RAS 抗体 (AA 31-43) (FITC)
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- 抗原 See all RAS products
- RAS
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抗原表位
- AA 31-43
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This RAS antibody is conjugated to FITC
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
- 特异性
- Detects ~21 kDa.
- 交叉反应
- Cow, 人, 小鼠, 大鼠
- 纯化方法
- Protein G Purified
- 免疫原
- Synthetic peptide corresponding to amino acids 31-43 of human RAS. (The immunogen peptide is from the effector binding loop (l2) of the H, K and N-Ras (AA31-43). This sequence is identical yeast, slime mould, fungi, Xenopus, rat, mouse and chicken over these residues.)
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- 应用备注
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- WB (1:1000)
- IHC (1:100)
- ICC/IF (1:200)
- optimal dilutions for assays should be determined by the user.
- 说明
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1 μg/ml of ABIN2485498 was sufficient for detection of Ras in 20 μg of Hela cell lysate by colorimetric immunoblot analysis using goat anti-rabbit IgG:HRP as the secondary antibody.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/mL
- 缓冲液
- PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Conjugated antibodies should be stored at 4°C
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- 抗原
- RAS
- Abstract
- RAS 产品
- 别名
- asr antibody, resistance to audiogenic seizures antibody, ras antibody
- 背景
- The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras and N-Ras) cycle between active (GTPbound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G-protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, in 30 % of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6).
- 基因ID
- 18176
- NCBI登录号
- NP_035067
- UniProt
- B2KGV5
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