Myc 标记 抗体 (Biotin)

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抗原
  • MRTL
  • MYCC
  • bHLHe39
  • c-Myc
  • ARC
  • B430311C09Rik
  • MYC
  • NOP
  • Nop30
  • MYC proto-oncogene, bHLH transcription factor
  • nucleolar protein 3 (apoptosis repressor with CARD domain)
  • MYC
  • Nol3
适用
标记
211
58
14
12
6
5
3
1
1
宿主
小鼠
166
64
20
17
6
5
3
1
克隆类型 (克隆位点)
单克隆 ()
标记
Biotin
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23
15
9
5
5
4
4
4
4
3
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
应用范围
Immunofluorescence (IF), Western Blotting (WB)
239
118
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50
39
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36
23
7
6
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5
4
4
4
3
3
3
2
1
1
1
1
1
1
1
选项
Supplier
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Supplier Product No.
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免疫原 GST-6myc-Tag fusion protein
序列 EQKLISEEDL
克隆位点 PL14
亚型 IgG1
特异性 This antibody recognizes Myc-Tag peptide sequence (EQKLISEEDL).
纯化方法 Protein-A Sepharose
别名 C-Myc Epitope Tag
物质类 Tag
背景 Epitope tagging has widely been accepted technique that fuse an epitope peptide to a certain protein as a marker for gene expression. With this technique, the gene expression can be easily monitored on western blotting, immunoprecipitation and immunofluorescence utilizing with an antibody that recognizes such an epitope. Amino acid sequences that are widely used for the epitope tagging are as follow, YPYDVPDYA (HA-Tag), EQKLISEEDL (Myc-Tag) and YTDIEMNRLGK (VAV-G- Tag), which corresponding to the partial peptide of Influenza hemagglutinin protein, Human c-myc gene product and Vesicular stomatitis virus glycoprotein respectively.Synonyms: c-myc tag, myc tag, myc-tag
基因ID 4609
NCBI登录号 NP_002458
UniProt P01106
应用备注 Optimal working dilution should be determined by the investigator.
限制 仅限研究用
缓冲液 NaPB ( pH 8.0), 0.3 M NaCl and 0.09 % NaN3
储存液 Sodium azide
注意事项 This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件 4 °C
储存方法 Store at 2 - 8 °C.
背景 Heald, McLoughlin, McKeon: "Human wee1 maintains mitotic timing by protecting the nucleus from cytoplasmically activated Cdc2 kinase." in: Cell, Vol. 74, Issue 3, pp. 463-74, 1993 (PubMed).

Field, Nikawa, Broek, MacDonald, Rodgers, Wilson, Lerner, Wigler: "Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method." in: Molecular and cellular biology, Vol. 8, Issue 5, pp. 2159-65, 1988 (PubMed).

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