: 大鼠 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal HRP

anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) antibody (FITC)
HSPA5 适用: 大鼠 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal FITC

anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) antibody (Alkaline Phosphatase (AP))
HSPA5 适用: 大鼠 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal Alkaline Phosphatase (AP)

anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) antibody (Biotin)
HSPA5 适用: 大鼠 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal Biotin

anti-Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5) antibody (PE)
HSPA5 适用: 大鼠 WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal PE

应用备注

WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human, Predicted Species: Mouse, Rat
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

储存液

Thimerosal (Merthiolate), Sodium azide

注意事项

This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

有效期

12 months
Wang, Wang, Huang, Yang, Zhao, Wei, Zhao: "Erratum to: Ibutilide protects against cardiomyocytes injury via inhibiting endoplasmic reticulum and mitochondrial stress pathways." in: Heart and vessels, Vol. 32, Issue 2, pp. 216, (2016) (PubMed).

Wu, Dong, Li: "Effects of miRNA-455 on cardiac hypertrophy induced by pressure overload." in: International journal of molecular medicine, Vol. 35, Issue 4, pp. 893-900, (2015) (PubMed).

Li, Zhao, Xing, Sun: "Ulinastatin suppresses endoplasmic reticulum stress and apoptosis in the hippocampus of rats with acute paraquat poisoning." in: Neural regeneration research, Vol. 10, Issue 3, pp. 467-72, (2015) (PubMed).

Ding, Zou, Li, Tian, Abdelalim, Du, She, Wang, Tan, Wang, Chen, Lv, Chang: "Study of histopathological and molecular changes of rat kidney under simulated weightlessness and resistance training protective effect." in: PLoS ONE, Vol. 6, Issue 5, pp. e20008, (2011) (PubMed).
抗原

GRP78 (HSPA5) (Heat Shock 70kDa Protein 5 (Glucose-Regulated Protein, 78kDa) (HSPA5))

别名

HSPA5 (HSPA5 产品)

别名

GRP78 antibody, BiP antibody, GRP-78 antibody, grp78 antibody, hspa5a antibody, BIP antibody, MIF2 antibody, AL022860 antibody, AU019543 antibody, Bip antibody, D2Wsu141e antibody, D2Wsu17e antibody, Grp78 antibody, Hsce70 antibody, SEZ-7 antibody, Sez7 antibody, baffled antibody, mBiP antibody, cb865 antibody, fb60h09 antibody, fi36d04 antibody, wu:fb60h09 antibody, wu:fi36d04 antibody, zgc:55994 antibody, zgc:77606 antibody, 78 kDa glucose-regulated protein antibody, heat shock protein family A (Hsp70) member 5 antibody, BiP/GRP78 antibody, glucose-regulated protein 78 antibody, putative glucose-regulated protein 78 antibody, Hsp70 family ATPase KAR2 antibody, heat shock protein family A (Hsp70) member 5 S homeolog antibody, heat shock 70 kDa protein 5a antibody, heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) antibody, heat shock protein 5 antibody, heat shock protein family A member 5 antibody, CpipJ_CPIJ003550 antibody, HSPA5 antibody, grp78 antibody, LOC100533358 antibody, BiP/grp78 antibody, Tc00.1047053506585.40 antibody, Tb11.02.5450 antibody, Tb11.02.5500 antibody, LMJF_28_1200 antibody, KAR2 antibody, LOC100135840 antibody, hspa5.S antibody, hspa5 antibody, Hspa5 antibody

背景

HSPA5(heat shock 70 kDa protein 5) also known as glucose-regulated protein, 78kD(GRP78) or BiP, is a member of the heat-shock protein-70(HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum. BiP is also an essential component of the translocation machinery, as well as playing a role in retrograde transport across the ER membrane of aberrant proteins destined for degradation by the proteasome. The HSPA5 gene is mapped on 9q33.3. Shen et al.(2002) concluded that HSPA5 retains ATF6 in the ER by inhibiting its Golgi localization signals and that dissociation of HSPA5 during ER stress allows ATF6 to be transported to the Golgi. The findings of Shen et al.(2002) demonstrated that HSPA5 is a key element in sensing the folding capacity within the ER.

Synonyms: 78 kDa glucose regulated protein antibody|78 kDa glucose-regulated protein antibody|AL022860 antibody|AU019543 antibody|BIP antibody|D2Wsu141e antibody|D2Wsu17e antibody|Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody|Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody|FLJ26106 antibody|Glucose Regulated Protein 78 kDa antibody|GRP 78 antibody|GRP-78 antibody|GRP78 antibody|GRP78_HUMAN antibody|Heat shock 70 kDa protein 5 antibody|Heat Shock 70 kDa Protein 5 antibody|Hsce70 antibody|HSPA 5 antibody|HSPA5 antibody|Immunoglobulin Heavy Chain Binding Protein antibody|Immunoglobulin heavy chain-binding protein antibody|mBiP antibody|MIF2 antibody|Sez7 antibody

UniProt

P11021

途径

Thyroid Hormone Synthesis, ER-Nucleus Signaling

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