The stated application concentrations are suggested starting amounts. Titration of the NMI antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
限制
仅限研究用
缓冲液
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
储存条件
-20 °C
储存方法
After reconstitution, the NMI antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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背景
NMYC interactor (NMI) is a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. NMI also interacts with all < a href=../search_result.php?search_txt=stat>STATs except < a href=../search_result.php?search_txt=stat2>STAT2 and augments STAT-mediated transcription in response to cytokines < a href=../search_result.php?search_txt=il2>IL2 and < a href=../search_result.php?search_txt=ifn gamma>IFN-gamma. mRNA has low expression levels in all human fetal and adult tissues tested except brain and has high expression in cancer cell line-myeloid leukemias.