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Myc Tag 抗体 (AA 423-435) (Alkaline Phosphatase (AP))

WB, ELISA, IHC (p), IHC (fro), IHC 宿主: 小鼠 Monoclonal 9E10 Alkaline Phosphatase (AP)
产品编号 ABIN290539
发货至: 中国
  • 抗原 See all Myc Tag products
    Myc Tag
    抗原表位
    • 34
    • 22
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    AA 423-435
    适用
    请咨询
    宿主
    • 84
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    小鼠
    克隆类型
    • 95
    • 60
    • 10
    单克隆
    标记
    • 83
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    This Myc Tag antibody is conjugated to Alkaline Phosphatase (AP)
    应用范围
    • 132
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    Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (IHC)
    特异性
    Detects the 62kD c-myc gene product, which is involved in the regulation of the cell cycle and cell growth. p62c-myc is primarily located to the cell nucleus, but has also been shown to localized to the cytoplasm in several cell lines. Overexpression of c-myc has been reported in a wide variety of human cancers. May also be used to detect the commonly used c-myc tag.
    预测反应
    Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%) Orangutan, Monkey, Marmoset, Tamarin (92%) Rat, Sheep, Hamster, Panda, Dog, Horse (85%).
    纯化方法
    Affinity purified
    免疫原
    Synthetic peptide sequence AEEQKLISEEDLL corresponding to the C-terminal region of human c-myc. Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla (100%), Orangutan, Monkey, Marmoset, Tamarin (92%), Rat, Sheep, Hamster, Panda, Dog, Horse (85%).

    Type of Immunogen: Synthetic peptide
    克隆位点
    9E10
    亚型
    IgG1
  • 应用备注
    Approved: ELISA (1:500 - 1:5000), IHC, IHC-Fr, IHC-P, WB (1:100 - 1:500)

    Usage: Immunohistochemistry: This product does not require protein digestion pre-treatment of paraffin sections prior to staining This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections. Western Blot: LS-C57629 detects a band of approximately 62kD in Colo 320HSR cell lysates. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
    说明

    Target Species of Antibody: Human

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    PBS,  pH 7.4, 0.09 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    avoid freezing
    储存条件
    4 °C
    储存方法
    Store at 4°C. Do not freeze.
  • 抗原
    Myc Tag
    Abstract
    Myc Tag 产品
    物质类
    Tag
    UniProt
    P01106
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