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Integrin beta 2 抗体

ITGB2 适用: 人 FACS, WB, IP, Func 宿主: 小鼠 Monoclonal MEM-148 unconjugated
产品编号 ABIN2749020
发货至: 中国
  • 抗原 See all Integrin beta 2 (ITGB2) 抗体
    Integrin beta 2 (ITGB2)
    适用
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    宿主
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    小鼠
    克隆类型
    • 124
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    单克隆
    标记
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    This Integrin beta 2 antibody is un-conjugated
    应用范围
    • 121
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    Flow Cytometry (FACS), Western Blotting (WB), Immunoprecipitation (IP), Functional Studies (Func)
    特异性
    The antibody MEM-148 recognizes an extracellular epitope on CD18 which is essentially inaccessible in intact integrin molecules on resting leukocytes, but is exposed on high-affinity state of LFA-1 or on unassociated CD18. CD18 (integrin beta2 subunit, beta2 integrin) is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
    无交叉反应
    Pig
    交叉反应 (详细)
    Human
    纯化方法
    Purified by protein-A affinity chromatography.
    纯度
    > 95 % (by SDS-PAGE)
    内毒素水平
    Endotoxin level is less than 0.01 EU/µg of the protein, as determined by the LAL test.
    免疫原
    Peripheral blood mononuclear cells
    克隆位点
    MEM-148
    亚型
    IgG1
    Top Product
    Discover our top product ITGB2 Primary Antibody
  • 应用备注
    Functional application: The antibody MEM-148 induces homotypic cell aggregation and high-affinity LFA-1 conformation in resting leukocytes.
    Flow cytometry: Recommended dilution: 1-5 μg/mL. The antibody MEM-148 is an excellent marker of activated myeloid cells.
    Western blotting: Non-reducing conditions.
    限制
    仅限研究用
  • 浓度
    1 mg/mL
    缓冲液
    Phosphate buffered saline (PBS), pH 7.4
    储存液
    Azide free
    储存条件
    4 °C
    储存方法
    Store at 2-8°C. Do not freeze.
  • Aubert, Yoon, Sloan, Spear, Jerome: "The virological synapse facilitates herpes simplex virus entry into T cells." in: Journal of virology, Vol. 83, Issue 12, pp. 6171-83, (2009) (PubMed).

    Drbal, Angelisová, Cerný, Hilgert, Horejsí: "A novel anti-CD18 mAb recognizes an activation-related epitope and induces a high-affinity conformation in leukocyte integrins." in: Immunobiology, Vol. 203, Issue 4, pp. 687-98, (2001) (PubMed).

    Drbal, Angelisová, Hilgert, Cerný, Novák, Horejsí: "A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells." in: Blood, Vol. 98, Issue 5, pp. 1561-6, (2001) (PubMed).

    Drbal, Angelisová, Cerný, Pavlistová, Cebecauer, Novák, Horejsí: "Human leukocytes contain a large pool of free forms of CD18." in: Biochemical and biophysical research communications, Vol. 275, Issue 2, pp. 295-9, (2000) (PubMed).

  • 抗原
    Integrin beta 2 (ITGB2)
    别名
    CD18 (ITGB2 产品)
    别名
    CD18 antibody, LAD antibody, LCAMB antibody, LFA-1 antibody, MAC-1 antibody, MF17 antibody, MFI7 antibody, 2E6 antibody, AI528527 antibody, Cd18 antibody, Lfa1 antibody, ITGB2 antibody, itgb2 antibody, itgb2-a antibody, itgb2-b antibody, integrin subunit beta 2 antibody, integrin beta 2 antibody, integrin subunit beta 2 S homeolog antibody, ITGB2 antibody, Itgb2 antibody, itgb2.S antibody
    背景
    Integrin subunit beta 2,CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 Molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.,Integrin beta2, Complement receptor C3 subunit beta, ITGB2
    基因ID
    3689
    UniProt
    P05107
    途径
    NF-kappaB Signaling, Activation of Innate immune Response, Toll-Like Receptors Cascades, Activated T Cell Proliferation
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