CKB 抗体 (Biotin)
(2 references)-
- 抗原 See all CKB 抗体
- CKB (Creatine Kinase, Brain (CKB))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This CKB antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 交叉反应 （详细）
- Cross reactivity: Rabbit : Yes, Mouse : Yes, Rat : Yes
- 过滤
- 0.2 μm filtered
- 克隆位点
- CK-BYK-21E10
- 亚型
- IgG2b
- Top Product
- Discover our top product CKB Primary Antibody
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anti-Creatine Kinase, Brain (CKB) antibody
CKB 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Creatine Kinase, Brain (CKB) (AA 11-367) antibodyCKB 适用: 人 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C4 unconjugated
anti-Creatine Kinase, Brain (CKB) (AA 2-270) antibodyCKB 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Creatine Kinase, Brain (CKB) (AA 66-179) antibodyCKB 适用: 人, 小鼠 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Creatine Kinase, Brain (CKB) (AA 7-381) antibodyCKB 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Creatine Kinase, Brain (CKB) antibodyCKB 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Creatine Kinase, Brain (CKB) (AA 1-381) antibodyCKB 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Creatine Kinase, Brain (CKB) antibodyCKB 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal CPTC-CKB-2 unconjugated
anti-Creatine Kinase, Brain (CKB) antibodyCKB 适用: 人 ELISA, IF, IHC (fro), FACS, Coat 宿主: 小鼠 Monoclonal 2ba6 unconjugated
anti-Creatine Kinase, Brain (CKB) (AA 1-381) antibodyCKB 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 3D5-3D11 unconjugated
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- 应用备注
- For immunohistochemistry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Positive Human Purkinje cells control Negative Human fibroblasts control
- 限制
- 仅限研究用
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- 缓冲液
- PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
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: "Tissue- and cell-specific distribution of creatine kinase B: a new and highly specific monoclonal antibody for use in immunohistochemistry." in: Cell and tissue research, Vol. 280, Issue 2, pp. 435-46, (1995) (PubMed).
: "Production of native creatine kinase B in insect cells using a baculovirus expression vector." in: Molecular and cellular biochemistry, Vol. 143, Issue 1, pp. 59-65, (1995) (PubMed).
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: "Tissue- and cell-specific distribution of creatine kinase B: a new and highly specific monoclonal antibody for use in immunohistochemistry." in: Cell and tissue research, Vol. 280, Issue 2, pp. 435-46, (1995) (PubMed).
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- 抗原
- CKB (Creatine Kinase, Brain (CKB))
- 别名
- Creatine Kinase B-Type (CKB 产品)
- 别名
- B-CK antibody, CKBB antibody, b-ck antibody, ckbb antibody, CKB antibody, Bck antibody, Ck-3 antibody, Ck3 antibody, Ckbb antibody, bck antibody, ckb antibody, cb594 antibody, wu:fj36f10 antibody, wu:fj37d11 antibody, Ckbr antibody, mg:cb02h12 antibody, wu:fb62d04 antibody, wu:fc14g11 antibody, zgc:154095 antibody, zgc:92079 antibody, creatine kinase B antibody, creatine kinase, brain L homeolog antibody, creatine kinase B-type antibody, creatine kinase, brain antibody, creatine kinase, brain b antibody, creatine kinase, brain a antibody, CKB antibody, ckb.L antibody, Ckb antibody, ckb antibody, ckbb antibody, ckba antibody
- 背景
- The monoclonal antibody CK-BYK/21E10 recognizes human creatine kinase B-type, also known as CKB. Human CKB is a protein of 381 amino acids (~45 kDa), expressed in a number of tissues. CKB is most abundant in adult brain, approx. 5-fold lower in the stomach, 10-fold lower in the heart and barely detectable in liver. In brain, whereas most CKB has been shown to be cytosolic, several of the reactions requiring CKB are membrane-associated. CKB belongs to the creatine kinase (CK) isoenzymes that catalyse the synthesis of phosphocreatine (PCr) and its subsequent use in the regeneration of ATP in cell types where the consumption of ATP is rapid and/or sudden. In the brain the different CK isoforms constitute an energy shuttle wherein ATP produced in the mitochondria is used by a mitochondrial CK [e.g. ubiquitous mitochondrial creatine kinase (uMi-CK)] to generate PCr, which is then transported and used by a cytoplasmic CK [e.g. brain creatine kinase (CKB)] to regenerate ATP at discrete cellular sites of high ATP turnover. CKB appears to have a role in regenerating ATP needed for the transport of ions and neurotransmitters since CKB has been localized to brain synaptic plasma membranes, possibly coupled to Na+/K+-ATPase and acetylcholine receptor-rich membranes. Expression of CKB is developmentally controlled: in rat, brain CKB protein at birth is extremely low and increases 10-fold until week 4. This reflects the many energy-demanding processes in brain during brain development. Aliases Creatine kinase B chain, B-CK, BB-CK Immunogen Synthetic 17-mer peptide corresponding to the N-terminal sequence of human creatine kinase brain- type (SNSHNALKLRFPAEDEF)
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