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SUPT3H/SPT3 抗体

SUPT3H 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN272144
发货至: 中国
  • 抗原 See all SUPT3H/SPT3 (SUPT3H) 抗体
    SUPT3H/SPT3 (SUPT3H) (Suppressor of Ty 3 Homolog (SUPT3H))
    适用
    • 25
    • 3
    • 3
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    • 2
    • 2
    • 2
    • 2
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    • 2
    • 1
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    • 1
    宿主
    • 24
    • 2
    克隆类型
    • 24
    • 2
    多克隆
    标记
    • 14
    • 2
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    • 1
    • 1
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    This SUPT3H/SPT3 antibody is un-conjugated
    应用范围
    • 17
    • 13
    • 9
    • 3
    • 3
    • 1
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    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    特异性
    This antibody detects endogenous levels of SPT3 protein. (region surrounding Glu203)
    交叉反应 (详细)
    Species reactivity (tested):Human.
    纯化方法
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
    纯度
    > 95 % pure by SDS-PAGE
    Top Product
    Discover our top product SUPT3H Primary Antibody
  • 应用备注
    ELISA: 1: 20000approx. 1: 40000. WB: 1: 500approx. 1: 1000.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 浓度
    1,0 mg/mL
    缓冲液
    Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    DO NOT FREEZE!
    储存条件
    4 °C
    储存方法
    Store the antibody undiluted at 2-8 °C.
  • 抗原
    SUPT3H/SPT3 (SUPT3H) (Suppressor of Ty 3 Homolog (SUPT3H))
    别名
    SUPT3H / SPT3 (SUPT3H 产品)
    别名
    SPT3 antibody, SPT3L antibody, supt3h antibody, MGC75923 antibody, zgc:92246 antibody, SUPT3H antibody, SPT3 homolog, SAGA and STAGA complex component antibody, SPT3 homolog, SAGA and STAGA complex component L homeolog antibody, SUPT3H antibody, supt3h antibody, supt3h.L antibody, Supt3h antibody
    背景
    The transcription of many RNA polymerase II-dependent genes requires Spt3, a member of the S. cerevisiae SAGA complex. Transcription from δ sequences, the long terminal repeats that flank yeast Ty elements, requires the yeast SPT3 gene. Spt3 and Spt20 work together to recruit TATA-box binding protein (TBP) to the core promoter allowing TBP to bind to SAGA-dependent promoters. Null mutations in the Spt3 gene cause defects in sporulation, diploid filamentous growth, and haploid invasive growth, indicating that Spt3 has an important role in both mating and development pathways in yeast. At the promoters of some genes including yeast HO, HIS3 and TRP3 genes, Spt3 inhibits binding of TBP, resulting in reduced transcription. This repressive effect of Spt3 can be overcome by another member of the SAGA complex, GCN5, which promotes the formation of a TBP/TFIIA complex by histone acetylation.Synonyms: SPT3-like protein, SPT3L, Transcription initiation protein SPT3 homolog
    分子量
    approx. 50 kDa
    基因ID
    8464
    NCBI登录号
    NP_003590
    UniProt
    O75486
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