(3 references)-
- 抗原 See all E-cadherin (CDH1) 抗体
- E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This E-cadherin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), BioImaging (BI)
- 品牌
- BD Pharmingen™
- 产品特性
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The 67A4 monoclonal antibody recognizes the extracellular domain of human E-Cadherin (CD324). E-Cadherin is a 120- kDa transmembrane glycoprotein that is localized in the adherens junctions of epithelial cells. There it interacts with the cytoskeleton through the associated cytoplasmic catenin proteins. In addition to being a calcium-dependent adhesion molecule, E-Cadherin is also a critical regulator of epithelial junction formation. Its association with catenins is necessary for cell-to-cell adhesion. These E-Cadherin/catenin complexes associate with cortical actin bundles at both the zonula adherens and the lateral adhesion plaques. Tyrosine phosphorylation can disrupt these complexes, leading to changes in cell adhesion properties. E-Cadherin expression is often down-regulated in highly invasive, poorly differentiated carcinomas. Increased expression of E-Cadherin in these cells reduces their invasiveness. Thus, loss of expression or function of E-Cadherin appears to be an important step in tumorigenic progression. Pluripotent stem cells express E-Cadherin. Upon differentiation, an epithelial to mesenchymal transition results in the loss of E-cadherin expression and a gain in the expression of N-cadherin. Western blot analysis of CD324 (E-cadherin) expression in human breast adenocarcinoma and human embryonic stem (ES) cells. Cell Lysates from a human breast adenocarcinoma cell line MCF-7 (ATCC HTB-22, left blot) and H9 human ES Cells (WiCell, Madison, WI, right blot) were probed with Purified Mouse Anti-Human CD324 (E-Cadherin) monoclonal antibody at titrations of 0.125 (lanes 1), 0.063 (lanes 2), 0.032 (lanes 3) μg/mL. Proteins were detected using HRP Goat Anti-Mouse Ig (Cat. No. 554002). CD324 (E-cadherin) is identified as a band of ~120 kDa in MCF7 and Human ES cells. Immunoflourescent staining of CD324 (E-cadherin) on human embryonic stem (ES) cells. H9 human ES cells (WiCell, Madison, WI) passage 45 grown in mTESR™1 media (StemCell Technologies) on BD Matrigel™ hESC-qualified Matrix (Cat. No. 354277) were fixed with BD Cytofix™ Fixation Buffer (Cat. No. 554655). Cells were stained with Purified Mouse Anti-Human CD324 (E-Cadherin) monoclonal antibody (pseudo-colored green) at 2.5 μg/mL. The second-step reagent was Alexa Fluor® 488 goat anti-mouse Ig (Life Technologies), and cell nuclei were stained with DAPI (pseudo-colored blue). The images were captured on a BD Pathway™ 435 Cell Analyzer and merged using BD Attovision™ software.
BD Pharmingen™ Purified Mouse Anti-Human CD324 (E-Cadherin) - Purified - Clone 67A4 - Isotype Mouse IgG1, κ - Reactivity Hu - 50 μg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Human Breast Tumor Cell Line
- 克隆位点
- 67A4
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product CDH1 Primary Antibody
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anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 401-500) antibody
CDH1 适用: 人, 小鼠, 大鼠 WB, FACS, ELISA, IHC (p), ICC, IHC (fro), IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated
anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) antibodyKO Validated CDH1 适用: 人 WB, IF, IP, IHC (p), ICC, IHC (wm) 宿主: 兔 Polyclonal unconjugated
anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 381-480) antibodyCDH1 适用: 人 WB, ELISA, IHC (p), PLA 宿主: 小鼠 Monoclonal 3F4 unconjugated
anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (C-Term) antibodyCDH1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 810-890) antibodyCDH1 适用: 人, 小鼠, 大鼠 WB, ELISA, IF, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) antibodyCDH1 适用: 人, 小鼠, 大鼠, 猴, 犬 WB, IF, IHC (p), IHC (fro) 宿主: 山羊 Polyclonal unconjugated
anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) antibodyCDH1 适用: 人, 小鼠, 大鼠, Pig, 猴, 犬, 小鸡, 兔, Cow, 马, 豚鼠, Avian, Cat, 驴, 山羊, Hamster, 绵羊 WB, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) antibodyCDH1 适用: 人 WB, IHC, IF, StM, ISt 宿主: 小鼠 Monoclonal CDH1-1525 unconjugated
anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 375-631) antibodyCDH1 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1) (AA 155-707) antibodyCDH1 适用: 人 WB, FACS, ELISA, IF 宿主: 小鼠 Monoclonal 9H2D3 unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "Efficient differentiation of human embryonic stem cells to definitive endoderm." in: Nature biotechnology, Vol. 23, Issue 12, pp. 1534-41, (2005) (PubMed).
: "Adhesion between epithelial cells and T lymphocytes mediated by E-cadherin and the alpha E beta 7 integrin." in: Nature, Vol. 372, Issue 6502, pp. 190-3, (1994) (PubMed).
: "The cadherins: cell-cell adhesion molecules controlling animal morphogenesis." in: Development (Cambridge, England), Vol. 102, Issue 4, pp. 639-55, (1988) (PubMed).
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: "Efficient differentiation of human embryonic stem cells to definitive endoderm." in: Nature biotechnology, Vol. 23, Issue 12, pp. 1534-41, (2005) (PubMed).
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- 抗原
- E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))
- 别名
- CD324 E-Cadherin (CDH1 产品)
- 别名
- Arc-1 antibody, CD324 antibody, CDHE antibody, ECAD antibody, LCAM antibody, UVO antibody, E-cadherin antibody, cdh1 antibody, si:dz180o5.2 antibody, uvo antibody, lcam antibody, E-Cad antibody, XBcad antibody, l-cam antibody, xcdh1 antibody, cdhc-A antibody, xb-cad antibody, XTCAD-1 antibody, c-cadherin antibody, cadherin-1 antibody, uvomorulin antibody, XB-cadherin antibody, arc-1 antibody, cd324 antibody, cdhe antibody, ecad antibody, AA960649 antibody, E-cad antibody, Ecad antibody, L-CAM antibody, Um antibody, cadherin 1 antibody, si:busm1-180o5.2 antibody, cadherin 1, type 1, E-cadherin (epithelial) antibody, cadherin 1, type 1 S homeolog antibody, CDH1 antibody, si:busm1-180o5.2 antibody, cdh1 antibody, Cdh1 antibody, cdh1.S antibody
- 背景
- Synonyms: E-cadherin
- 分子量
- ~120 kDa
- 途径
- WNT signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation
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