Immunohistochemistry on Paraffin Sections (20 μg/mL). Immunofluorescence. Western Blot(1/500-1/2000). ELISA (1/5000-1/25000). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C
储存方法
Store the antibody undiluted (in aliquots) at-20 °C. Dilute only prior to immediate use.
抗原
HMGN1
(High Mobility Group Nucleosome Binding Domain 1 (HMGN1))
HMG14 antibody, hmg14 antibody, hmgn1 antibody, hmgn1b antibody, HMG-14 antibody, Hmg14 antibody, high mobility group nucleosome binding domain 1 antibody, high mobility group nucleosome binding domain 1 S homeolog antibody, high mobility group nucleosomal binding domain 1 antibody, non-histone chromosomal protein HMG-14 antibody, HMGN1 antibody, hmgn1.S antibody, hmgn1 antibody, Hmgn1 antibody, LOC785479 antibody
背景
HMGNs are proteins that bind chromatin effectively reducing the compaction of the chromatin fiber and enhancing access to DNA regulatory sequences. Members of this family have a conserved chromatin binding domain which is phosphorylated during mitosis. The sequence immunized is conserved in several species. As such, this reagent is designed as a universal reagent for the detection of all phosphorylated HMGN proteins. The High Mobility Group (HMG) proteins were originally isolated from mammalian cells and were named according to their electrophoretic mobility in polyacrylamide gels. HMGs were arbitrarily classed as a specific type of nonhistone proteins based on the observation that they are ubiquitous to mammalian cells, that they share certain physical properties, and that they are associated with isolated chromatin. HMG proteins and are now subdivided into 3 families: the HMGB (formerly HMG-1/-2) family, the HMGN (formerly HMG-14/-17) family, and the HMGA (formerly HMG-I/Y/C) family. Each HMG family has a characteristic functional sequence motif. The functional motif of the HMGB family is called the "HMG-box," that of the HMGN family, the "nucleosomal binding domain," and that of the HMGA family, the "AT-hook." The functional motifs characteristic of these canonical HMGs are widespread among nuclear proteins in a variety of organisms. Proteins containing any of these functional motifs embedded in their sequence are known as "HMG motif proteins."Synonyms: HMG-14, HMG14, High mobility group nucleosome-binding domain-containing protein 1, Non-histone chromosomal protein HMG-14