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山羊 anti-兔 IgG (Heavy & Light Chain) Antibody (PE) - Preadsorbed

FACS, FM Polyclonal IgG PE
产品编号 ABIN129765
发货至: 中国
  • 抗原 See all IgG products
    IgG
    抗原表位
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    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Heavy & Light Chain
    适用
    • 692
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    • 557
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    宿主
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    山羊
    克隆类型
    • 3346
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    • 1
    多克隆
    标记
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    PE
    应用范围
    • 1837
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    • 515
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    Flow Cytometry (FACS), Fluorescence Microscopy (FM)
    特异性
    IgG (H&L)
    交叉反应
    产品特性
    Anti-Rabbit IgG (H&L) phycoerythrin conjugated antibody generated in goat detects specifically rabbit IgG. This secondary phycoerythrin conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, microscopy and FACS analysis.
    Concentration Definition: by absorbance = 82.0 at 565 nm
    纯化方法
    Preadsorption: Solid phase absorption
    免疫原

    Immunogen: Anti-Rabbit IgG was produced by repeated immunization with rabbit IgG whole molecule in goat.

    Immunogen Type: Native Protein

    亚型
    IgG
  • 应用备注

    Application Note: Anti-Rabbit IgG (H&L) Phycoerythrin Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate.  Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

    Flow Cytometry Dilution: 1:100 - 1:250

    IF Microscopy Dilution: 1:100 - 1:250

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution Volume: 1.0 mL

    Reconstitution Buffer: Restore with deionized water (or equivalent)

    浓度
    0.5 mg/mL
    缓冲液

    Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

    Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

    Preservative: 0.01 % (w/v) Sodium Azide

    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Product is photosensitive and should be protected from light.
    储存条件
    RT,4 °C
    有效期
    12 months
  • Yasunaga, Ohtsubo, Ohno, Saeki, Kurogi, Tanaka-Okamoto, Ishizaki, Shirai, Mihara, Brock, Miyoshi, Takihara: "Scmh1 has E3 ubiquitin ligase activity for geminin and histone H2A and regulates geminin stability directly or indirectly via transcriptional repression of Hoxa9 and Hoxb4." in: Molecular and cellular biology, Vol. 33, Issue 4, pp. 644-60, (2013) (PubMed).

  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景

    Synonyms: goat anti-Rabbit IgG Antibody, Gt-a-Rabbit Phycoerythrin conjugated, Rabbit IgG (H&L) Antibody in goat, Goat IgG (H&L) Phycoerythrin conjugated Secondary Antibody.

    Background: Anti-Rabbit IgG (H&L) phycoerythrin conjugated antibody generated in goat detects specifically rabbit IgG. This secondary phycoerythrin conjugated antibody anti-Rabbit is ideal for investigators who routinely perform titration assays, microscopy and FACS analysis.

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