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- 抗原 See all IgM products
- IgM
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Protein G
- 免疫原
- Human Mu heavy chain was used as the immunogen for this IgM antibody.
- 克隆位点
- MuHC2
- 亚型
- IgG1 kappa
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Mouse anti-Human IgM antibody (HRP)
ELISA, WB Monoclonal CH2 IgG1 HRP
Goat anti-Human IgM (Heavy & Light Chain) antibody - PreadsorbedELISA, IHC, WB Polyclonal IgG unconjugated
Goat anti-Human IgM (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Goat anti-Human IgM (Heavy & Light Chain) antibody (Alkaline Phosphatase (AP)) - PreadsorbedELISA, IHC, WB Polyclonal IgG Alkaline Phosphatase (AP)
Mouse anti-Human IgM (Fc5mu Region) antibodyELISA, WB Monoclonal 22G1-H8 IgG2b kappa unconjugated
Mouse anti-Human IgM (Heavy Chain) antibody (HRP)ELISA, IHC, WB Monoclonal SA-DA4 IgG1 HRP
Mouse anti-Human IgM (Heavy Chain) antibody (Alkaline Phosphatase (AP))ELISA, IHC, WB Monoclonal SA-DA4 IgG1 Alkaline Phosphatase (AP)
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- 应用备注
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Due to differences in protocols and secondaries, the IgM antibody may need to be titered for optimal performance.
1. FFPE staining requires boiling sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunofluorescence: 0.5-1 μg/mL,Western blot: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) - 限制
- 仅限研究用
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- 浓度
- 0.2 mg/mL
- 缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the IgM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- IgM
- Abstract
- IgM 产品
- 物质类
- Antibody
- 背景
- IgM is the first antibody generated in an immune response to an antigen. It is generally a pentamer with each of the five immunoglobulins linked together with disulfide bonds. In its pentamer form, it has a molecular mass of 970 kDa and 10 antigen binding sites (due to the large size of most antigens, not all binding sites can be filled simultaneously. IgM antibodies account for approximately 5 % -10 % of all the antibody in the body.
- 基因ID
- 3507
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