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EIF2S1 抗体 (Middle Region)

EIF2S1 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN3044058
发货至: 中国
  • 抗原 See all EIF2S1 抗体
    EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))
    抗原表位
    • 19
    • 9
    • 8
    • 8
    • 5
    • 5
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 123-137, Middle Region
    适用
    • 70
    • 42
    • 32
    • 9
    • 8
    • 7
    • 7
    • 6
    • 6
    • 6
    • 5
    • 5
    • 4
    • 3
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    • 1
    • 1
    • 1
    • 1
    宿主
    • 74
    • 11
    • 2
    克隆类型
    • 70
    • 17
    多克隆
    标记
    • 53
    • 5
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This EIF2S1 antibody is un-conjugated
    应用范围
    • 58
    • 37
    • 32
    • 30
    • 18
    • 13
    • 13
    • 12
    • 8
    • 6
    • 3
    • 2
    • 1
    • 1
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    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    原理
    Rabbit IgG polyclonal antibody for Eukaryotic translation initiation factor 2 subunit 1(EIF2S1) detection. Tested with WB, IHC-P in Human.
    序列
    KDEQLESLFQ RTAWV
    交叉反应 (详细)
    No cross reactivity with other proteins.
    产品特性
    Rabbit IgG polyclonal antibody for Eukaryotic translation initiation factor 2 subunit 1(EIF2S1) detection. Tested with WB, IHC-P in Human.
    Gene Name: eukaryotic translation initiation factor 2, subunit 1 alpha, 35 kDa
    Protein Name: Eukaryotic translation initiation factor 2 subunit 1
    纯化方法
    Immunogen affinity purified.
    免疫原
    A synthetic peptide corresponding to a sequence in the middle region of human EIF2S1(123-137aa KDEQLESLFQRTAWV).
    亚型
    IgG
    Top Product
    Discover our top product EIF2S1 Primary Antibody
  • 应用备注
    WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for EIF2S1 is approximately 0.5 ng/lane under reducing conditions.
    IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
    Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users.
    说明

    Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式
    Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
    浓度
    500 μg/mL
    缓冲液
    Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
    储存液
    Thimerosal (Merthiolate), Sodium azide
    注意事项
    This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    At -20°C for one year. After reconstitution, at 4°C for one month.
    It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
    有效期
    12 months
  • 抗原
    EIF2S1 (Eukaryotic Translation Initiation Factor 2 Subunit 1 (EIF2S1))
    别名
    EIF2S1 (EIF2S1 产品)
    背景
    EIF2S1(Eukaryotic Translation Initiation Factor 2, Subunit 1), also called EIF2-alpha, is a protein that in humans is encoded by the EIF2S1 gene. Hartz(2010) mapped the EIF2S1 gene to chromosome 14q23.3 based on an alignment of the EIF2S1 sequence with the genomic sequence(GRCh37). Ernst et al.(1987) stated that protein synthesis is inhibited due to phosphorylation of Eif2-alpha in hemin-deprived rabbit reticulocyte lysates. HeLa cells subjected to heat shock, serum deprivation, or interferon treatment followed by virus infection also show a correlation between EIF2-alpha phosphorylation and translational repression. Jacob et al.(1989) found that the alpha-Pal transcription factor bound to 2 palindromic sites within the EIF2-alpha promoter and was essential for transcription of the EIF2-alpha gene.

    Synonyms: EIF 2 alpha antibody|EIF 2 antibody|EIF 2A antibody|EIF 2alpha antibody|eIF-2-alpha antibody|eIF-2A antibody|EIF-2alpha antibody|EIF2 alpha antibody|EIF2 antibody|EIF2A antibody|EIF2S1 antibody|Eukaryotic translation initiation factor 2 subunit 1 alpha 35 kDa antibody|Eukaryotic translation initiation factor 2 subunit 1 alpha antibody|Eukaryotic translation initiation factor 2 subunit 1 antibody|Eukaryotic translation initiation factor 2 subunit alpha antibody|IF2A_HUMAN antibody
    UniProt
    P05198
    途径
    Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C
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