EEF2K 抗体
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- 抗原 See all EEF2K 抗体
- EEF2K (Eukaryotic Elongation Factor-2 Kinase (EEF2K))
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适用
- 人, 大鼠, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This EEF2K antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- This antibody detects endogenous levels of EEF-2 kinase / eEF2K protein (region surrounding Pro360).
- 交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - 纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific immunogen
- 纯度
- > 95 % pure by SDS-PAGE
- Top Product
- Discover our top product EEF2K Primary Antibody
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anti-Eukaryotic Elongation Factor-2 Kinase (EEF2K) (Internal Region) antibody
EEF2K 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Elongation Factor-2 Kinase (EEF2K) (N-Term) antibodyEEF2K 适用: 人 WB, IHC (p), IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Elongation Factor-2 Kinase (EEF2K) (AA 1-725) antibodyEEF2K 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
anti-Eukaryotic Elongation Factor-2 Kinase (EEF2K) (AA 406-725) antibodyEEF2K 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Elongation Factor-2 Kinase (EEF2K) (AA 201-500) antibodyEEF2K 适用: 人 ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Elongation Factor-2 Kinase (EEF2K) (Tyr705) antibodyEEF2K 适用: 人, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Elongation Factor-2 Kinase (EEF2K) (AA 331-380), (pSer366) antibodyEEF2K 适用: 人, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Eukaryotic Elongation Factor-2 Kinase (EEF2K) (AA 337-371), (Ser366) antibodyEEF2K 适用: 人, 大鼠 WB 宿主: 兔 Polyclonal RB56632 unconjugated
anti-Eukaryotic Elongation Factor-2 Kinase (EEF2K) (AA 337-371), (pSer366) antibodyEEF2K 适用: 人 WB 宿主: 兔 Polyclonal RB56631 unconjugated
anti-Eukaryotic Elongation Factor-2 Kinase (EEF2K) (pSer366) antibodyEEF2K 适用: 人, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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ELISA: 1: 5000approx. 1: 20000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- EEF2K (Eukaryotic Elongation Factor-2 Kinase (EEF2K))
- 别名
- EEF-2 Kinase (EEF2K 产品)
- 别名
- HSU93850 antibody, eEF-2K antibody, C86191 antibody, SMEF2K antibody, eEF-2 kinase antibody, cb365 antibody, fa04b08 antibody, wu:fa04b08 antibody, zgc:100973 antibody, eukaryotic elongation factor 2 kinase antibody, eukaryotic elongation factor-2 kinase antibody, EEF2K antibody, Eef2k antibody, eef2k antibody, LOC101930123 antibody
- 背景
- The activity of the purified eukaryotic elongation-factor-2 kinase (eEF2K) is completely dependent on calcium and calmodulin, and autophosphorylation on serine and threonine residues is calcium/calmodulin-dependent. eEF2K is a ubiquitous protein kinase that phosphorylates and inactivates eEF2, and thus can modulate the rate of polypeptide chain elongation during translation. eEF2K is 120 kDa protein that is detected in skeletal muscle extracts and is phosphorylated rapidly by SAPK4, but poorly by p38, p38g, JNK or ERK 2. SAPK4 phosphorylates eEF2K at Ser 359 and Ser 396 in vitro, causing its inactivation. The phosphorylation of eEF2K at Ser 359 is also induced by insulin-like growth factor-1. Ser 359 is in close proximity to Ser 366 and the Ser 366 residue also becomes phosphorylated in response to growth factors. eEF2K is phosphorylated by p70 S6 kinase at Ser 366 and this results in the inactivation of eEF2K, especially at low (micromolar) calcium concentrations.Synonyms: Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase, EEF2K, EF2K, Elongation factor 2 kinase
- 分子量
- approx. 105 kDa
- 基因ID
- 29904
- NCBI登录号
- NP_037434
- UniProt
- O00418
- 途径
- AMPK Signaling
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