MED12 抗体 (Mediator Complex Subunit 12) (C-Term)

Details for Product anti-MED12 Antibody No. ABIN250749, 供应商: Log in to see
抗原
  • ARC240
  • CAGH45
  • FGS1
  • HOPA
  • MED12S
  • OHDOX
  • OKS
  • OPA1
  • TNRC11
  • TRAP230
  • 230kDa
  • Mopa
  • OPA-1
  • Tnrc11
  • Trap230
  • mediator complex subunit 12
  • MED12
  • Med12
  • med12
抗原表位
C-Term
25
8
4
3
2
2
2
2
1
1
1
1
1
适用
人, 小鼠
66
38
16
3
3
3
2
1
1
宿主

55
11
1
克隆类型
多克隆
标记
This MED12 antibody is un-conjugated
4
4
3
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
应用范围
Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
41
39
36
22
18
15
1
选项
Supplier
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Supplier Product No.
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免疫原 The immunogen recognized by this antibody maps to a region between residue 2150 and the C-terminus (residue 2212) of human Mediator of RNA Polymerase II Transcription, subunit 12 (NP_005111.1).
纯化方法 Immunogen affinity purified
Plasmids, Primers & others Plasmids, Primers & others MED12 products on genomics-online (e.g. as negative or positive controls)
抗原 Mediator Complex Subunit 12 (MED12)
别名 MED12 (MED12 Antibody 摘要)
背景 Gene Symbol: MED12
分子量 Theoretical MW: 243 kDa
基因ID 9968
UniProt Q93074
途径 Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Stem Cell Maintenance, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha, Tube Formation
应用备注 Western Blot 1:2000-1:10000, Immunohistochemistry 1:500- 1:2000, Immunocytochemistry/Immunofluorescence 1:250 - 1:1000, Immunoprecipitation 2-5 μg/mg lysate, Immunohistochemistry-Paraffin 1:250The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
说明

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

限制 仅限研究用
状态 Liquid
浓度 1.0 mg/mL
缓冲液 Tris-Citrate/Phosphate ( pH 7.0 - 8.0)
Buffer contains: 0.09 % Sodium Azide
储存液 Sodium azide
注意事项 This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项 Do not freeze.
储存条件 4 °C
储存方法 Store at 4°C. Do not freeze.
厂商提供的图像
Western Blotting (WB) image for anti-Mediator Complex Subunit 12 (MED12) (C-Term) antibody (ABIN250749) anti-Mediator Complex Subunit 12 (MED12) (C-Term) antibody
Western Blotting (WB) image for anti-Mediator Complex Subunit 12 (MED12) (C-Term) antibody (ABIN250749) Western Blot: MED12 Antibody [ABIN250749] - Whole cell lysate (50 ug) from HeLa and m...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Mediator Complex Subunit 12 (MED12) (C-Term) antibody (ABIN250749) Immunohistochemistry-Paraffin: MED12 Antibody [ABIN250749] - Human breast carcinoma.
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Mediator Complex Subunit 12 (MED12) (C-Term) antibody (ABIN250749) Immunohistochemistry-Paraffin: MED12 Antibody [ABIN250749] - FFPE section of human br...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-Mediator Complex Subunit 12 (MED12) (C-Term) antibody (ABIN250749) Immunohistochemistry-Paraffin: MED12 Antibody [ABIN250749] - FFPE section of human br...
Western Blotting (WB) image for anti-Mediator Complex Subunit 12 (MED12) (C-Term) antibody (ABIN250749) Western Blot: MED12 Antibody - Detection of Human and Mouse Med12 by Western Blot. S...
Immunocytochemistry (ICC) image for anti-Mediator Complex Subunit 12 (MED12) (C-Term) antibody (ABIN250749) Immunohistochemistry: MED12 Antibody - Sample: FFPE section of human ovarian carcino...
有引用在: Vogl, Reiprich, Küspert, Kosian, Schrewe, Nave, Wegner: "Sox10 cooperates with the mediator subunit 12 during terminal differentiation of myelinating glia." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 33, Issue 15, pp. 6679-90, 2013 (PubMed). (Sample species: Human). Further details: Western Blotting

Wagner, DeLuca: "Temporal association of herpes simplex virus ICP4 with cellular complexes functioning at multiple steps in PolII transcription." in: PLoS ONE, Vol. 8, Issue 10, pp. e78242, 2013 (PubMed). (Sample species: Primate). Further details: Western Blotting

Miyanari, Torres-Padilla: "Control of ground-state pluripotency by allelic regulation of Nanog." in: Nature, Vol. 483, Issue 7390, pp. 470-3, 2012 (PubMed).

Valdez, Nieto, Murray, Li, Wang, Champlin, Andersson: "Epigenetic modifiers enhance the synergistic cytotoxicity of combined nucleoside analog-DNA alkylating agents in lymphoma cell lines." in: Experimental hematology, Vol. 40, Issue 10, pp. 800-10, 2012 (PubMed).

Langlais, Couture, Sylvain-Drolet, Drouin: "A pituitary-specific enhancer of the POMC gene with preferential activity in corticotrope cells." in: Molecular endocrinology (Baltimore, Md.), Vol. 25, Issue 2, pp. 348-59, 2011 (PubMed).

DAlessio, Ng, Willenbring, Tjian: "Core promoter recognition complex changes accompany liver development." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 10, pp. 3906-11, 2011 (PubMed).

Rocha, Bleiss, Schrewe: "Mosaic expression of Med12 in female mice leads to exencephaly, spina bifida, and craniorachischisis." in: Birth defects research. Part A, Clinical and molecular teratology, Vol. 88, Issue 8, pp. 626-32, 2010 (PubMed). (Sample species: Mouse (Murine)).

Rocha, Scholze, Bleiss, Schrewe: "Med12 is essential for early mouse development and for canonical Wnt and Wnt/PCP signaling." in: Development (Cambridge, England), Vol. 137, Issue 16, pp. 2723-31, 2010 (PubMed). (Sample species: Mouse (Murine)). Further details: Western Blotting

Tutter, Kowalski, Baltus, Iourgenko, Labow, Li, Kadam: "Role for Med12 in regulation of Nanog and Nanog target genes." in: The Journal of biological chemistry, Vol. 284, Issue 6, pp. 3709-18, 2009 (PubMed).

Ding, Tomomori-Sato, Sato, Conaway, Conaway, Boyer: "MED19 and MED26 are synergistic functional targets of the RE1 silencing transcription factor in epigenetic silencing of neuronal gene expression." in: The Journal of biological chemistry, Vol. 284, Issue 5, pp. 2648-56, 2009 (PubMed).

Yokouchi, Nishihara, Harada, Ishida, Yamazaki, Kikuchi, Oizumi, Uramoto, Tanaka, Harada, Akie, Sugaya, Fujita, Takamura, Kojima, Higuchi, Honjo, Minami, Watanabe, Goto, Suzuki, Dosaka-Akita, Isobe et al.: "Immunohistochemical profiling of receptor tyrosine kinases, MED12, and TGF-βRII of surgically resected small cell lung cancer, and the potential of c-kit as a prognostic marker. ..." in: Oncotarget, Vol. 8, Issue 24, pp. 39711-39726, 1970 (PubMed). Method employed by authors: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (Sample species: Human).

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