SART3 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
SART3 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of SART3.
ELISA: Antibody detection limit dilution 1:16,000. Approximately 170 kDa band observed in lysates of cell lines Daudi, Jurkat and MOLT4 (calculated MW of 111 kDa according to NP_055521.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Yang et al, tumor Res. 1999 Aug 15,59 (16) :4056-63, PMID: 10463607). Recommended concentration: 0.01-0.03 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Minimize freezing and thawing.
储存条件
-20 °C
储存方法
Aliquot and store at -20 °C.
抗原
SART3
(Squamous Cell Carcinoma Antigen Recognized By T Cells 3 (SART3))