TAG-72 抗体
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- 抗原 See all TAG-72 抗体
- TAG-72 (Temporarily Assigned Gene Name Family Member (TAG-72))
- 适用
- 人, 大鼠, Cow, 犬, Hamster
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This TAG-72 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 产品特性
- This antibody recognizes an oncofetal antigen of 220 kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This antibody defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80 % sensitivity and 93 % specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of TAG-72 Ab with a GCDFP-15 Ab is valuable in the diagnosis of apocrine carcinoma.
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- The membrane-enriched fraction of a human breast carcinoma liver metastasis was used as the immunogen for this TAG-72 antibody.
- 克隆位点
- B72-3
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product TAG-72 Primary Antibody
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anti-Temporarily Assigned Gene Name Family Member (TAG-72) antibody
TAG-72 适用: 人 IHC (p), WB 宿主: 小鼠 Monoclonal SPM148 unconjugated
anti-Temporarily Assigned Gene Name Family Member (TAG-72) antibodyTAG-72 适用: 人, 大鼠, Cow, 犬 FACS, IF, IHC (p), WB 宿主: 小鼠 Monoclonal CC49 unconjugated
anti-Temporarily Assigned Gene Name Family Member (TAG-72) antibodyTAG-72 适用: 人 FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal TAGP-4 unconjugated
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- 应用备注
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The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the TAG-72 antibody to be titered up or down for optimal performance.
1. No pretreatment is required for staining of formalin-fixed, paraffin-embedded tissues.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 1-2 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- 1 mg/mL in 1X PBS, BSA free, sodium azide free
- 储存液
- Azide free
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the TAG-72 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- TAG-72 (Temporarily Assigned Gene Name Family Member (TAG-72))
- 别名
- TAG-72 (TAG-72 产品)
- 别名
- mRNA cap guanine-N7 methyltransferase antibody, tag-72 antibody
- 背景
- This antibody recognizes an oncofetal antigen of 220 kDa, identified as a tumor-associated glycoprotein (TAG-72) with properties of a mucin. This antibody defines the mucin-carried sialylated-Tn epitope. TAG-72 is usually expressed by adenocarcinomas, but is negative in mesotheliomas. Studies have reported that this antibody has 80 % sensitivity and 93 % specificity for pulmonary adenocarcinoma. Therefore, TAG-72 is a useful marker to distinguish between mesothelioma and adenocarcinoma. However, false positive reactions can occur so results must be interpreted with the utmost caution. This antibody may be useful in the differentiation of non-small cell carcinomas from small cell carcinomas of the lung. The combined use of TAG-72 Ab with a GCDFP-15 Ab is valuable in the diagnosis of apocrine carcinoma.
- 基因ID
- 182875
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