PDPK1 抗体 (3-phosphoinositide Dependent Protein Kinase-1) (C-Term)

Details for Product anti-PDPK1 Antibody No. ABIN250776
抗原
抗原表位
C-Term, AA 350-436
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适用

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宿主

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克隆类型
多克隆
标记
This PDPK1 antibody is un-conjugated
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应用范围
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
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选项
免疫原 A synthetic peptide made to a C-terminal portion of the human PDK1 protein sequence (between residues 350-436). [Swiss-Prot Q15118]
产品特性 PDK1 (Pyruvate Dehydrogenase Kinase Isoenzyme 1) plays a role in glucose and metabolism of fatty acid regulation via phosphorylation of the pyruvate dehydrogenase subunits. PDK1 also inhibits the activity of pyruvate dehydrogenase and helps regulate metabolite change. PDK1 cellular response to hypoxia is important for cell proliferation under hypoxic conditions. PDK1 also protects against apoptosis in response to oxidative stress and conditions of low oxygen.
纯化方法 Immunogen affinity purified
抗原
别名 PDK-1 (PDPK1 Antibody 摘要)
背景 Gene Symbol: PDK1
分子量 Theoretical MW: 49 kDa
基因ID 5163
UniProt Q15118
途径 PI3K-Akt Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
应用备注 Western Blot 2 μg/mL, Immunocytochemistry/Immunofluorescence 1:1000-1:2000This PDK1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western Blot. By Western blot a band is seen at ~49 kDa, representing PDK1 protein. There are also two higher and one lower non-specific molecular weight bands. In ICC/IF, mitochondiral staining was observed in HeLa cells. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
说明

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

实验流程 Protocol specific for PDK1 Antibody Western Blot Protocol
1. Perform SDS-PAGE (4-12 %) on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to Nitrocellulose according to the instructions provided by the manufacturer of the transfer apparatus.
. Rinse membrane with dH2O and then stain the blot using ponceau S for 1-2 minutes to access the transfer of proteins onto the nitrocellulose membrane. Rinse the blot in water to remove excess stain and mark the lane locations and locations of molecular weight markers using a pencil.
. Rinse the blot in TBS for approximately 5 minutes.
. Block the membrane using 5 % non-fat dry milk + 1 % BSA in TBS for 1 hour at room temperature (RT).
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Dilute the rabbit anti-PDK1 primary antibody in blocking buffer and incubate 1 hour at RT.
. Rinse the membrane in dH2O and then wash the membrane in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each.
. Apply the diluted rabbit-IgG HRP-conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer [TBS + 0.1 % Tween] 3 times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturer's instructions (we used BioFX Super Plus ECL).Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %, provided it does not interfere with antibody-antigen binding.
限制 仅限研究用
状态 Liquid
浓度 1.42 mg/mL
缓冲液 Tris-Glycine and 0.15M NaCl
Buffer contains: 0.05 % Sodium Azide
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Avoid freeze-thaw cycles
储存条件 -80 °C,-20 °C
储存方法 Aliquot and store at -20°C or -80°C. Avoid freeze-thaw cycles.
厂商提供的图像
Image no. 1 for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 350-436), (C-Term) antibody (ABIN250776) Western Blot: PDK-1 Antibody [ABIN250776] - Analysis of HeLa lysates using ABIN250776...
Image no. 2 for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 350-436), (C-Term) antibody (ABIN250776) Detection of PDK1 in human heart lysate using ABIN250776 at 2 ug/ml.
Image no. 3 for anti-3-phosphoinositide Dependent Protein Kinase-1 (PDPK1) (AA 350-436), (C-Term) antibody (ABIN250776) Immunocytochemistry/Immunofluorescence: PDK-1 Antibody [ABIN250776] - Antibody was te...
有引用在: Sung, Chen, Liao, Lee: "Enhanced aerobic glycolysis of nasopharyngeal carcinoma cells by Epstein-Barr virus latent membrane protein 1." in: Experimental cell research, Vol. 359, Issue 1, pp. 94-100, 2017 (PubMed). Method employed by authors: Western Blotting (WB) (Sample species: Human).

Tsoi, Li, Chen, Lau, Tsui, Chan: "The SARS-coronavirus membrane protein induces apoptosis via interfering with PDK1-PKB/Akt signalling." in: The Biochemical journal, Vol. 464, Issue 3, pp. 439-47, 2014 (PubMed). (Sample species: Human). Further details: Immunocytochemistry,Immunofluorescence

Luo, Chang, Zhong, Pandey, Semenza: "Histone demethylase JMJD2C is a coactivator for hypoxia-inducible factor 1 that is required for breast cancer progression." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 49, pp. E3367-76, 2012 (PubMed).

Luo, Hu, Chang, Zhong, Knabel, OMeally, Cole, Pandey, Semenza: "Pyruvate kinase M2 is a PHD3-stimulated coactivator for hypoxia-inducible factor 1." in: Cell, Vol. 145, Issue 5, pp. 732-44, 2011 (PubMed).