HMGB1 抗体
(4 references)-
- 抗原 See all HMGB1 抗体
- HMGB1 (High Mobility Group Box 1 (HMGB1))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This HMGB1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Whole Mount) (IHC (wm)), Immunohistochemistry (Free Floating) (IHC (ff))
- 交叉反应
- 人, 小鼠, Pig, 大鼠, 斑马鱼
- 产品特性
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Rabbit Polyclonal antibody to HMGB1 (high-mobility group box 1)
HMGB1 antibody - 纯化方法
- Purified by antigen-affinity chromatography.
- 质量等级
- KO Validated
- 免疫原
- Recombinant protein encompassing a sequence within the center region of human HMGB1. The exact sequence is proprietary.
- 亚型
- IgG
- Top Product
- Discover our top product HMGB1 Primary Antibody
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anti-High Mobility Group Box 1 (HMGB1) (AA 75-170) antibody
HMGB1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 1-90) antibodyHMGB1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2F6 unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 100-200) antibodyHMGB1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 9-163) antibodyHMGB1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibodyHMGB1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibodyHMGB1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibodyHMGB1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1D5 unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibodyHMGB1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1E6-E10 unconjugated
anti-High Mobility Group Box 1 (HMGB1) antibodyKO Validated HMGB1 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated
anti-High Mobility Group Box 1 (HMGB1) (AA 124-154), (C-Term) antibodyHMGB1 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.
- 说明
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Positive Control: NIH-3T3 , JC , BCL-1 , C2C12 , Raw264.7 , PC-12 , HMGB1-transfected 293T
Validation: KO/KD, Orthogonal, Overexpression
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.46 mg/mL
- 缓冲液
- 1XPBS pH 7, 1 % BSA, 20 % Glycerol, 0.025 % ProClin 300
- 储存液
- ProClin
- 注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
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: "N-(2'-Hydroxyphenyl)-2-Propylpentanamide (HO-AAVPA) Inhibits HDAC1 and Increases the Translocation of HMGB1 Levels in Human Cervical Cancer Cells." in: International journal of molecular sciences, Vol. 21, Issue 16, (2020) (PubMed).
: "N-(2'-Hydroxyphenyl)-2-propylpentanamide (OH-VPA), a histone deacetylase inhibitor, induces the release of nuclear HMGB1 and modifies ROS levels in HeLa cells." in: Oncotarget, Vol. 9, Issue 70, pp. 33368-33381, (2018) (PubMed).
: "Dual therapeutic functions of F-5 fragment in burn wounds: preventing wound progression and promoting wound healing in pigs." in: Molecular therapy. Methods & clinical development, Vol. 3, pp. 16041, (2016) (PubMed).
: "Receptor for Advanced Glycation End Products and its Inflammatory Ligands are Upregulated in Amyotrophic Lateral Sclerosis." in: Frontiers in cellular neuroscience, Vol. 9, pp. 485, (2016) (PubMed).
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: "N-(2'-Hydroxyphenyl)-2-Propylpentanamide (HO-AAVPA) Inhibits HDAC1 and Increases the Translocation of HMGB1 Levels in Human Cervical Cancer Cells." in: International journal of molecular sciences, Vol. 21, Issue 16, (2020) (PubMed).
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- 抗原
- HMGB1 (High Mobility Group Box 1 (HMGB1))
- 别名
- high mobility group box 1 (HMGB1 产品)
- 背景
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DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells.
Cellular Localization: Nucleus - 分子量
- 25 kDa
- 基因ID
- 3146
- UniProt
- P09429
- 途径
- p53 Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome
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