(3 references)-
- 抗原 See all nectin-3 (NECTIN3) 抗体
- nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This nectin-3 antibody is conjugated to FITC
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应用范围
- Flow Cytometry (FACS), Immunofluorescence (IF), Immunoassay (IA)
- 交叉反应 （详细）
- Cross reactivity: Human nectin-3 : No, Rat nectin-3 : No
- 过滤
- 0.2 μm filtered
- 克隆位点
- 103-A1
- 亚型
- IgG2a
- Top Product
- Discover our top product NECTIN3 Primary Antibody
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anti-Nectin cell adhesion molecule 3 (NECTIN3) (AA 282-404) antibody
NECTIN3 适用: 人 WB, ELISA, IHC, FACS, ICC, Neut 宿主: 小鼠 Monoclonal 6D5B2 unconjugated
anti-Nectin cell adhesion molecule 3 (NECTIN3) (AA 84-97), (Extracellular), (N-Term) antibodyNECTIN3 适用: 人, 小鼠, 大鼠 WB, IHC, FACS, IF 宿主: 兔 Polyclonal unconjugated
anti-Nectin cell adhesion molecule 3 (NECTIN3) (AA 59-166) antibodyNECTIN3 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 1D1 unconjugated
anti-Nectin cell adhesion molecule 3 (NECTIN3) (AA 90-293) antibodyNECTIN3 适用: 小鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nectin cell adhesion molecule 3 (NECTIN3) (AA 310-549) antibodyNECTIN3 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nectin cell adhesion molecule 3 (NECTIN3) (Internal Region) antibodyNECTIN3 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nectin cell adhesion molecule 3 (NECTIN3) antibodyNECTIN3 适用: 人, 小鼠 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Nectin cell adhesion molecule 3 (NECTIN3) (AA 1-366) antibodyNECTIN3 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Nectin cell adhesion molecule 3 (NECTIN3) (AA 171-377) antibodyNECTIN3 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Nectin cell adhesion molecule 3 (NECTIN3) (Internal Region) antibodyNECTIN3 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- For immunohistochemistry and flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Positive L-cells (A cultured line of C3H mouse fibroblasts), control Negative Cells/tissues derived from nectin-3 knockout mouse (Ref 5) control
- 限制
- 仅限研究用
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- 缓冲液
- PBS, containing 1.0 % bovine serum albumin and 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
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: "Disruption of ectoplasmic specializations between Sertoli cells and maturing spermatids by anti-nectin-2 and anti-nectin-3 antibodies." in: Asian journal of andrology, Vol. 10, Issue 4, pp. 577-84, (2008) (PubMed).
: "Roles of cell-adhesion molecules nectin 1 and nectin 3 in ciliary body development." in: Development (Cambridge, England), Vol. 132, Issue 7, pp. 1525-37, (2005) (PubMed).
: "Evidence that tubulobulbar complexes in the seminiferous epithelium are involved with internalization of adhesion junctions." in: Biology of reproduction, Vol. 71, Issue 2, pp. 548-59, (2004) (PubMed).
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: "Disruption of ectoplasmic specializations between Sertoli cells and maturing spermatids by anti-nectin-2 and anti-nectin-3 antibodies." in: Asian journal of andrology, Vol. 10, Issue 4, pp. 577-84, (2008) (PubMed).
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- 抗原
- nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))
- 别名
- Nectin-3 (NECTIN3 产品)
- 背景
- The monoclonal antibody 103-A1 recognizes mouse nectin-3. Nectin-3 is a 83 kDa type I transmembrane glycoprotein. Nectin, originally isolated as poliovirus receptor-related protein (PRR), is a cell-cell adhesion molecule of the immunoglobulin supergene family. Nectins are calcium- independent immunoglobulin-like cell-cell adhesion molecules consisting of four members, nectin 1-4. Nectins homophilically and heterophilically trans-interact to form a variety of cell-cell junctions, including cadherin-based adherens junctions in epithelial cells and fibroblasts in culture, synaptic junctions in neurons, and Sertoli cell-spermatid junctions in testis, in cooperation with, or independently of, cadherins. Both nectin-2 and nectin-3 are ubiquitously expressed, whereas nectin-1 is abundantly expressed in brain. Nectin-2 and -3 are expressed in cells where cadherin is not expressed, such as blood cells and spermatids. All members of the nectin family have two or three splice variants. For nectin-3, three isoforms exist: nectin-3α, -3β and -3γ of which nectin-3α is the largest. Nectin-3, also known as PRR3, is a transmembrane protein that is predominantly expressed in testis and placental tissues as well in many cell lines. Nectin interacts in vivo with both long and short isoforms of afadin, an actin binding protein, at cadherin-based cell-cell adherence junctions in various tissues and cell lines. Furthermore, the ectodomains of nectin-3 and CD155 (Poliovirus Receptor) have shown strong affinity to each other. Injection of antibody 103-A1 into lumen of seminiferous tubules leads to disruption of the actin filaments in Sertoli cells at the Sertoli-maturing spermatid ectoplasmic specialization and exfoliation of maturing spermatids form the seminiferous epithelium. Aliases PPR3, poliovirus receptor-related protein 3, PRR3, PVRR3, CD113 Immunogen Recombinant mouse nectin-3 extracellular domain (50-400 aa) fused to IgG Fc
- 途径
- Cell-Cell Junction Organization
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