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DYKDDDDK Tag 抗体

WB, IP, IF 宿主: 小鼠 Monoclonal unconjugated
产品编号 ABIN3020559
发货至: 中国
  • 抗原 See all DYKDDDDK Tag products
    DYKDDDDK Tag
    适用
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    宿主
    • 58
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    小鼠
    克隆类型
    • 65
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    单克隆
    标记
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    This DYKDDDDK Tag antibody is un-conjugated
    应用范围
    • 80
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    Western Blotting (WB), Immunoprecipitation (IP), Immunofluorescence (IF)
    序列
    DYKDDDDK
    纯化方法
    Affinity purification
    免疫原
    Fusion protein of human DYKDDDDK-Tag
    亚型
    IgG
  • 应用备注
    WB: 1:5000, IF: 1:2000, IP: 1:100
    限制
    仅限研究用
  • 缓冲液
    PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid freeze / thaw cycles
    储存条件
    -20 °C / -80 °C
  • Liang, Li, Jin, Xu, Pi, Liu, Zhu, Zhang, Luan, Gong, Zhang: "Pharmacological effect of human melanocortin-2 receptor accessory protein 2 variants on hypothalamic melanocortin receptors." in: Endocrine, Vol. 61, Issue 1, pp. 94-104, (2019) (PubMed).

    Liu, Xu, Zhu, Zhang, Chen, Zhou, Chen, Lin: "The calcium-dependent kinase OsCPK24 functions in cold stress responses in rice." in: Journal of integrative plant biology, Vol. 60, Issue 2, pp. 173-188, (2018) (PubMed).

    Wang, Yao, Wang, Ma, Tong, Wang, Bao, Jiang, Yang, Zhang, Xu, Wang, Zhang, Wang: "Overexpression of VpEIFP1, a novel F-box/Kelch-repeat protein from wild Chinese Vitis pseudoreticulata, confers higher tolerance to powdery mildew by inducing thioredoxin z proteolysis." in: Plant science : an international journal of experimental plant biology, Vol. 263, pp. 142-155, (2018) (PubMed).

    He, Wang, Higgins, Yu, Zong, Lu, Zhang, Liang: "MEIOTIC F-BOX Is Essential for Male Meiotic DNA Double-Strand Break Repair in Rice." in: The Plant cell, Vol. 28, Issue 8, pp. 1879-93, (2017) (PubMed).

    Jing, Zhou, Fang, Ding, Wang, Ke, Chen, Xiao: "DExD/H-Box Helicase 36 Signaling via Myeloid Differentiation Primary Response Gene 88 Contributes to NF-κB Activation to Type 2 Porcine Reproductive and Respiratory Syndrome Virus Infection." in: Frontiers in immunology, Vol. 8, pp. 1365, (2017) (PubMed).

    Liu, Xin, Wang, Wang, Wang, Zhang, Yang, Zhang, Zhang, Wang, Xu, Duan, Xia: "Estrogen receptors in granulosa cells govern meiotic resumption of pre-ovulatory oocytes in mammals." in: Cell death & disease, Vol. 8, Issue 3, pp. e2662, (2017) (PubMed).

    Ye, Chen, Li, Zhao, He, Zohaib, Song, Deng, Zhang, Chen, Cao: "Japanese Encephalitis Virus NS5 Inhibits Type I Interferon (IFN) Production by Blocking the Nuclear Translocation of IFN Regulatory Factor 3 and NF-κB." in: Journal of virology, Vol. 91, Issue 8, (2017) (PubMed).

    Jing, Fang, Ding, Wang, Hao, Gao, Ke, Chen, Xiao: "Porcine Reproductive and Respiratory Syndrome Virus nsp1α Inhibits NF-κB Activation by Targeting the Linear Ubiquitin Chain Assembly Complex." in: Journal of virology, Vol. 91, Issue 3, (2017) (PubMed).

    Chen, Ye, Ashraf, Li, Wei, Wan, Zohaib, Song, Chen, Cao: "MicroRNA-33a-5p Modulates Japanese Encephalitis Virus Replication by Targeting Eukaryotic Translation Elongation Factor 1A1." in: Journal of virology, Vol. 90, Issue 7, pp. 3722-34, (2016) (PubMed).

    Fan, Zhang, Qian, Qian, Wu, Chen, Li: "Swine TRIM21 restricts FMDV infection via an intracellular neutralization mechanism." in: Antiviral research, Vol. 127, pp. 32-40, (2016) (PubMed).

    Li, Liu, Yang, Fu, Zhao, Shen, Miao, Rayner, Chavanas, Zhu, Britt, Tang, McVoy, Luo: "Human Cytomegalovirus Infection Dysregulates the Localization and Stability of NICD1 and Jag1 in Neural Progenitor Cells." in: Journal of virology, Vol. 89, Issue 13, pp. 6792-804, (2015) (PubMed).

  • 抗原
    DYKDDDDK Tag
    别名
    FLAG tag (DYKDDDDK Tag 产品)
    物质类
    Tag
    背景
    Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(DYKDDDDK) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DYKDDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DYKDDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DYKDDDDK peptide is more likely to be accessible to antibodies. A DYKDDDDK-Tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a mouse monoclonal antibody raised against3xDYKDDDDK DYKDDDDK sequence and recognizes the (3x)DYKDDDDK peptide and detects DYKDDDDK-Tagged proteins.       
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