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HMGB1 抗体 (N-Term)

Name: HMGB1 抗体 (N-Term)
SKU: ABIN2787911
Availability: OutOfStock
Rating: 5 (1 reviews)

HMGB1 适用: 人, 小鼠, 大鼠, Cow, 犬, 兔, 马, 豚鼠, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated

Western Blotting validation image for anti-High Mobility Group Box 1 (HMGB1) (N-Term) antibody (ABIN2787911)

WB Suggested Anti-HMGB1 Antibody Titration: 0.

3 images

(1 validation)

产品编号 ABIN2787911

发货至: 中国

Datasheet as PDF

抗原 See all HMGB1 抗体

HMGB1 (High Mobility Group Box 1 (HMGB1))
抗原表位
21

16

16

10

10

8

6

6

6

6

5

4

3

3

3

3

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1
N-Term
适用
155

87

86

13

12

11

10

8

7

6

6

5

5

2

1

1
人, 小鼠, 大鼠, Cow, 犬, 兔, 马, 豚鼠, 斑马鱼
宿主
135

30

2

2

1

1
兔
Compatible Secondaries
克隆类型
125

46
多克隆
标记
88

17

17

7

7

6

3

3

3

3

3

3

2

2

2

2

2

1
This HMGB1 antibody is un-conjugated
应用范围
144

64

46

46

33

30

27

26

21

10

9

5

2

2

1

1

1

1

1

1
Western Blotting (WB)

序列

QTCREEHKKK HPDASVNFSE FSKKCSERWK TMSAKEKGKF EDMAKADKAR

预测反应

Cow: 100%, Dog: 100%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Zebrafish: 93%

产品特性

This is a rabbit polyclonal antibody against HMGB1. It was validated on Western Blot.

纯化方法

Affinity Purified

免疫原

The immunogen is a synthetic peptide directed towards the N terminal region of human HMGB1

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HMGB1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-High Mobility Group Box 1 (HMGB1) (AA 1-90) antibody
HMGB1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 2F6 unconjugated

anti-High Mobility Group Box 1 (HMGB1) (AA 100-200) antibody
HMGB1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-High Mobility Group Box 1 (HMGB1) antibody
KO Validated HMGB1 适用: 人 WB, IHC, IF, IHC (p), ICC, IHC (wm), IHC (ff) 宿主: 兔 Polyclonal unconjugated

anti-High Mobility Group Box 1 (HMGB1) (AA 9-163) antibody
HMGB1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibody
HMGB1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibody
HMGB1 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibody
HMGB1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1D5 unconjugated

anti-High Mobility Group Box 1 (HMGB1) (AA 1-215) antibody
HMGB1 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1E6-E10 unconjugated

anti-High Mobility Group Box 1 (HMGB1) antibody
KO Validated HMGB1 适用: 人 WB, IHC, IF 宿主: 兔 Monoclonal unconjugated

应用备注

Optimal working dilutions should be determined experimentally by the investigator.

说明

Antigen size: 215 AA

限制

仅限研究用
生效 #103662 (Western Blotting)

by

Lewis Lab, Department of Biology, College of Arts and Sciences, Syracuse University

No.

#103662

日期

2019.12.05

抗原

HMGB1

Lot Number

QC29977-40568

Method validated

Western Blotting

Positive Control

Zebrafish embryos injected with Myc-Hmgb1a

Negative Control

Uninjected embryos

Notes

Passed. ABIN2787911 specifically recognizes Myc-Hmgb1a in embryos overexpressing this construct.

Validation Images
Full Methods
Primary Antibody

ABIN2787911

Secondary Antibody

IRDye 800CW Goat anti-Rabbit IgG Secondary Antibody (LI-COR, 925-32211, lot C80925-01)

Full Protocol

Harvest >100 zebrafish embryos from wild type parents.
Inject >50 embryos with 1ng mRNA encoding a Myc-tagged Hmgb1a construct. Leave the remainder of embryos uninjected as negative controls.
Grow embryos to 6h post-fertilization.
Enzymatically dechorionate all surviving, fertile embryos with 1mg/ml Pronase solution.
De-yolk embryos mechanically by pipetting up and down several times, spinning down cells, and washing away soluble yolk proteins.
Lyse each group of embryos mechanically with micropestle in 1µl per embryo of lysis buffer (50mM Tris-HCl pH8.0, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 1x Roche EDTA-free protease inhibitor cocktail, 1mM PMSF).
Spin down debris and transfer lysates to fresh tubes.
Mix lysates 1:1 with 2x Laemmli SDS sample buffer and incubate 5min at 95°C.
Load 20µl of resulting mix per well of 4-20% Mini-PROTEAN TGX precast protein gel (Bio-Rad, 456-1095, lot 64268988) in a Mini-PROTEAN Electrophoresis Cell and run for 40min at 200V.
Activate 0.45µm PVDF membrane (Millipore, product IPVH00010, lot K3HA8233EK) with methanol. Transfer proteins to membrane with transfer buffer (25mM Tris-HCl pH8.3, 192mM glycine, 20% v/v methanol) for 1h at 100V.
Rinse membrane with water, then incubate 2min in PBS.
Block the membrane with Odyssey Blocking Buffer (LI-COR, P/N 927-40000) 1h at RT with shaking.
Incubate with either of primary
rabbit anti-HMGB1 antibody (antibodies-online, ABIN2787911, lot QC29977-40568) diluted 1:1000 in Odyssey Blocking Buffer (LI-COR, product no, lot no) containing 0.2% Tween 20 ON at 4°C with shaking.
mouse anti-Myc antibody (ThermoFisher Scientific, MA1-980, lot NJ17123) diluted 1:1000 in Odyssey Blocking Buffer (LI-COR, product no, lot no) containing 0.2% Tween 20 ON at 4°C with shaking.
Wash membrane 5x 5min each with PBST (PBS, 0.1% Tween 20).
Incubate with secondary
IRDye 800CW Goat anti-Rabbit IgG Secondary Antibody (LI-COR, 925-32211, lot C80925-01) diluted 1:15000 in Odyssey Blocking Buffer containing 0.2% Tween 20 and 0.01% w/v SDS 1h at RT with shaking.
IRDye 800CW Goat anti-Mouse IgG Secondary Antibody (LI-COR, 925-32210, lot C70712-11) diluted 1:15000 in Odyssey Blocking Buffer containing 0.2% Tween 20 and 0.01% w/v SDS 1h at RT with shaking.
Wash membrane 5x 5min each with PBST.
Rinse membrane with PBS then hang to dry.
Once dry, scan the membrane with LI-COR Odyssey CLX Scanner.

Experimental Notes

The Hmgb1 antibody ABIN2787911 reveals a protein of the expected molecular weight of the overexpressed Myc-Hmgb1a construct in the injected lysate. A Myc-tag antibody also detects this protein at the same size in the same lysate. This band is not detected by ABIN2787911 in an uninjected control lysate.
ABIN2787911 also detects a faint band (or set of overlapping bands) at the expected size of endogenous Hmgb1a and Hmgb1b in both injected and uninjected lysates, but this band is weaker than several other non-specific bands.
Protein amounts of Hmgb1a and Hmgb1a at this stage are unknown, so detection of these endogenous proteins was not necessarily guaranteed. Both corresponding mRNAs are expressed at this stage, so we predicted we might be able to detect the encoded proteins.
Expected sizes of target antigens: are 25.1 kDa for pCS2-Myc-Hmgb1a, 23.7kDa for endogenous Hmgb1a: 23.7kDa, and 22.9kDa for endogenous Hmgb1b: 22.9kDa.
状态

Liquid

浓度

Lot specific

缓冲液

Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freeze-thaw cycles.

储存条件

-20 °C

储存方法

For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
抗原

HMGB1 (High Mobility Group Box 1 (HMGB1))

别名

HMGB1 (HMGB1 产品)

别名

HMG1 antibody, HMG3 antibody, SBP-1 antibody, DEF antibody, HMG-1 antibody, Hmg1 antibody, amphoterin antibody, p30 antibody, hmgb1 antibody, ik:tdsubc_1a5 antibody, wu:fb23c02 antibody, xx:tdsubc_1a5 antibody, zgc:56110 antibody, zgc:77104 antibody, hmg-1 antibody, hmg3 antibody, sbp-1 antibody, hmg1 antibody, HMGB1 antibody, Ac2-008 antibody, high mobility group box 1 antibody, high-mobility group box 1 antibody, high mobility group box 1a antibody, high mobility group box 1 L homeolog antibody, high mobility group protein B1 antibody, HMGB1 antibody, Hmgb1 antibody, hmgb1 antibody, hmgb1a antibody, hmgb1.L antibody, LOC100359149 antibody

背景

Extracellular HMGB1 is an activator of human tumor cell migration operating in concert with EGF. HMGB1 encodes a protein that is potentially involved in the regulation of lipogenic and cholesterogenic gene transcription.
Alias Symbols: DKFZp686A04236, HMG1, HMG3, SBP-1
Protein Interaction Partner: AGTRAP, HUWE1, UBC, ASB2, ASB1, ZNF24, MAPK1, HMGA1, ITGA4, FN1, HNF1A, SSRP1, SMARCE1, PSEN1, XPC, XPA, RAD23B, CDK2, TFAP4, TERF2IP, TERF2, HHV8GK18_gp81, SUMO2, FCP1, DNM2, HSPA8, HMGB2, PDIA3, Ccdc15, POU5F1, H3F3A, CREBBP, CDK1, TBP, LRIF1, RASSF4, N
Protein Size: 215

分子量

25 kDa

基因ID

3146

NCBI登录号

NM_002128, NP_002119

UniProt

P09429

途径

p53 Pathway, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome