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CD94 抗体 (Biotin)

KLRD1 适用: 小鼠 FACS, IHC (fro) 宿主: 大鼠 Monoclonal 18d3 Biotin
产品编号 ABIN2689459
发货至: 中国
  • 抗原 See all CD94 (KLRD1) 抗体
    CD94 (KLRD1) (Killer Cell Lectin-Like Receptor Subfamily D, Member 1 (KLRD1))
    适用
    • 57
    • 31
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    小鼠
    宿主
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    • 1
    大鼠
    克隆类型
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    单克隆
    标记
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    This CD94 antibody is conjugated to Biotin
    应用范围
    • 40
    • 30
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    Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
    品牌
    BD Pharmingen™
    产品特性
    The 18d3 antibody reacts with CD94 on all NK cells, NK1.1- or DX5-positive T lymphocytes (NK-T cells), and a subset of CD8-positive T lymphocytes in most strains tested (eg, A/J, AKR/J, BALB/c, C3H/He, C57BL/6, CBA/J, DBA/1, FVB/N, 129/Sv, NOD, SWR, and most DBA/2 substrains, but not DBA/2J). DBA/2J mice do not express CD94.3 CD94 is also expressed on CD8+ T lymphocytes activated in vivo. CD94 is a type-II transmembrane protein with an extracellular lectin-like domain and a short cytoplasmic tail which is not believed to have any signalling function. Heterodimers of CD94 with NKG2A, NKG2C, or NKG2E recognize Qa-1 (a non-classical MHC class I antigen) presenting the Qdm peptide. Studies on CD94/NKG2 heterodimers on human NK cells have demonstrated that the NKG2 components mediate signal transduction for the receptor, NKG2A being inhibitory and NKG2C being stimulatory. Similarly, the mouse NKG2A molecule contains two intracytoplasmic sequences which resemble the ITIM (Immunoreceptor Tyrosine-based Inhibitory Motif) consensus sequence. CD94/NKG2 receptors appear on fetal NK cells before the Ly- 49 MHC class I receptors, suggesting that CD94/NKG2 receptors and their ligand, Qa-1, may play a role in maintenance of self-tolerance in developing NK cells. Expression of CD94 on mouse splenic NK cells. C57BL/6 splenocytes were preincubated with Mouse BD Fc Block™ purified anti-mouse CD16/CD32 mAb 2.4G2 (Cat. no. 553141/553142), then simultaneously stained with biotinconjugated rat IgG2a isotype control mAb R35-95 (Cat. no. 553928, Left panel) or biotinylated mAb 18d3 (Right panel) and APC-conjugated anti- mouse NK-1.1 mAb PK136 (Cat. no. 550627), followed by Streptavidin-PE (Cat. no. 554061). The CD94[dim] and CD94[bright] subpopulations of NK cells are observed in all strains tested, although the relative proportions of the two subsets may vary. Flow cytometry was performed on a BD FACSCalibur™ flow cytometry system.

    BD Pharmingen™ Biotin Rat Anti-Mouse CD94 - Biotin - Clone 18d3 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.1 mg
    纯化方法
    The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
    免疫原
    Transfected cell line
    克隆位点
    18d3
    亚型
    IgG2a kappa
    Top Product
    Discover our top product KLRD1 Primary Antibody
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    限制
    仅限研究用
  • 浓度
    0.5 mg/mL
    缓冲液
    Aqueous buffered solution containing ≤0.09 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
    储存条件
    4 °C
    储存方法
    Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
  • McMahon, Zajac, Jamieson, Corral, Hammer, Ahmed, Raulet: "Viral and bacterial infections induce expression of multiple NK cell receptors in responding CD8(+) T cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 169, Issue 3, pp. 1444-52, (2002) (PubMed).

    Vance, Jamieson, Cado, Raulet: "Implications of CD94 deficiency and monoallelic NKG2A expression for natural killer cell development and repertoire formation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 2, pp. 868-73, (2002) (PubMed).

    Vance, Jamieson, Raulet: "Recognition of the class Ib molecule Qa-1(b) by putative activating receptors CD94/NKG2C and CD94/NKG2E on mouse natural killer cells." in: The Journal of experimental medicine, Vol. 190, Issue 12, pp. 1801-12, (2000) (PubMed).

    Toomey, Salcedo, Cotterill, Millrain, Chrzanowska-Lightowlers, Lawry, Fraser, Gays, Robinson, Shrestha, Dyson, Brooks et al.: "Stochastic acquisition of Qa1 receptors during the development of fetal NK cells in vitro accounts in part but not in whole for the ability of these cells to distinguish between class I-sufficient ..." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 6, pp. 3176-84, (1999) (PubMed).

    Vance, Kraft, Altman, Jensen, Raulet: "Mouse CD94/NKG2A is a natural killer cell receptor for the nonclassical major histocompatibility complex (MHC) class I molecule Qa-1(b)." in: The Journal of experimental medicine, Vol. 188, Issue 10, pp. 1841-8, (1999) (PubMed).

    Ho, Heusel, Brown, Matsumoto, Scalzo, Yokoyama: "Murine Nkg2d and Cd94 are clustered within the natural killer complex and are expressed independently in natural killer cells." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 11, pp. 6320-5, (1998) (PubMed).

    Yokoyama: "Natural killer cell receptors." in: Current opinion in immunology, Vol. 10, Issue 3, pp. 298-305, (1998) (PubMed).

  • 抗原
    CD94 (KLRD1) (Killer Cell Lectin-Like Receptor Subfamily D, Member 1 (KLRD1))
    别名
    CD94 (KLRD1 产品)
    别名
    CD94 antibody, CD94-B antibody, Cd94 antibody, killer cell lectin like receptor D1 antibody, killer cell lectin-like receptor subfamily D, member 1 antibody, killer cell lectin-like receptor, subfamily D, member 1 antibody, KLRD1 antibody, Klrd1 antibody
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