CYP2E1 抗体 (N-Term)
(3 references)-
- 抗原 See all CYP2E1 抗体
- CYP2E1 (Cytochrome P450, Family 2, Subfamily E, Polypeptide 1 (CYP2E1))
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抗原表位
- AA 94-112, N-Term
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适用
- 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CYP2E1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 原理
- Rabbit IgG polyclonal antibody for Cytochrome P450 2E1(CYP2E1) detection. Tested with WB, IHC-P in Mouse,Rat.
- 序列
- KNEFSGRGDI PVFQEYKNK
- 交叉反应 （详细）
- No cross reactivity with other proteins.
- 产品特性
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Rabbit IgG polyclonal antibody for Cytochrome P450 2E1(CYP2E1) detection. Tested with WB, IHC-P in Mouse,Rat.
Gene Name: cytochrome P450, family 2, subfamily E, polypeptide 1
Protein Name: Cytochrome P450 2E1 - 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence at the N-terminus of mouse CYP2E1(94-112aa KNEFSGRGDIPVFQEYKNK), identical to the related rat sequence.
- 亚型
- IgG
- Top Product
- Discover our top product CYP2E1 Primary Antibody
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anti-Cytochrome P450, Family 2, Subfamily E, Polypeptide 1 (CYP2E1) (AA 34-273) antibody
CYP2E1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome P450, Family 2, Subfamily E, Polypeptide 1 (CYP2E1) (AA 234-493) antibodyCYP2E1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome P450, Family 2, Subfamily E, Polypeptide 1 (CYP2E1) (AA 1-310) antibodyCYP2E1 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome P450, Family 2, Subfamily E, Polypeptide 1 (CYP2E1) (AA 1-493) antibodyCYP2E1 适用: 人 WB, IF, IHC (p) 宿主: 小鼠 Polyclonal unconjugated
anti-Cytochrome P450, Family 2, Subfamily E, Polypeptide 1 (CYP2E1) (Internal Region) antibodyCYP2E1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome P450, Family 2, Subfamily E, Polypeptide 1 (CYP2E1) (AA 402-429) antibodyCYP2E1 适用: 人 WB, IHC, ELISA, FACS 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome P450, Family 2, Subfamily E, Polypeptide 1 (CYP2E1) (AA 401-493) antibodyCYP2E1 适用: 人, 大鼠, Pig WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome P450, Family 2, Subfamily E, Polypeptide 1 (CYP2E1) (C-Term) antibodyCYP2E1 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome P450, Family 2, Subfamily E, Polypeptide 1 (CYP2E1) antibodyCYP2E1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Cytochrome P450, Family 2, Subfamily E, Polypeptide 1 (CYP2E1) (AA 1-493) antibodyCYP2E1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Mouse, Rat, The detection limit for CYP2E1 is approximately 1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- 储存液
- Thimerosal (Merthiolate), Sodium azide
- 注意事项
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - 有效期
- 12 months
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: "Wuzhi tablet (Schisandra Sphenanthera extract) protects against acetaminophen-induced hepatotoxicity by inhibition of CYP-mediated bioactivation and regulation of NRF2-ARE and p53/p21 pathways." in: Drug metabolism and disposition: the biological fate of chemicals, Vol. 42, Issue 12, pp. 1982-90, (2014) (PubMed).
: "Schisandrol B protects against acetaminophen-induced hepatotoxicity by inhibition of CYP-mediated bioactivation and regulation of liver regeneration." in: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 143, Issue 1, pp. 107-15, (2014) (PubMed).
: "Protective effect of tea polyphenols against paracetamol-induced hepatotoxicity in mice is significantly correlated with cytochrome P450 suppression." in: World journal of gastroenterology, Vol. 15, Issue 15, pp. 1829-35, (2009) (PubMed).
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: "Wuzhi tablet (Schisandra Sphenanthera extract) protects against acetaminophen-induced hepatotoxicity by inhibition of CYP-mediated bioactivation and regulation of NRF2-ARE and p53/p21 pathways." in: Drug metabolism and disposition: the biological fate of chemicals, Vol. 42, Issue 12, pp. 1982-90, (2014) (PubMed).
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- 抗原
- CYP2E1 (Cytochrome P450, Family 2, Subfamily E, Polypeptide 1 (CYP2E1))
- 别名
- CYP2E1 (CYP2E1 产品)
- 别名
- CYP2E1 antibody, CYP2E antibody, CYPIIE1 antibody, CPE1 antibody, P450-J antibody, P450C2E antibody, Cyp2e antibody, cytochrome P450, family 2, subfamily E, polypeptide 1 antibody, cytochrome P450 2E1 antibody, cytochrome P450 2E1-like antibody, cytochrome P450 family 2 subfamily E member 1 antibody, cytochrome P450, family 2, subfamily e, polypeptide 1 antibody, CYP2E1 antibody, PTRG_07411 antibody, LOC100342572 antibody, LOC100727914 antibody, LOC101843716 antibody, Cyp2e1 antibody
- 背景
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CYP2E1, also known as P450IIE1, is a member of the P450IIE subfamily which is ethanol-inducible. It has at least 1 gene which is mapped to 10q24.3-qter, and a second is likely in rat and in man. Both the rat and human proteins encoded by this gene contain 493 amino acids and calculated molecular masses of 56,634 and 56,916 daltons, respectively. In addition, genetic polymorphisms in the 5-prime flanking region of the human P450IIE1 gene affected its binding of transacting factor and changed its transcriptional regulation, which may lead to interindividual differences of microsomal drug oxidation activity. P450IIE1 is an important enzyme for the catalysis of the conversion of ethanol to acetaldehyde and to acetate in humans,and it is also involved in the metabolism of nitrosamines. Due to the possible correlation of P450IIE1 genes with malignancy, clinical studies of RFLP patterns of these genes in cancer may be useful.
Synonyms: 4-nitrophenol 2-hydroxylase antibody|CP2E1_HUMAN antibody|CPE 1 antibody|CPE1 antibody|CYP2E antibody|CYP2E1 antibody|CYPIIE1 antibody|Cytochrome P450 2E1 antibody|Cytochrome P450 family 2 subfamily E polypeptide 1 antibody|Cytochrome P450 isozyme 3A antibody|Cytochrome P450 LM3A antibody|Cytochrome P450 subfamily IIE(ethanol inducible) polypeptide 1 antibody|Cytochrome P450 subfamily IIE polypeptide 1 antibody|Cytochrome P450, family 2, subfamily e, polypeptide 1 antibody|cytochrome P450, subfamily IIE(ethanol-inducible), polypeptide 1 antibody|Cytochrome P450, subfamily IIE antibody|Cytochrome P450-ALC antibody|Cytochrome P450-J antibody|Cytochrome P450RLM6 antibody|EC 1.14.13.- antibody|EC 1.14.13.n7 antibody|Ethanol-inducible P450 antibody|Flavoprotein linked monooxygenase antibody|Flavoprotein-linked monooxygenase antibody|MGC133529 antibody|Microsomal monooxygenase antibody|OTTHUMP00000020813 antibody|OTTHUMP00000046571 antibody|P450 ALC antibody|p450 antibody|P450 J antibody|P450-J antibody|P450C2E antibody|P450J antibody|P450RLM6 antibody|Xenobiotic monooxygenase antibody - UniProt
- Q05421
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