DYDC1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
免疫原
DYDC1 antibody was raised against a 13 amino acid synthetic peptide near the internal region of DYDC1 (aa 142-154).
DYDC1
适用: 人
WB, IF (cc), IF (p)
宿主: 兔
Polyclonal
Alexa Fluor 488
应用备注
ELISA: Antibody detection limit dilution 1:128000 Western Blot: Approximately 28 kDa band in rat testis lysates after 1 μg/mL antibody staining.9 kDa according to human NP_620167.1 and of 21.8 kDa according to rat XP_001066918.2. The 28 kDa band was successfully blocked by incubation with the immunizing peptide. In transfected HEK293 transiently expressing human DYDC1 (myc and DYKDDDDK tagged), a band of approximately. 28 kDa is observed. No bands are observed in mock-transfected HEK293 and the same band is observed using anti-myc tag antibody. This 28 kDa band is also observed in rat testis lysates. Recommended concentration, 0.1-1 μg/mL.
限制
仅限研究用
状态
Liquid
浓度
500 μg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.