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PAX8 抗体

Name: PAX8 抗体
Brand: antibodies-online
SKU: ABIN2855097
Availability: OutOfStock

KO Validated PAX8 适用: 人 WB, IHC, IF, IHC (p), IP, ICC, ChIP 宿主: 兔 Polyclonal unconjugated

WB Image Sample(30 ug whole cell lysate) A:293T 10% SDS PAGE antibody diluted at 1:1000

IP Image PAX8 antibody immunoprecipitates PAX8 protein in IP experiments.

ChIP

ICC/IF Image Immunofluorescence analysis of paraformaldehyde-fixed A549, using PAX8, antibody at 1:200 dilution.

IHC-P Image PAX8 antibody detects PAX8 protein at cytosol and nucleus on rat thyroid gland by immunohistochemical analysis.

IHC

PAX8 knockdown decreased FGF18 production and cellular motility.

The PAX8-FGF18 signaling axis promotes tumor cell motility.

Class I ,but not class II, HDAC inhibitors reduced PAX8 expression.

IF (cc)

8 images

(2 references)

产品编号 ABIN2855097

发货至: 中国

Datasheet as PDF

抗原 See all PAX8 抗体

PAX8 (Paired Box 8 (PAX8))
适用
111

48

45

20

20

6

5

5

4

2

2

1
人
宿主
72

38

1
兔
Compatible Secondaries
克隆类型
56

55
多克隆
标记
62

8

6

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1
This PAX8 antibody is un-conjugated
应用范围
87

36

31

30

25

16

15

12

11

6

5

4

2

2

1

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

交叉反应

犬, 人, 小鼠, 大鼠

产品特性

Rabbit Polyclonal antibody to PAX8 (paired box 8)
PAX8 antibody

纯化方法

Purified by antigen-affinity chromatography.

质量等级

KO Validated

免疫原

Recombinant protein encompassing a sequence within the center region of human PAX8. The exact sequence is proprietary.

亚型

IgG
anti-Paired Box 8 (PAX8) (AA 60-261) antibody
PAX8 适用: 人 WB, IHC, FACS, IF, StM 宿主: 小鼠 Monoclonal PAX8-1492 unconjugated

anti-Paired Box 8 (PAX8) (AA 60-261) antibody
PAX8 适用: 人 WB, ELISA, IHC, FACS 宿主: 小鼠 Monoclonal 1A1D3 unconjugated

anti-Paired Box 8 (PAX8) (AA 60-261) antibody
PAX8 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal PAX8-1491 unconjugated

anti-Paired Box 8 (PAX8) (AA 1-450) antibody
PAX8 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3H7 unconjugated

anti-Paired Box 8 (PAX8) (AA 300-377) antibody
PAX8 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2D6 unconjugated

anti-Paired Box 8 (PAX8) (AA 300-377) antibody
PAX8 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 3G11 unconjugated

anti-Paired Box 8 (PAX8) (AA 141-250) antibody
PAX8 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2F1 unconjugated

anti-Paired Box 8 (PAX8) (N-Term) antibody
Verified PAX8 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated

anti-Paired Box 8 (PAX8) (AA 300-450) antibody
PAX8 适用: 人 WB, ELISA, FACS 宿主: 小鼠 Monoclonal 3E6B3 unconjugated

anti-Paired Box 8 (PAX8) (AA 285-313) antibody
PAX8 适用: 人 WB, ELISA, IHC, FACS 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:5000-1:20000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:500-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: 293T nuclear extract , DDDDK-tagged PAX8-transfected 293T
Validation: KO/KD, Orthogonal, Overexpression

限制

仅限研究用
状态

Liquid

浓度

0.87 mg/mL

缓冲液

1XPBS ( pH 7), 20 % Glycerol, 0.025 % ProClin 300

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Shi, Yin, Cai, Yan, Jia, Ma, Zhang, Zhang, Gu, Zhang, Di, Zhuang: "PAX8 regulon in human ovarian cancer links lineage dependency with epigenetic vulnerability to HDAC inhibitors." in: eLife, Vol. 8, (2020) (PubMed).

Sun, Lu, Wu, Ouyang, Xing, Hou, Shi, Wu: "PTEN-knockdown disrupts the morphology, growth pattern and function of Nthy-Ori 3-1 cells by downregulating PAX8 expression." in: Oncology letters, Vol. 18, Issue 6, pp. 6732-6740, (2019) (PubMed).
抗原

PAX8 (Paired Box 8 (PAX8))

别名

paired box 8 (PAX8 产品)

别名

PAX8 antibody, XPax-8 antibody, XPax8 antibody, pax-8 antibody, PAX-8 antibody, Pax-8 antibody, paired box 8 antibody, paired box 8 L homeolog antibody, PAX8 antibody, pax8 antibody, Pax8 antibody, pax8.L antibody

背景

This gene encodes a member of the paired box (PAX) family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternateively spliced transcript variants encoding different isoforms have been described.

Cellular Localization: Nucleus

分子量

48 kDa

基因ID

7849

UniProt

Q06710

途径

Thyroid Hormone Synthesis, Regulation of Hormone Metabolic Process, Stem Cell Maintenance, Tube Formation