VCP 抗体
(2 references)-
- 抗原 See all VCP 抗体
- VCP (Valosin Containing Protein (VCP))
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适用
- 人, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This VCP antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunocytochemistry (ICC), Flow Cytometry (FACS)
- 特异性
- The antibody Hs-14 reacts with VCP (valosin-containing protein) a 220 kDa intra-acrosomal protein.
- 交叉反应 （详细）
- Mouse, Human
- 纯化方法
- Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
- 纯度
- > 95 % (by SDS-PAGE)
- 免疫原
- Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
- 克隆位点
- Hs-14
- 亚型
- IgM
- Top Product
- Discover our top product VCP Primary Antibody
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anti-Valosin Containing Protein (VCP) (N-Term) antibody
VCP 适用: 人 WB, IF, IP, IHC (p), ICC, IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-Valosin Containing Protein (VCP) (C-Term) antibodyKO Validated VCP 适用: 人 WB, IF, IP, IHC (p), ICC, IHC (fro), IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-Valosin Containing Protein (VCP) (AA 125-371) antibodyVCP 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Valosin Containing Protein (VCP) (AA 1-588) antibodyVCP 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 4A8 unconjugated
anti-Valosin Containing Protein (VCP) (AA 647-806) antibodyVCP 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Valosin Containing Protein (VCP) antibodyVCP 适用: 人 WB, ELISA, IHC, IF, IP 宿主: 兔 Monoclonal 5H12 unconjugated Recombinant Antibody
anti-Valosin Containing Protein (VCP) (AA 732-760), (C-Term) antibodyVCP 适用: 人, 小鼠, 大鼠 WB, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Valosin Containing Protein (VCP) (AA 749-766), (C-Term) antibodyVCP 适用: 人, 大鼠 WB, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Valosin Containing Protein (VCP) (C-Term) antibodyVCP 适用: 人, 小鼠, 大鼠 WB, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Valosin Containing Protein (VCP) (AA 732-760) antibodyVCP 适用: 人, 小鼠, 大鼠 WB, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Immunocytochemistry: Recommended dilution: 10 μg/mL, membrane permeabilization (acetone) is essential. The antibody Hs-14 is designed for quantitative immunofluorescence analysis of pathological sperms (excellent tool for laboratories of assisted reproduction when optimal method of fertilization is sought).
Flow cytometry: Intraacrosomal staining, recommended dilution: 3-12 μg/mL. - 限制
- 仅限研究用
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- 浓度
- 1 mg/mL
- 缓冲液
- Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Do not freeze.
- 储存条件
- 4 °C
- 储存方法
- Store at 2-8°C. Do not freeze.
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: "Monoclonal antibodies against sperm intra-acrosomal antigens as markers for male infertility diagnostics and estimation of spermatogenesis." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 53, Issue 1, pp. 42-9, (2005) (PubMed).
: "[Use of human sperm protein monoclonal antibodies in the diagnosis of sperm pathology and selection of a suitable assisted reproduction method for fertilization]." in: Ceská gynekologie / Ceská lékarská spolecnost J. Ev. Purkyne, Vol. 65, Issue 1, pp. 28-32, (2000) (PubMed).
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: "Monoclonal antibodies against sperm intra-acrosomal antigens as markers for male infertility diagnostics and estimation of spermatogenesis." in: American journal of reproductive immunology (New York, N.Y. : 1989), Vol. 53, Issue 1, pp. 42-9, (2005) (PubMed).
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- 抗原
- VCP (Valosin Containing Protein (VCP))
- 别名
- VCP (VCP 产品)
- 别名
- ALS14 antibody, IBMPFD antibody, TERA antibody, p97 antibody, CDC48 antibody, wu:fd16d05 antibody, wu:fj63d11 antibody, p93 antibody, 3110001E05 antibody, p97/VCP antibody, 22.26 antibody, 7.12 antibody, Apple antibody, BcDNA.GM02885 antibody, BcDNA:GM02885 antibody, CG2331 antibody, DmTER94 antibody, Dmel\CG2331 antibody, I antibody, Ter94 antibody, Tera antibody, VCP antibody, VCP ATPase antibody, VCP/p97 antibody, Vcp antibody, anon-WO2004063362.65 antibody, anon-WO2004063362.67 antibody, cdc48 antibody, dTERA antibody, dVCP antibody, dmTERA antibody, dvcp antibody, l(2)03775 antibody, l(2)46CFf antibody, l(2)46CFs antibody, l(2)46Ch antibody, pTER94 antibody, ter94 antibody, vcp antibody, FANCG antibody, valosin containing protein antibody, valosin containing protein S homeolog antibody, valosin-containing protein antibody, CG2331 gene product from transcript CG2331-RA antibody, zgc:136908 antibody, transitional endoplasmic reticulum ATPase antibody, VCP antibody, vcp.S antibody, vcp antibody, Vcp antibody, Bm1_56585 antibody, LOC100136246 antibody, TER94 antibody, zgc:136908 antibody, LOC101109680 antibody
- 背景
- Valosin containing protein,VCP (valosin-containing protein), also known as p97, TERA, ALS14, IBMPFD, HEL-220, IBMPFD1, or HEL-S-70, is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is a structural protein that associates with clathrin and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. In sperm this intra-acrosomal protein can be used as a marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.,TERA, CDC48
- 基因ID
- 7415
- UniProt
- P55072
- 途径
- ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Ubiquitin Proteasome Pathway
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