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Calretinin 抗体

CALB2 适用: 人 WB, IHC (p), IF, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN2855596
发货至: 中国
  • 抗原 See all Calretinin (CALB2) 抗体
    Calretinin (CALB2) (Calbindin 2 (CALB2))
    适用
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    宿主
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    克隆类型
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    多克隆
    标记
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    This Calretinin antibody is un-conjugated
    应用范围
    • 70
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    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
    交叉反应
    人, 小鼠, 大鼠
    产品特性
    Rabbit Polyclonal antibody to Calretinin (calbindin 2)
    Calretinin antibody
    纯化方法
    Purified by antigen-affinity chromatography.
    免疫原
    Recombinant protein encompassing a sequence within the center region of human Calretinin. The exact sequence is proprietary.
    亚型
    IgG
  • 应用备注
    WB: 1:500-1:10000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
    说明

    Positive Control: A431 , Mouse brain , human CALB2-transfected 293T cells , rat brain

    Validation: Overexpression

    限制
    仅限研究用
  • 生效 #103867 (Immunohistochemistry)
    '独立验证'标志
    by
    Prof. Merighi, Laboratory of Neurobiology, Department of Veterinary Sciences, University of Turin
    No.
    #103867
    日期
    2019.05.29
    抗原
    Calretinin
    Lot Number
    39876
    Method validated
    Immunohistochemistry
    Positive Control

    Adult (24 months) mouse and dog brain

    Negative Control

    We incubated slices overnight with the blocking solution only and then processed them with the secondary antibody.

    Notes

    ABIN2855596 works in IHC-P with microwave antigen retrieval pretreatment at an optimal concentration of 1:200.

    '独立验证'标志
    Validation Images
    Full Methods
    Primary Antibody
    ABIN2855596
    Secondary Antibody
    goat anti-rabbit AF488-conjugated antibody (Invitrogen by Thermo Fisher Scientific, A11034, lot 1971418)
    Full Protocol
    • Obtain dog samples after autopsy and immersion-fix in buffered formaldehyde for 16h.
    • Perfuse mice with paraformaldehyde 4% in 0.1M phosphate buffer pH 7.4 and post-fix in the same fixative for an additional 2h at RT.
    • Wash, dehydrate, and embed samples in paraffin wax.
    • Cut with a microtome into 6µm sections and mount on glass slides.
    • After paraffin removal, process sections by microwave antigen retrieval for 10min (95-100°C) in 10mM sodium citrate buffer (pH 6.0).
    • Let sections cool for 10min.
    • Wash sections under running water for 5-10min.
    • Wash sections 2x 5min with distilled water.
    • Wash sections 5min with 0.01M PBS.
    • Incubate sections in PBS containing 1% albumin from chicken egg white (Sigma, A5378) for 1h at RT to block non-specific binding sites.
    • Incubate sections with primary rabbit anti-Calretinin antibody (antibodies-online, ABIN2855596, lot 39876) diluted 1:200 in PBS-BSA-PLL at 4°C.
    • Wash sections 3x 5min with 0.01M PBS.
    • Incubate sections with secondary goat anti-rabbit AF488-conjugated antibody (Invitrogen by Thermo Fisher Scientific, A11034, lot 1971418) diluted 1:500 in 0.1M PBS for 1h at RT.
    • Wash sections 3x 5min with 0.01M PBS.
    • Mount specimens in Fluoroshield (Sigma, F6182, lot MKCB0153V).
    • Acquire images with a fluorescence microscope and appropriate filter settings for AF488, e.g. Leica DM 6000B fluorescence microscope equipped with the manufacturer’s FITC filter set and digital camera at magnifications of 20x, 40x, and 63x. Parameters for image acquisition (Exposure 2.2s, Gain 2.3x, Saturation 1.5, Gamma 1.10) unchanged for all images.
    Experimental Notes

    For the incubation with the primary antibody ABIN2669973 1:100, 1:500, and 1:1000 dilutions were also tested.

  • 状态
    Liquid
    浓度
    0.75 mg/mL
    缓冲液
    0.1M Tris-Glycine ( pH 7), 10 % Glycerol, 0.01 % Thimerosal
    储存液
    Thimerosal (Merthiolate)
    注意事项
    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Bradshaw, Trossbach, Köber, Walter, Prikulis, Weggen, Korth: "Disrupted in Schizophrenia 1 regulates the processing of reelin in the perinatal cortex." in: Schizophrenia research, Vol. 215, pp. 506-513, (2021) (PubMed).

  • 抗原
    Calretinin (CALB2) (Calbindin 2 (CALB2))
    别名
    calbindin 2 (CALB2 产品)
    别名
    CAB29 antibody, CAL2 antibody, CR antibody, calb2l antibody, wu:fq18e08 antibody, zgc:73115 antibody, calb2 antibody, wu:fq17g09 antibody, zgc:73099 antibody, calbindin 2 antibody, calbindin 2a antibody, calbindin 2b antibody, CALB2 antibody, Calb2 antibody, calb2a antibody, calb2b antibody
    背景
    This gene encodes an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. Three alternatively spliced transcript variants that encode different proteins have been described.

    分子量
    32 kDa
    基因ID
    794
    UniProt
    P22676
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