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小鼠 anti-Cow IgG Antibody

WB, FACS, IHC (fro) Monoclonal IVA285-1 IgG1 unconjugated
产品编号 ABIN238423
发货至: 中国
  • 抗原 See all IgG products
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    Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
    交叉反应 (详细)
    Species reactivity (tested):Bovine.
    纯化方法
    Protein A affinity chromatography
    免疫原
    Fraction of Bovine immunoglobulin.
    克隆位点
    IVA285-1
    亚型
    IgG1
  • 应用备注
    Western Blotting (Reducing conditions): 0,5-1 μg/mL, overnight in 4C. SDS-PAGE (12 % separating gel). Positive control and sample preparation: BS (bovine serum), dilution 1:50 in Laemmlireducing buffer, boiled in water bath for 3 min. Strongly reacts with bovine IgG light chains, weakly reacts with IgM. Flow Cytometry. Immunohistochemistry (frozen sections).

    Other applications not tested.
    Otimal dilutions are dependent on conditions and should be determined by the user.
    限制
    仅限研究用
  • 浓度
    1.0 mg/mL
    缓冲液
    PBS, pH 7.4, 15 mM Sodium Azide
    储存液
    Sodium azide
    注意事项
    WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    4 °C/-20 °C
    储存方法
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -28 °C for longer.
  • 抗原
    IgG
    Abstract
    IgG 产品
    物质类
    Antibody
    背景
    Synonyms: Bovine Immunoglobulin G
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