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MYL9 抗体 (AA 12-27)

MYL9 适用: 人, 小鼠, 大鼠, Avian, 哺乳动物 WB, IHC (p), IP, EIA 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN303105
发货至: 中国
  • 抗原 See all MYL9 抗体
    MYL9 (Myosin Regulatory Light Chain 2, Smooth Muscle Isoform (MYL9))
    抗原表位
    • 16
    • 15
    • 11
    • 9
    • 7
    • 6
    • 5
    • 5
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 12-27
    适用
    • 79
    • 36
    • 30
    • 5
    • 4
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    人, 小鼠, 大鼠, Avian, 哺乳动物
    宿主
    • 83
    • 2
    克隆类型
    • 82
    • 3
    多克隆
    标记
    • 38
    • 7
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This MYL9 antibody is un-conjugated
    应用范围
    • 73
    • 37
    • 32
    • 26
    • 26
    • 13
    • 12
    • 6
    • 6
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
    特异性
    Amino acids 12-27 of Human Myosin light chain protein.
    交叉反应 (详细)
    Species reactivity (expected):Avian, Mammal, Mouse and Rat.
    Species reactivity (tested):Human.
    纯化方法
    Immunoaffinity Chromatography.
    免疫原
    Synthetic peptide - KLH conjugated.
    Top Product
    Discover our top product MYL9 Primary Antibody
  • 应用备注
    ELISA (1/5000-1/20000). Western Blot (1/500-1/2000). Immunoprecipitation. Immunohistochemistry on Paraffin Sections (2.5 μg/mL). This antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody LS-D1, followed by Alkalinephosphatase-streptavidin and chromogen.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 浓度
    0.71 mg/mL
    缓冲液
    0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preserevative.
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
  • 抗原
    MYL9 (Myosin Regulatory Light Chain 2, Smooth Muscle Isoform (MYL9))
    别名
    MYL9 (MYL9 产品)
    别名
    LC20 antibody, MLC-2C antibody, MLC2 antibody, MRLC1 antibody, MYRL2 antibody, AI327049 antibody, MLC20 antibody, Mylc2c antibody, RLC-C antibody, myosin antibody, regulatory antibody, MRCL3 antibody, MRLC2 antibody, MYL9 antibody, zgc:103467 antibody, myl9 antibody, zgc:77916 antibody, myosin light chain 9 antibody, myosin, light polypeptide 9, regulatory antibody, myosin light chain 9 L homeolog antibody, myosin, light chain 9, regulatory antibody, myosin, light chain 12A, regulatory, non-sarcomeric antibody, myosin, light chain 9a, regulatory antibody, myosin, light chain 9b, regulatory antibody, MYL9 antibody, Myl9 antibody, myl9.L antibody, MYL12A antibody, myl9a antibody, myl9b antibody
    背景
    Myosin is the major component of thick muscle filaments, and is a long asymmetric molecule containing a globular head and a long tail. The molecule consists of two heavy chains each ~200,000 daltons, and four light chains each ~16,000 - 21,000 daltons. Activation of smooth and cardiac muscle primarily involves pathways that increase calcium and myosin phosphorylation resulting in contraction. Myosin light chain phosphatase acts to regulate muscle contraction by dephosphorylating activated myosin light chain. The selected peptide sequence used to generate the polyclonal antibody is located near the amino terminal end of the polypeptide corresponding to the smooth/non-muscle form of myosin regulatory light chain found in cardiac myocytes in addition to smooth and non-muscle cells. This sequence differs from that of the sarcomeric/ cardiac form of myosin regulatory light chain that has a different sequence around the phosphorylation site. Human, Mouse and Rat have almost identical sequences.Synonyms: LC20, MLC-2C, MLC2, MRLC1, MYRL2, Myosin RLC, Myosin regulatory light chain 2, Myosin regulatory light chain MRLC1, Myosin regulatory light polypeptide 9, smooth muscle
    基因ID
    10398
    NCBI登录号
    NP_006088
    UniProt
    P24844
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