电话:
400-7060-959
传真:
+86 10 56315212-8813
电子邮件:
orders@antibodies-online.cn

Influenza Nucleoprotein 抗体 (Influenza A Virus H2N2) (H1N1), (H2N2), (H3N2), (H5N1), (H5N2)

NP 适用: Influenza A Virus H2N2 ELISA, WB, IF, IHC, IP, IC 宿主: 小鼠 Monoclonal C43 unconjugated
产品编号 ABIN2452039
发货至: 中国
  • 抗原 See all Influenza Nucleoprotein (NP) 抗体
    Influenza Nucleoprotein (NP)
    抗原表位
    • 15
    • 5
    • 4
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    H1N1, H2N2, H3N2, H5N1, H5N2
    适用
    • 64
    • 40
    • 15
    • 13
    • 11
    • 2
    • 1
    • 1
    • 1
    Influenza A Virus H2N2
    宿主
    • 84
    • 44
    • 4
    小鼠
    克隆类型
    • 86
    • 45
    单克隆
    标记
    • 93
    • 10
    • 10
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    非结合性
    应用范围
    • 86
    • 57
    • 25
    • 15
    • 13
    • 13
    • 10
    • 9
    • 8
    • 7
    • 6
    • 6
    • 5
    • 3
    • 2
    • 2
    • 1
    ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunochromatography (IC)
    特异性
    Reacts with NP of all influenza A viruses, including seasonal H2N2, H3N2, and avian H5N1, H5N2 and H1N1 (seasonal, pandemic and swine).
    交叉反应 (详细)
    No cross reactivity with influenza B viruses.
    纯化方法
    Purified by proprietary chromatography procedure under mild conditions.
    纯度
    90-95 % pure by SDS-PAGE
    过滤
    Sterile filtered
    免疫原
    Human Influenza A Virus (H2N2) Okada strain
    克隆位点
    C43
    亚型
    IgG2a
  • 应用备注
    1) Western blotting: 300~1,000 fold dilution
    2) Immuno-precipitation (100 fold dilution
    3) Immunofluorescent staining (200 fold dilution
    4) Immunohistochemistry (200 fold dilution
    4) ELISA (assay dependent)
    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    1 mg/mL
    缓冲液
    PBS, 50 % glycerol
    储存液
    Azide free
    储存条件
    -20 °C
  • Mizuike, Sasaki, Baba, Iwamoto, Shibai, Kosaka, Kubota-Koketsu, Yang, Du, Sakudo, Tsujikawa, Yunoki, Ikuta: "Development of two types of rapid diagnostic test kits to detect the hemagglutinin or nucleoprotein of the swine-origin pandemic influenza A virus H1N1." in: Clinical and vaccine immunology : CVI, Vol. 18, Issue 3, pp. 494-9, (2011) (PubMed).

    Ueda, Yamate, Du, Daidoji, Okuno, Ikuta, Nakaya: "Maturation efficiency of viral glycoproteins in the ER impacts the production of influenza A virus." in: Virus research, Vol. 136, Issue 1-2, pp. 91-7, (2008) (PubMed).

    Okuno, Isegawa, Sasao, Ueda: "A common neutralizing epitope conserved between the hemagglutinins of influenza A virus H1 and H2 strains." in: Journal of virology, Vol. 67, Issue 5, pp. 2552-8, (1993) (PubMed).

  • 抗原
    Influenza Nucleoprotein (NP)
    别名
    Influenza A (Nucleoprotein) (NP 产品)
    别名
    nucleoprotein antibody, NP antibody
    物质类
    Influenza Protein
    背景
    Influenza virus is an RNA virus, which causes influenza, and belongs to the family Orthomyxoviridae. Influenza virus is classified into three different genera, influenzavirus A, B, and C. They all have similar structures and compositions. The virions are 80-100nm in diameter and usually roughly spherical. The outer surface of the virion is made of a viral envelope containing two major glycoproteins, hemagglutinin (HA) and neuraminidase (NA). Influenzavirus A is further classified into subtypes based on the surface glycoproteins, HA and NA. Currently, there are 16 HA and 9 NA subtypes. The central core of the virion contains the viral RNA genome, which is packaged in the form of ribonucleoprotein complexes. Influenza virus nucleoprotein (NP) is a major component of the ribonucleoprotein complex and is abundantly expressed during the course of infection. It is a structural protein, which encapsidates the negative strand viral RNA and is essential for RNA transcription, replication and packaging. NP binds the PB1 and PB2 subunits of the viral RNA polymerase and the matrix protein M1, in addition to its binding to ssRNA. NP is also known to interact with variety of other macromolecules of both viral and cellular origins, and these interactions have been shown to be essential for the viral lifecycle.
You are here:
客服