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MTOR 抗体 (C-Term)

Name: MTOR 抗体 (C-Term)
Brand: antibodies-online
SKU: ABIN2855088
Availability: OutOfStock

mTOR 适用: 人, 小鼠, 大鼠 WB, IF, IHC (p), IP, ICC, ChIP 宿主: 兔 Polyclonal unconjugated

WB Image mTOR antibody detects mTOR protein by western blot analysis.

ChIP

IHC-P Image mTOR antibody detects FRAP1 protein at cytosol on human hepatoma by immunohistochemical analysis.

IHC

IP Image Immunoprecipitation of mTOR protein from 293T whole cell extracts using 5 μg of mTOR antibody [C3], C-term, Western blot analysis was performed using mTOR antibody [C3], C-term, EasyBlot anti-Rabbit IgG was used as a secondary reagent.

IHC-P Image mTOR antibody [C3], C-term detects mTOR protein at mitochondria on mouse stomach by immunohistochemical analysis.

IHC

ICC/IF Image mTOR antibody detects mTOR protein at cytoplasm and nucleus by immunofluorescent analysis.

IHC-P Image mTOR antibody [C3], C-term detects mTOR protein at cytoplasm in mouse testis by immunohistochemical analysis.

IHC

IP Image mTOR antibody immunoprecipitates mTOR protein in IP experiments.

MIF facilitates autophagy induction in endothelial cells.

BMP4 inhibits triglyceride accumulation through regulating the genes involved in lipid metabolism and members of mTORC1 signaling pathway in hepatocytes.

BMP4 down-regulates the mTORC1 signaling pathway in mouse liver.

IHC

Exogenous BMP4 attenuates the development and progression of NAFLD by suppressing the mTORC1 signaling pathway.

IHC

15 images

(5 references)

产品编号 ABIN2855088

发货至: 中国

Datasheet as PDF

抗原 See all MTOR (mTOR) 抗体

MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))
抗原表位
34

28

22

15

11

10

9

7

5

5

4

4

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term
适用
209

139

120

23

17

12

7

6

6

5

5

3

2

1

1

1
人, 小鼠, 大鼠
宿主
190

15

2

2
兔
Compatible Secondaries
克隆类型
171

37
多克隆
标记
106

11

10

7

7

7

7

7

7

7

6

4

4

4

4

4

3

1

1

1

1
This MTOR antibody is un-conjugated
应用范围
144

64

63

52

52

43

35

34

16

16

12

7

3

2

2

2
Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

产品特性

Rabbit Polyclonal antibody to mTOR (mechanistic target of rapamycin (serine/threonine kinase))
mTOR antibody [C3], C-term

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Recombinant protein encompassing a sequence within the C-terminus region of human mTOR. The exact sequence is proprietary.

亚型

IgG

Top Product

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anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2436-2492) antibody
mTOR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2226-2488) antibody
mTOR 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2400-2500) antibody
mTOR 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) antibody
mTOR 适用: 人, 小鼠, 大鼠, 鱼 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2311-2529) antibody
mTOR 适用: 人 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 8H5A5 unconjugated

anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2459-2488) antibody
mTOR 适用: 人 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB13347 unconjugated

anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2459-2488) antibody
mTOR 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated

anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (pSer2448) antibody
mTOR 适用: 人, 小鼠, 大鼠, Cow, 鱼 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (pSer2481) antibody
mTOR 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR) (AA 2226-2488) antibody
mTOR 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal D4 unconjugated

应用备注

Suggested dilution Reference ChIP assay Assay-dependent dilution ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceChIP assayAssay-dependent dilution ICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

说明

Positive Control: 293T , A431 , HeLa , HepG2 , mouse brain , *Flag-human mTOR transfected 293T

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

1XPBS, 20 % Glycerol ( pH 7). 0.025 % ProClin 300 was added as a preservative.

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

-20 °C

储存方法

Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Wang, Cao, Shu, Zhu, Peng, Ran, Wu, Luo, Zuo, Luo, Zhou, Shi, Weng, Huang, He, Fan: "Long non-coding RNA (lncRNA) H19 induces hepatic steatosis through activating MLXIPL and mTORC1 networks in hepatocytes." in: Journal of cellular and molecular medicine, Vol. 24, Issue 2, pp. 1399-1412, (2020) (PubMed).

Cheung, Hsu, Lin, Chen, Wang, Huang, Juan: "ZNF322A-mediated protein phosphorylation induces autophagosome formation through modulation of IRS1-AKT glucose uptake and HSP-elicited UPR in lung cancer." in: Journal of biomedical science, Vol. 27, Issue 1, pp. 75, (2020) (PubMed).

Peng, Chen, Wang, Zhu, Wu, Luo, Zuo, Luo, Zhou, Shi, Weng, Huang, He, Fan: "Bone morphogenetic protein 4 (BMP4) alleviates hepatic steatosis by increasing hepatic lipid turnover and inhibiting the mTORC1 signaling axis in hepatocytes." in: Aging, Vol. 11, Issue 23, pp. 11520-11540, (2019) (PubMed).

Li, Yu, Jiao, Wang, Zhang, Sun: "Vandetanib (ZD6474) induces antiangiogenesis through mTOR-HIF-1 alpha-VEGF signaling axis in breast cancer cells." in: OncoTargets and therapy, Vol. 11, pp. 8543-8553, (2018) (PubMed).

Xu, Wu, Zheng, Yu, Yang, Yao, Zhou, Ching, Lau: "Proteome profiling of cadmium-induced apoptosis by antibody array analyses in human bronchial epithelial cells." in: Oncotarget, Vol. 7, Issue 5, pp. 6146-58, (2017) (PubMed).
抗原

MTOR (mTOR) (Mechanistic Target of Rapamycin (serine/threonine Kinase) (mTOR))

别名

mTOR (mTOR 产品)

别名

FRAP1 antibody, FRAP antibody, FRAP2 antibody, RAFT1 antibody, RAPT1 antibody, 2610315D21Rik antibody, AI327068 antibody, Frap1 antibody, flat antibody, frap1 antibody, tor antibody, wu:fc22h08 antibody, mechanistic target of rapamycin kinase antibody, mechanistic target of rapamycin antibody, mechanistic target of rapamycin (serine/threonine kinase) antibody, MTOR antibody, Mtor antibody, mtor antibody

背景

The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The ANGPTL7 gene is located in an intron of this gene.

Cellular Localization: Endoplasmic reticulum membrane, Peripheral membrane protein, Cytoplasmic side , Golgi apparatus membrane, Peripheral membrane protein, Cytoplasmic side , Mitochondrion outer membrane, Peripheral membrane protein, Cytoplasmic side

分子量

289 kDa

基因ID

2475

途径

PI3K-Akt Signaling, RTK signaling, AMPK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Autophagy, CXCR4-mediated Signaling Events, BCR Signaling, Warburg Effect