LGR5 抗体 (Leucine-Rich Repeat Containing G Protein-Coupled Receptor 5) (N-Term) (Biotin)

Details for Product anti-LGR5 Antibody No. ABIN2689805
抗原
  • lgr5-a
  • lgr5a
  • FEX
  • Gpr49
  • GPR49
  • GPR67
  • GRP49
  • HG38
  • leucine-rich repeat containing G protein-coupled receptor 5 L homeolog
  • leucine rich repeat containing G protein coupled receptor 5
  • leucine rich repeat containing G protein-coupled receptor 5
  • lgr5.L
  • Lgr5
  • LGR5
抗原表位
N-Term
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5
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适用

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70
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1
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宿主
大鼠
137
47
克隆类型 (克隆位点)
单克隆 ()
标记
This LGR5 antibody is conjugated to Biotin
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4
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应用范围
BioImaging (BI), Flow Cytometry (FACS), Immunofluorescence (IF)
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选项
品牌 BD Pharmingen™
免疫原 Human LGR5 DNA
克隆位点 8F2
亚型 IgG2b, kappa
产品特性 Lgr5 (leucine-rich-repeat-containing G-protein-coupled receptor 5) is a seven transmembrane-domain receptor that is a target gene for Wnt and marks stem cells in the small intestine, colon, stomach, and hair follicle. Lgr5 was initially identified as a potential stem cell marker due to restricted expression of Lgr5 in the intestinal crypt and labeling of rapidly cycling cells of the colon and intestine. Using both lineage tracing and organoid culture experiments, Lgr5 positive cells are capable of generating all types of the small intestine epithelium hence indicating that Lgr5 marks stem cells of the small intestine and colon. R-spondin growth factors, which are secreted agonists of the Wnt pathway, bind Lgr5. The binding of R-spondins to Lgr5 leads to recruitment of the Frizzled/LRP Wnt receptor complex, which binds to Wnt ligands and leads to downstream Wnt signaling. Lgr5 is up-regulated in colon and ovarian cancers and has been implicated in promotion of tumor growth and metastasis. The 8F2 monoclonal antibody recognizes an epitope in the N-terminal region of Human Lgr5. Flow cytometric analysis of LGR5 expression on human LGR5-transfected cells. LS 174T colorectal adenocarcinoma cells transfected with human LGR5 (cells from Dr. Hans Clevers, Hubrecht Institute, Left Panel) and parental LS 174T cells, which express low levels of endogenous LGR5 (ATCC CL-188, Right Panel) were harvested using Accutase™ Cell Detachment Solution (Cat. No. 561527). The cells were stained with either Biotin Rat IgG2b, κ Isotype Control (Cat. No. 553987, dashed line histogram) or Biotin Rat Anti-Human Lgr5 (N-Terminal) antibody (Cat. No. 562904, solid line histogram) at matched concentrations. The cells were then washed and stained with PE Streptavidin (Cat. No. 554061) as the secondary detection reagent. The fluorescence histograms were derived from gated events based on the light scattering characteristics of viable LS 174T cells. Flow cytometry was performed on a BD FACSCanto™ II

BD Pharmingen™ Biotin Rat anti-Human Lgr5 (N-Terminal) - Biotin - Clone 8F2 - Isotype Rat IgG2b, κ - Reactivity Hu - 50 μg
纯化方法 The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
Plasmids, Primers & others Plasmids, Primers & others LGR5 products on genomics-online (e.g. as negative or positive controls)
抗原
别名 Lgr5 (LGR5 Antibody 摘要)
背景 Synonyms: GPR49, GPR67, HG38
应用备注 Flow Cytometry System.
限制 仅限研究用
浓度 0.5 mg/mL
缓冲液 Aqueous buffered solution containing protein stabilizer and ≤0.09 % sodium azide.
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
储存条件 4 °C
储存方法 Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
有引用在: Kemper, Prasetyanti, De Lau, Rodermond, Clevers, Medema: "Monoclonal antibodies against Lgr5 identify human colorectal cancer stem cells." in: Stem cells (Dayton, Ohio), Vol. 30, Issue 11, pp. 2378-86, 2012 (PubMed).

Yui, Nakamura, Sato, Nemoto, Mizutani, Zheng, Ichinose, Nagaishi, Okamoto, Tsuchiya, Clevers, Watanabe: "Functional engraftment of colon epithelium expanded in vitro from a single adult Lgr5⁺ stem cell." in: Nature medicine, Vol. 18, Issue 4, pp. 618-23, 2012 (PubMed).

Carmon, Gong, Lin, Thomas, Liu: "R-spondins function as ligands of the orphan receptors LGR4 and LGR5 to regulate Wnt/beta-catenin signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 28, pp. 11452-7, 2011 (PubMed).

Merlos-Suárez, Barriga, Jung, Iglesias, Céspedes, Rossell, Sevillano, Hernando-Momblona, da Silva-Diz, Muñoz, Clevers, Sancho, Mangues, Batlle: "The intestinal stem cell signature identifies colorectal cancer stem cells and predicts disease relapse." in: Cell stem cell, Vol. 8, Issue 5, pp. 511-24, 2011 (PubMed).

de Lau, Barker, Low, Koo, Li, Teunissen, Kujala, Haegebarth, Peters, van de Wetering, Stange, van Es, Guardavaccaro, Schasfoort, Mohri, Nishimori, Mohammed, Heck, Clevers: "Lgr5 homologues associate with Wnt receptors and mediate R-spondin signalling." in: Nature, Vol. 476, Issue 7360, pp. 293-7, 2011 (PubMed).

Barker, Huch, Kujala, van de Wetering, Snippert, van Es, Sato, Stange, Begthel, van den Born, Danenberg, van den Brink, Korving, Abo, Peters, Wright, Poulsom, Clevers: "Lgr5(+ve) stem cells drive self-renewal in the stomach and build long-lived gastric units in vitro." in: Cell stem cell, Vol. 6, Issue 1, pp. 25-36, 2010 (PubMed).

Jaks, Barker, Kasper, van Es, Snippert, Clevers, Toftgård: "Lgr5 marks cycling, yet long-lived, hair follicle stem cells." in: Nature genetics, Vol. 40, Issue 11, pp. 1291-9, 2008 (PubMed).

Barker, van Es, Kuipers, Kujala, van den Born, Cozijnsen, Haegebarth, Korving, Begthel, Peters, Clevers: "Identification of stem cells in small intestine and colon by marker gene Lgr5." in: Nature, Vol. 449, Issue 7165, pp. 1003-7, 2007 (PubMed).

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