(14 references)-
- 抗原 See all FCER2 抗体
- FCER2 (Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This FCER2 antibody is conjugated to Biotin
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应用范围
- Flow Cytometry (FACS)
- 品牌
- BD Pharmingen™
- 产品特性
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The B3B4 antibody reacts with CD23, the low affinity IgE Fc receptor (FcεRII) expressed on mature resting conventional B lymphocytes, but not on B-1 cells (CD5+ B cells) or T lymphocytes. It does not react with high-affinity IgE receptors, as demonstrated on mouse mast cell lines. The regulation of CD23 surface expression on activated B cells appears to be complex, depending upon the mode of activation and the presence of cytokines. IgE synthesis is negatively regulated by CD23, and CD23 expression is upregulated on splenocytes in the presence of IgE. CD23 is also upregulated on follicular dendritic cells in the lymph nodes of immunized mice, and a subset of splenic dendritic cells expresses CD23. The B3B4 antibody abrogates antigen-specific IgE-dependent modulation of immune responses in normal mice. This monoclonal antibody also blocks IgE binding and eosinophil infiltration in the lung of immunized mice. Different in vivo results have been obtained when using the intact B3B4 antibody or the F(ab')2 fragments. B3B4 mAb does not cross-react with rat or human IgE Fc Receptor. This antibody is routinely tested by flow cytometric analysis. Other applications were tested during antibody development only or reported in the literature.
BD Pharmingen™ Biotin Rat Anti-Mouse CD23 - Biotin - Clone B3B4 - Isotype Rat IgG2a, κ - Reactivity Ms - 0.5 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- FcepsilonR isolated from the mouse B hybridoma line O1.2B2
- 克隆位点
- B3B4
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product FCER2 Primary Antibody
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anti-Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2) (AA 151-321) antibody
FCER2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2) (AA 150-330) antibodyFCER2 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2) antibodyFCER2 适用: 人, 小鼠, 大鼠 IF, IHC (p) 宿主: 小鼠 Monoclonal 2F2 unconjugated
anti-Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2) (AA 48-321) antibodyFCER2 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal FCER2-3592 unconjugated
anti-Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2) (AA 1-321) antibodyFCER2 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal S52 unconjugated
anti-Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2) (AA 1-321) antibodyFCER2 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal S51 unconjugated
anti-Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2) (AA 1-321) antibodyFCER2 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2A7 unconjugated
anti-Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2) (AA 48-321) antibodyFCER2 适用: 人 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 2B6 unconjugated
anti-Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2) (AA 48-321) antibodyFCER2 适用: 人 IHC 宿主: 小鼠 Monoclonal MSVA-023M unconjugated
anti-Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2) (AA 50-331) antibodyFCER2 适用: 人, 小鼠, 大鼠 WB, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- The antibody was conjugated with biotin under optimum conditions, and unreacted biotin was removed.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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: "Critical role of CD23 in allergen-induced bronchoconstriction in a murine model of allergic asthma." in: European journal of immunology, Vol. 29, Issue 9, pp. 2957-67, (1999) (PubMed).
: "The expression of murine B cell CD23, in vivo, is regulated by its ligand, IgE." in: International immunology, Vol. 10, Issue 9, pp. 1377-84, (1998) (PubMed).
: "Modulation of antigen-induced B and T cell responses by antigen-specific IgE antibodies." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 8, pp. 4056-63, (1997) (PubMed).
: "Developmental pathways of dendritic cells in vivo: distinct function, phenotype, and localization of dendritic cell subsets in FLT3 ligand-treated mice." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 159, Issue 5, pp. 2222-31, (1997) (PubMed).
: "Central role of immunoglobulin (Ig) E in the induction of lung eosinophil infiltration and T helper 2 cell cytokine production: inhibition by a non-anaphylactogenic anti-IgE antibody." in: The Journal of experimental medicine, Vol. 183, Issue 4, pp. 1303-10, (1996) (PubMed).
: "Mice deficient in CD23 reveal its modulatory role in IgE production but no role in T and B cell development." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 152, Issue 7, pp. 3378-90, (1994) (PubMed).
: "Negative feedback regulation of IgE synthesis by murine CD23." in: Nature, Vol. 369, Issue 6483, pp. 753-6, (1994) (PubMed).
: "Murine follicular dendritic cells and low affinity Fc receptors for IgE (Fc epsilon RII)." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 148, Issue 8, pp. 2340-7, (1992) (PubMed).
: "Loss of CD23 is a consequence of B-cell activation. Implications for the analysis of B-cell lineages." in: Annals of the New York Academy of Sciences, Vol. 651, pp. 130-42, (1992) (PubMed).
: "B-cell subsets defined by the Fc epsilon R." in: Annals of the New York Academy of Sciences, Vol. 651, pp. 84-98, (1992) (PubMed).
: "Expression of B cell surface receptors. II. IL-4 can accelerate the developmental expression of the murine B cell IgE Fc receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 143, Issue 9, pp. 2820-7, (1989) (PubMed).
: "The expression of B cell surface receptors. I. The ontogeny and distribution of the murine B cell IgE Fc receptor." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 140, Issue 7, pp. 2148-54, (1988) (PubMed).
: "Effect of B cell stimulatory factor-1 (interleukin 4) on Fc epsilon and Fc gamma receptor expression on murine B lymphocytes and B cell lines." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 139, Issue 7, pp. 2290-6, (1987) (PubMed).
: "Characterization of a monoclonal antibody directed against the murine B lymphocyte receptor for IgE." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 138, Issue 6, pp. 1845-51, (1987) (PubMed).
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: "Critical role of CD23 in allergen-induced bronchoconstriction in a murine model of allergic asthma." in: European journal of immunology, Vol. 29, Issue 9, pp. 2957-67, (1999) (PubMed).
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- 抗原
- FCER2 (Fc Fragment of IgE, Low Affinity II, Receptor For (CD23) (FCER2))
- 别名
- CD23 (FCER2 产品)
- 别名
- BLAST-2 antibody, CD23 antibody, CD23A antibody, CLEC4J antibody, FCE2 antibody, IGEBF antibody, Fcer2a antibody, Fc fragment of IgE receptor II antibody, FCER2 antibody, Fcer2 antibody
- 背景
- Synonyms: FcεRII
- 途径
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process
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