NOX5 抗体 (C-Term)
(1 reference)-
- 抗原 See all NOX5 抗体
- NOX5 (NADPH Oxidase 5 (NOX5))
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抗原表位
- AA 747-765, C-Term
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This NOX5 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 原理
- Rabbit IgG polyclonal antibody for NADPH oxidase 5(NOX5) detection. Tested with WB, IHC-P, ICC in Human.
- 序列
- KVLKGHCEKF GFRFFQENF
- 交叉反应 （详细）
- No cross reactivity with other proteins.
- 产品特性
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Rabbit IgG polyclonal antibody for NADPH oxidase 5(NOX5) detection. Tested with WB, IHC-P, ICC in Human.
Gene Name: NADPH oxidase, EF-hand calcium binding domain 5
Protein Name: NADPH oxidase 5 - 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human NOX5(747-765aa KVLKGHCEKFGFRFFQENF).
- 亚型
- IgG
- Top Product
- Discover our top product NOX5 Primary Antibody
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anti-NADPH Oxidase 5 (NOX5) (AA 456-765) antibody
NOX5 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-NADPH Oxidase 5 (NOX5) (AA 661-765) antibodyNOX5 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-NADPH Oxidase 5 (NOX5) (AA 501-600) antibodyNOX5 适用: 人 WB, ELISA, IF (cc), IF (p), ICC, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-NADPH Oxidase 5 (NOX5) (AA 661-765) antibodyNOX5 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-NADPH Oxidase 5 (NOX5) (C-Term) antibodyNOX5 适用: 人 WB, ELISA, ICC, IF 宿主: 兔 Polyclonal unconjugated
anti-NADPH Oxidase 5 (NOX5) (Internal Region) antibodyVerified NOX5 适用: 人 WB, ELISA, IF 宿主: 山羊 Polyclonal unconjugated
anti-NADPH Oxidase 5 (NOX5) antibodyNOX5 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-NADPH Oxidase 5 (NOX5) (C-Term) antibodyNOX5 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-NADPH Oxidase 5 (NOX5) (AA 680-709) antibodyNOX5 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- 储存液
- Thimerosal (Merthiolate), Sodium azide
- 注意事项
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - 有效期
- 12 months
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: "CRISPR-Cas9 Mediated NOX4 Knockout Inhibits Cell Proliferation and Invasion in HeLa Cells." in: PLoS ONE, Vol. 12, Issue 1, pp. e0170327, (2017) (PubMed).
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: "CRISPR-Cas9 Mediated NOX4 Knockout Inhibits Cell Proliferation and Invasion in HeLa Cells." in: PLoS ONE, Vol. 12, Issue 1, pp. e0170327, (2017) (PubMed).
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- 抗原
- NOX5 (NADPH Oxidase 5 (NOX5))
- 别名
- NOX5 (NOX5 产品)
- 别名
- NADPH oxidase 5 antibody, NOX5 antibody
- 背景
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NOX5(Nadph Oxidase 5), also known as NOX5A or NOX5B, is a protein which in humans is encoded by the NOX5 gene. The International Radiation Hybrid Mapping Consortium mapped the NOX5 gene to chromosome 15. NOX5 is a novel NADPH oxidase that generates superoxide and functions as an H+ channel in a Ca2+-dependent manner. Banfi et al.(2001) found that, when heterologously expressed, NOX5 was quiescent in unstimulated cells. However, in response to elevations of the cytosolic Ca2+ concentration, it generated large amounts of superoxide. Using RT-PCR and Southern and Western blot analyses, Kamiguti et al.(2005) identified NOX5 as a flavin-containing Ca2+-dependent oxidase present in hairy leukemic cells(HC), but not normal B cells.
Synonyms: MGC149776 antibody|MGC149777 antibody|NADPH oxidase 5 antibody|NADPH oxidase EFhand calcium binding domain 5 antibody|NOX5 antibody|NOX5_HUMAN antibody - 途径
- Proton Transport
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