CSTF1 抗体
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- 抗原 See all CSTF1 抗体
- CSTF1 (Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 1, 50kDa (CSTF1))
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CSTF1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 特异性
- This antibody detects endogenous levels of CstF-50 protein (region surrounding Arg3)
- 交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - 纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
- 纯度
- > 95 % pure by SDS-PAGE
- Top Product
- Discover our top product CSTF1 Primary Antibody
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anti-Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 1, 50kDa (CSTF1) (N-Term) antibody
CSTF1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 1, 50kDa (CSTF1) (AA 332-431) antibodyCSTF1 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1C6 unconjugated
anti-Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 1, 50kDa (CSTF1) (N-Term) antibodyCSTF1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 1, 50kDa (CSTF1) (N-Term) antibodyCSTF1 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 1, 50kDa (CSTF1) (N-Term) antibodyCSTF1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 1, 50kDa (CSTF1) (AA 185-408) antibodyCSTF1 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 1, 50kDa (CSTF1) (AA 1-50) antibodyCSTF1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 1, 50kDa (CSTF1) (AA 1-220) antibodyCSTF1 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 1, 50kDa (CSTF1) (N-Term) antibodyCSTF1 适用: 人, 小鼠, 大鼠, Cow, Pig WB, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1,0 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- DO NOT FREEZE!
- 储存条件
- 4 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C.
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- 抗原
- CSTF1 (Cleavage Stimulation Factor, 3' Pre-RNA, Subunit 1, 50kDa (CSTF1))
- 别名
- CSTF1 (CSTF1 产品)
- 别名
- fc22d10 antibody, zgc:66207 antibody, zgc:77560 antibody, wu:fc22d10 antibody, wu:fd16h09 antibody, MGC53063 antibody, MGC69421 antibody, MGC83790 antibody, 1700057K18Rik antibody, AI788832 antibody, CstF-50 antibody, CstFp50 antibody, cleavage stimulation factor, 3' pre-RNA, subunit 1 antibody, cleavage stimulation factor subunit 1 antibody, cleavage stimulation factor, 3' pre-RNA, subunit 1, 50kDa antibody, cstf1 antibody, cstf1.S antibody, CSTF1 antibody, cstf1.L antibody, Cstf1 antibody
- 背景
- Polyadenylation of mRNA precursors is a two-step reaction that requires multiple protein factors. The first step, endonucleolytic cleavage of polyadenylation substrates, requires CstF (cleavage stimulation factor), a heterotrimer that is composed of three distinct subunits of 77, 64 and 50 kDa. Heterotrimeric CstF recognizes GU and U-rich sequences located downstream of the polyadenylation site on RNA. The 50 kDa CstF subunit shares extensive homology with mammalian G protein beta-subunits and has a transducin repeat domain, which is a 44 amino acid-long sequence that is repeated seven times. CstF-50 interacts with the nuclear protein BARD1 (BRCA1-associated RING domain protein) and inhibits polyadenylation in vitro. CstF-50 may also be responsible for the interaction of the heterotrimeric CstF complex with other polyadenylation and 3'-end cleavage factors to form a stable complex on the pre-mRNA.Synonyms: CF-1 50 kDa subunit, CSTF 50 kDa subunit, Cleavage stimulation factor 50 kDa subunit, CstF-50, CstFp50
- 分子量
- approx. 48 kDa
- 基因ID
- 1477
- NCBI登录号
- NP_001028693
- UniProt
- Q05048
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