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Ubiquitin 抗体 (FITC)

Ubiquitin 适用: Cow WB, IHC, IF, ICC, IP 宿主: 兔 Polyclonal FITC
产品编号 ABIN2481456
发货至: 中国
  • 抗原 See all Ubiquitin 抗体
    Ubiquitin
    适用
    • 148
    • 70
    • 70
    • 68
    • 18
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    • 15
    • 14
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    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Cow
    宿主
    • 108
    • 72
    • 3
    • 2
    • 1
    • 1
    克隆类型
    • 100
    • 86
    多克隆
    标记
    • 80
    • 15
    • 13
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    • 11
    • 7
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    This Ubiquitin antibody is conjugated to FITC
    应用范围
    • 178
    • 104
    • 95
    • 69
    • 59
    • 42
    • 27
    • 21
    • 21
    • 14
    • 13
    • 10
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    Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Immunoprecipitation (IP)
    特异性
    Detects ~10 kDa. It also recognizes ubiquinated proteins.
    交叉反应
    小鸡, Cow, 犬, Drosophila melanogaster, 鱼, 豚鼠, Hamster, 人, 猴, 小鼠, Pig, 兔, Rainbow Trout, 大鼠, Saccharomyces cerevisiae, Salmon, 绵羊, 非洲爪蟾
    纯化方法
    Peptide Affinity Purified
    免疫原
    Native bovine Ubiquitin, conjugated to KLH
    Top Product
    Discover our top product Ubiquitin Primary Antibody
  • 应用备注
    • WB (1:1000)
    • IHC (1:100)
    • ICC/IF (1:100)
    • optimal dilutions for assays should be determined by the user.
    说明

    A 1:1000 dilution of ABIN2481456 was sufficient for detection of free ubiquitin in 15 μg of HeLa lysate by ECL immunoblot analysis using Donkey anti-rabbit IgG:HRP as the secondary antibody.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    PBS pH 7.4, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C
    储存方法
    Conjugated antibodies should be stored at 4°C
  • 抗原
    Ubiquitin
    别名
    Ubiquitin (Ubiquitin 产品)
    别名
    CG11624 antibody, DmUb antibody, DmUbi-p63E antibody, Dmel\\CG11624 antibody, Ub antibody, Ubi-P63e antibody, Ubi-p antibody, Ubi63E antibody, Ubi63p antibody, Ubip antibody, Ubiq antibody, Ubp antibody, ubi antibody, ubi-p63E antibody, ubi63E antibody, CG18282 antibody, CG32744 antibody, CR32744 antibody, DmUbi-p5E antibody, Dmel\\CG32744 antibody, ubiquitin antibody, si:dz180g5.5 antibody, si:busm1-180g5.5 antibody, LOC100194618 antibody, UBIQ antibody, Ubiquitin antibody, LOC100282068 antibody, Ubiquitin-63E antibody, Ubiquitin-5E antibody, transmembrane and ubiquitin-like domain containing 2 antibody, polyubiquitin antibody, Putative ubiquitin family protein antibody, ubiquitin antibody, ubiquitin B antibody, UBiQuitin antibody, Ubiquitin antibody, Ubi-p63E antibody, Ubi-p5E antibody, tmub2 antibody, LOC100194618 antibody, pco110026 antibody, LOC100135798 antibody, UBB antibody, ubq-1 antibody, LOC100190766 antibody
    背景
    Ubiquitin is a small protein that occurs in all eukaryotic cells. The ubiquitin protein itself consists of 76 amino acids and has a molecular mass of about 8.5 kDa. Key features include its C-terminal tail and the 7 Lys residues. It is highly conserved among eukaryotic species: Human and yeast ubiquitin share 96 % sequence identity (1). The main function of Ubiquitin is to clear abnormal, foreign and improperly folded proteins by targeting them for degradation by the 26S proteosome (2). Ubiquitination represents an essential cellular process affected by a multi-enzyme cascade involving classes of enzymes known as ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s or Ubcs) and ubiquitin-protein ligases (E3s). Ubiquitin is activated in a two-step reaction by an E1 ubiquitin-activating enzyme in a process requiring ATP as an energy source. The initial step involves production of an ubiquitin-adenylate intermediate. The second step transfers ubiquitin to the E1 active site cysteine residue, with release of AMP. This step results in a thioester linkage between the C-terminal carboxyl group of ubiquitin and the E1 cysteine sulfhydryl group. The third step is a transfer of ubiquitin from E1 to the active site cysteine of a ubiquitin-conjugating enzyme E2 via a trans(thio)esterification reaction. And the final step of the ubiquitylation cascade creates an isopeptide bond between a lysine of the target protein and the C-terminal glycine of ubiquitin. In general, this step requires the activity of one of the hundreds of E3 ubiquitin-protein ligases (often termed simply ubiquitin ligase). E3 enzymes function as the substrate recognition modules of the system and are capable of interaction with both E2 and substrate(3, 4). Ubiquitination also participates in the internalization and degradation of plasma membrane proteins such as some of the TCR subunits while still ER-membrane associated (5). Ubiquitin also plays a role in regulating signal transduction cascades through the elimination inhibitory proteins, such as IκBα and p27 (6).
    基因ID
    281370
    NCBI登录号
    NP_776558
    UniProt
    P0CG53
    途径
    Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway
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