The stated application concentrations are suggested starting amounts. Titration of the IkB beta antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
限制
仅限研究用
缓冲液
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
储存条件
-20 °C
储存方法
After reconstitution, the IkB beta antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
NF-kappa-B inhibitor beta, also known as IKBB or TRIP9, is a protein that in humans is encoded by the NFKBIB gene. The protein encoded by this gene belongs to the < a href=../search_result.php?search_txt=nfkb>NF-kappa-B inhibitor family, which inhibit NF-kappa-B by complexing with it and therebye trapping it in the cytoplasm. It has been found that in vivo, IkB beta serves both to inhibit and to facilitate the inflammatory response. Its degradation releases NF-kappa-B dimers, which upregulate proinflammatory target genes such as < a href=../search_result.php?search_txt=tnf alpha>TNF-alpha. Surprisingly, absence of IkB beta results in a dramatic reduction of TNF-alpha in response to lipopolysaccharide, even though activation of NF-kappa-B is normal.