CSF2RA 抗体 (Center)
(1 validation)-
- 抗原 See all CSF2RA 抗体
- CSF2RA (Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA))
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抗原表位
- Center
- 适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CSF2RA antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 特异性
- Recognizes endogenous levels of CD116 protein.
- 产品特性
- Rabbit polyclonal antibody to CD116
- 纯化方法
- The antibody was purified by immunogen affinity chromatography.
- 免疫原
- KLH-conjugated synthetic peptide encompassing a sequence within the center region of human CD116.
- Top Product
- Discover our top product CSF2RA Primary Antibody
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anti-Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA) (AA 1-400) antibody
CSF2RA 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 2G5 unconjugated
anti-Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA) antibody (PE)CSF2RA 适用: 人 FACS 宿主: 小鼠 Monoclonal 4H1 PE
anti-Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA) (AA 101-230) antibodyCSF2RA 适用: 人, 小鼠 IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA) (AA 23-320) antibodyCSF2RA 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA) antibodyCSF2RA 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA) (AA 1-400) antibodyCSF2RA 适用: 人 WB, FACS 宿主: 小鼠 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA) antibodyCSF2RA 适用: 人 WB, IP, FACS, IHC (fro), CyTOF 宿主: 小鼠 Monoclonal 4H1 unconjugated
anti-Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA) (AA 23-150) antibodyCSF2RA 适用: 人 IF 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA) (AA 204-360) antibodyCSF2RA 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA) (AA 23-320) antibodyCSF2RA 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- WB (1:500 - 1:1000)
- 限制
- 仅限研究用
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- by
- Instituto de Parasitología y Biomedicina López-Neyra
- No.
- #100104
- 日期
- 2016.12.23
- 抗原
- CSF2RA
- Lot Number
- Method validated
- Western Blotting
- Positive Control
- primary mouse microglia
- Negative Control
- siRNA knock-down of CSF2RA in primary mouse microglia
- Notes
- Passed. ABIN2705734 specifically recognizes Csf2ra in primary mouse microglia lysates.
- Primary Antibody
- ABIN2705734
- Secondary Antibody
- anti-rabbit HRP-conjugated antibodies (DakoCytomation, P0448, lot 00055814)
- Full Protocol
- Primary mouse microglia are grown in DMEM (Invitrogen), supplemented with 10% foetal bovine serum (Gibco), 10% horse serum and penicillin and streptomycin (Gibco), at 37°C and 5% CO2 in a 12-well dish.
- Transfect cells with 0.05nmol mouse CSF2RA Silencer Select siRNA (ThermoFisher Scientific, ASO1094M, ASO10991 and ASO109DF, lot AMO04BXD) using Lipofectamine 3000 (Invitrogen) following the manufacturer’s instructions.
- Lyse cells in 25µl per well cold lysis buffer (10mM Tris-HCl pH 8.0, 150mM NaCl, 1% Nonidet-P40, 1mM EDTA, 10mM NaF, 1mM Na3VO4, protease inhibitors (Sigma).
- Determine total protein content of the lysates using Bradford assay (Bio-Rad, 500-0006, lot 111832).
- Denature 5µg total protein for 5min at 95°C in 5µl Laemmli SDS sample buffer and subsequently separate them on a denaturing, freshly cast 10% SDS-PAGE for 1h at 140V.
- Transfer proteins onto PVDF membrane (Pall Life Sciences, 75696G, lot TO3225) with a semi-dry Western blotting system for 50min at 60mA.
- Block the membrane with TBST (TBS, 0.1% Tween) containing 5% milk ON at 4°C.
- Incubation with primary rabbit anti-CSF2RA antibody (antibodies-online, ABIN2705734, lot CP1D10A) diluted 1:500 in TBST for 1h at RT.
- Wash membrane 5x 5min with TBST.
- Incubation with anti-rabbit horseradish peroxidase-conjugated secondary antibodies (DakoCytomation, P0448, lot 00055814) diluted 1:2000 in TBST containing 5% milk for 1h at RT.
- Wash membrane 5x 5min with TBST and 3x 5min with TBS.
- Reveal protein bands using ECL Prime Western Blotting Detection Reagent (GE Healthcare, RPN2232, lot 9547481) with Curix RP2 Plus medical X-ray films (AGFA Health Care, ENKMV, lot 79040074) on an AGFA Health Care Curix60-developer.
- Strip membranes using RestoreTM Western Blot Stripping Buffer (Thermo Scientific, 21059, lot NA 164269) and subsequently incubate with a GAPDH loading control antibody (Sigma, G9545, lot 049K4787) diluted 1:10000 and with anti-rabbit horseradish peroxidase-conjugated secondary antibodies (DakoCytomation, P0448, lot 00055814) diluted 1:2000.
- Wash and reveal the protein bands using self-made ECL Western Blotting Detection Reagent with Curix RP2 Plus medical X-ray films (AGFA Health Care, ENKMV, lot 79040074) on an AGFA Health Care Curix60-developer.
- Experimental Notes
- The RND3 antibody ABIN2705734 reveals a protein of the expected molecular weight of mouse Csf2ra in lysates of primary mouse microglia. The protein band’s intensity decreases upon knockdown with two of the three utilized siRNAs.
生效 #100104 (Western Blotting)!['独立验证'标志]( "成功验证")
!['独立验证'标志]( "成功验证")
Validation Images![Primary mouse microglia were transfected with Csf2ra siRNA and Csf2ra was revealed using ABIN2705734 as described in the protocol section (lanes 1, 2, and 3). Lysates from cells transfected with scrambled siRNA (lane 4) and untransfected cells (lane 5) served as negative controls.]()
Primary mouse microglia were transfected with Csf2ra siRNA and Csf2ra was revealed using ABIN2705734 as described in the protocol section (lanes 1, 2, and 3). Lysates from cells transfected with scrambled siRNA (lane 4) and untransfected cells (lane 5) served as negative controls.
Full Methods -
- 状态
- Liquid
- 缓冲液
- Liquid in 0.42 % Potassium phosphate, 0.87 % Sodium chloride, pH 7.3, 30 % glycerol, and 0.01 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- -20 °C
- 储存方法
- Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
- 有效期
- 12 months
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- 抗原
- CSF2RA (Colony Stimulating Factor 2 Receptor, Alpha, Low-Affinity (Granulocyte-Macrophage) (CSF2RA))
- 别名
- CD116 (CSF2RA 产品)
- 别名
- CD116 antibody, CDw116 antibody, CSF2R antibody, CSF2RAX antibody, CSF2RAY antibody, CSF2RX antibody, CSF2RY antibody, GM-CSF-R-alpha antibody, GMCSFR antibody, GMR antibody, SMDP4 antibody, Csfgmra antibody, GM-CSF-Ra antibody, GM-CSFR antibody, colony stimulating factor 2 receptor, alpha antibody, colony stimulating factor 2 receptor alpha subunit antibody, colony stimulating factor 2 receptor, alpha, low-affinity (granulocyte-macrophage) antibody, CSF2RA antibody, Csf2ra antibody
- 背景
- CSF2R, CSF2RY, Granulocyte-macrophage colony-stimulating factor receptor subunit alpha, GM-CSF-R-alpha, GMCSFR-alpha, GMR-alpha, CDw116, CD116
- 基因ID
- 1438, 12982
- UniProt
- P15509, Q00941
- 途径
- JAK/STAT Signaling
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