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MADCAM1 抗体

MADCAM1 适用: 小鼠 FACS, IHC (fro), IHC (f) 宿主: 大鼠 Monoclonal MECA unconjugated
产品编号 ABIN2689829
发货至: 中国
  • 抗原 See all MADCAM1 抗体
    MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))
    适用
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    小鼠
    宿主
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    大鼠
    克隆类型
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    单克隆
    标记
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    This MADCAM1 antibody is un-conjugated
    应用范围
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    Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
    品牌
    BD Pharmingen™
    产品特性
    The MECA-89 antibody reacts with mucosal vascular addressin MAdCAM-1. In the fetus and neonate, MAdCAM-1 is the predominant vascular addressin on the high endothelial venules (HEV) of peripheral lymph nodes. In adult mice, MAdCAM-1 is preferentially expressed in mucosal lymphoid tissues and lamina propria, it is also expressed on sinus-lining cells in the spleen. MAdCAM-1 expression is upregulated on the HEV of peripheral lymph nodes in adult NOD mice and is involved in the development of diabetes and insulitis. Furthermore, there is evidence that IFN-γ can induce MAdCAM-1 expression in non-mucosal sites in adult mice. MAdCAM-1 is a predominant ligand for integrin α4β7, a lymphocyte mucosal homing receptor, and a facultative ligand for CD62L (L-selectin). MECA-89 mAb binds to the second domain of MAdCAM-1 and does not block MAdCAM-1-dependent binding in vitro. Source of the immunogen was endothelial cells from BALB/c mouse mesenteric and peripheral lymph nodes. Immunohistochemical staining of mouse MadCAM-1: Frozen sections of normal mouse small intestine were reacted with the anti-MadCAM-1 antibody. Cells of the lamina propria and the mucosal lymphoid tissue expressing MadCAM-1 can be identified by the brown labeling of their cell membranes. Amplification 20X.

    BD Pharmingen™ Purified Rat Anti-Mouse MAdCAM-1 - Purified - Clone MECA-89 - Isotype Rat IgG2a, κ - Reactivity Ms - 1 mL
    纯化方法
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    免疫原
    Mouse mesenteric and peripheral lymph node cells
    克隆位点
    MECA
    亚型
    IgG2a kappa
    Top Product
    Discover our top product MADCAM1 Primary Antibody
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    31.25 μg/mL
    缓冲液
    Aqueous buffered solution containing BSA, goat serum, and ≤0.09 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Store undiluted at 4°C.
  • Berg, McEvoy, Berlin, Bargatze, Butcher: "L-selectin-mediated lymphocyte rolling on MAdCAM-1." in: Nature, Vol. 366, Issue 6456, pp. 695-8, (1994) (PubMed).

    Berlin, Berg, Briskin, Andrew, Kilshaw, Holzmann, Weissman, Hamann, Butcher: "Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1." in: Cell, Vol. 74, Issue 1, pp. 185-95, (1993) (PubMed).

    Streeter, Berg, Rouse, Bargatze, Butcher: "A tissue-specific endothelial cell molecule involved in lymphocyte homing." in: Nature, Vol. 331, Issue 6151, pp. 41-6, (1988) (PubMed).

  • 抗原
    MADCAM1 (Mucosal Vascular Addressin Cell Adhesion Molecule 1 (MADCAM1))
    别名
    MAdCAM-1 (MADCAM1 产品)
    别名
    MACAM1 antibody, AV211525 antibody, mucosal vascular addressin cell adhesion molecule 1 antibody, MADCAM1 antibody, Madcam1 antibody
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