Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
特异性
This antibody is expected to recognize all reported isoforms (NP_003336.1, NP_919235.1, NP_919236.1 and NP_919237.1).
纯化方法
UBE2I antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
UBE2I antibody was raised against a 13 amino acid synthetic peptide near the N-Terminus of UBE2I.
ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 17 kDa band observed in human kidney, human lung and human testis lysates (calculated MW of 18 kDa according to NP_003336). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human kidney, shows nuclear staining of some epithelial cells of glomeruli and renal tubules. Recommended concentration, 1 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.