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驴 anti-山羊 IgG (Heavy & Light Chain) Antibody (PE) - Preadsorbed

WB, FACS, FLISA, FM Polyclonal IgG PE F(ab')2 fragment
产品编号 ABIN2669879
发货至: 中国
  • 抗原 See all IgG products
    IgG
    片段
    F(ab')2 fragment
    抗原表位
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    Heavy & Light Chain
    适用
    • 692
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    山羊
    宿主
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    克隆类型
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    • 1
    多克隆
    标记
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    PE
    应用范围
    • 1837
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    Western Blotting (WB), Flow Cytometry (FACS), FLISA, Fluorescence Microscopy (FM)
    特异性
    Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Phycoerythrin, anti-Donkey Serum, Goat IgG and Goat Serum.
    交叉反应
    山羊
    产品特性
    Anti-Goat F(ab')2 phycoerythrin conjugated antibody generated in donkey detects specifically Goat IgG (H&L). This secondary phycoerythrin conjugated antibody anti-Goat is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
    Anti-Goat F(ab')2 phycoerythrin conjugated antibody generated in donkey detects specifically Goat IgG (H&L). This secondary phycoerythrin conjugated antibody anti-Goat is ideal for investigators who routinely perform titration assays, western-blot, immunoprecipitation and more generally immunoassays.
    纯化方法
    Preadsorption: Solid phase absorption
    免疫原

    Immunogen: Anti-Goat IgG was produced by repeated immunization with goat IgG whole molecule in donkey.

    Immunogen Type: Native Protein

    亚型
    IgG
    Labeling Ratio
    4.74
  • 应用备注

    Application Note: Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate.  Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

    FLISA Dilution: User Optimized

    Flow Cytometry Dilution: 1:100 - 1:250

    Western Blot Dilution: User Optimized

    IF Microscopy Dilution: 1:100 - 1:250

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    0.5 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Product is photosensitive and should be protected from light.
    储存条件
    RT,4 °C
    储存方法
    Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -24 °C or below. This product is stable for several weeks at 4 °C as an undiluted liquid.
    有效期
    12 months
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: Donkey F(ab')2 Anti-Goat IgG Antibody Phycoerythrin Conjugation, Donkey Fab2 Anti-Goat IgG PE Conjugated Antibody

    Background: F(ab')2 Anti-Goat IgG Phycoerythrin Antibody was generated by enzymatic cleavage and subsequent separation from the Fc fragment. Because of their smaller size, F(ab)2 fragments offer several advantages over intact antibodies for use in certain immunochemical techniques and experimental applications. F(ab)2 fragments penetrate tissue samples and show better antigen recognition and signal generation in IHC. F(ab)2 fragments lack the Fc region and therefore do not bind Fc receptors which effectively lowers background staining. F(ab')2 Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.

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