LAT2 抗体 (AA 91-243)
-
- 抗原 See all LAT2 抗体
- LAT2 (Linker For Activation of T Cells Family, Member 2 (LAT2))
-
抗原表位
- AA 91-243
-
适用
- 人
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This LAT2 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- The polyclonal antibody recognizes the defined epitope (aa 91-243) of human Non-T cell Activation Linker (NTAL), also known as LAB (linker of activated B cells), a 30 kDa transmembrane adaptor protein present in membrane microdomains (rafts) of B cells, NK cells and myeloid cells.
- 纯化方法
- Protein-A affinity chromatography.
- 免疫原
- Recombinant cytoplasmic domain (aa 91-243) of human NTAL.
- Top Product
- Discover our top product LAT2 Primary Antibody
-
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 91-243), (Cytoplasmic Domain) antibody
LAT2 适用: 人, 小鼠 WB, IP, ICC, FACS 宿主: 小鼠 Monoclonal NAP-07 unconjugated
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) antibodyLAT2 适用: 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 30-209) antibodyLAT2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 91-243), (Cytoplasmic Domain) antibody (PE)LAT2 适用: 人, 小鼠 FACS 宿主: 小鼠 Monoclonal NAP-07 PE
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 32-192) antibodyLAT2 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 196-212) antibodyLAT2 适用: 人 WB, IHC, IP, IHC (p), Inhibition 宿主: 小鼠 Monoclonal NAP-03 unconjugated
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 124-203), (Cytoplasmic Domain) antibodyLAT2 适用: 小鼠 WB 宿主: 兔 Polyclonal unconjugated
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 1-20) antibodyLAT2 适用: 人 IHC (p), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) antibodyLAT2 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Linker For Activation of T Cells Family, Member 2 (LAT2) (AA 91-244) antibodyLAT2 适用: 人, 小鼠 WB 宿主: 小鼠 Monoclonal NAP-07 unconjugated
-
- 应用备注
-
Suitable for Western blot under Non-Reducing conditions: 15 % separating SDS-PAGE gel. Recommended dilution: 1 μg/mL, incubation 60 min in RT, or overnight at 4 °C. Positive control: RAMOS human lymphoma cell line. THP-1 human monocytic leukemia cell line. Negative control: JURKAT human T cell leukemia cell line. Sample preparation: Resuspend approx.50 mil. cells in 1 mLcold Lysis buffer (1 %laurylmaltoside in 20 mM Tris/Cl, 100 mM NaCl pH 8.2, 50 mM NaF including Proteaseinhibitor Cocktail). Incubate 60 min on ice. Centrifuge to remove cell debris. Mix lysatewith non-reducing SDS-PAGE sample buffer. Immunohistochemistry on paraffin sections: 10 μg/mL. Positive tissue: spleen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
-
- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH 7.4 with 15 mM sodium azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
-
- 抗原
- LAT2 (Linker For Activation of T Cells Family, Member 2 (LAT2))
- 别名
- LAT2 / WBSCR15 (LAT2 产品)
- 别名
- LAB antibody, NTAL antibody, WBSCR15 antibody, WBSCR5 antibody, WSCR5 antibody, LAT2 antibody, MGC139435 antibody, Ntal antibody, Wbscr5 antibody, AW125574 antibody, Wbscr15 antibody, linker for activation of T-cells family member 2 antibody, linker for activation of T cells family, member 2 antibody, LAT2 antibody, Lat2 antibody
- 背景
- NTAL (Non-T cell activation linker), also known as LAB (Linker for activation of B cells), is a 30 kDa double-palmitoylated transmembrane adaptor protein expressed by B cells, NK cells, mast cells and macrophages. It is a negative regulator of early stages of BCR-dependent B cell signaling and serves as a negative regulator also in mast cells. However, in mast cells, NTAL also contributes to some activation processes, partially overlapping with LAT function.Synonyms: HSPC046, LAB, LLinker for activation of B-cells, Linker for activation of T-cells family member 2, Membrane-associated adapter molecule, NTAL, Non-T-cell activation linker, WBS15, WBSCR5, Williams-Beuren syndrome chromosomal region 15 protein
- 基因ID
- 7462
- NCBI登录号
- NP_054865
- UniProt
- Q9GZY6
- 途径
- Fc-epsilon Receptor Signaling Pathway, BCR Signaling
-
